Genetic and morphologic differentiation of the Lusitanian toadfish (<i>Halobatrachus didactylus</i>) between estuarine and coastal areas in Portugal

Joana F. Marques; Ana Luísa Rego; José Lino Costa; Maria José Costa; Henrique N. Cabral

doi:10.3989/scimar.2006.70n4749

Autores/as

Joana F. Marques Universidade de Lisboa, Faculdade de Ciências, Instituto de Oceanografia, Lisboa
Ana Luísa Rego Universidade de Lisboa, Faculdade de Ciências, Instituto de Oceanografia, Lisboa
José Lino Costa Universidade de Lisboa, Faculdade de Ciências, Instituto de Oceanografia, Lisboa
Maria José Costa Universidade de Lisboa, Faculdade de Ciências, Instituto de Oceanografia, Lisboa
Henrique N. Cabral Universidade de Lisboa, Faculdade de Ciências, Instituto de Oceanografia, Lisboa

DOI:

https://doi.org/10.3989/scimar.2006.70n4749

Palabras clave:

Halobatrachus didactylus diferenciación, alozimas, morfología, estructura poblacional, áreas costeras

Resumen

El pejesapo, Halobatrachus didactylus (Bloch y Schneider, 1801), está distribuido desde la costa de Ghana hasta la Península Ibérica, siendo particularmente abundante en la costa sur portuguesa. La diferenciación de esta especie a lo largo de la costa Portuguesa se ha evaluado a través del análisis de diez muestras, considerando caracteres morfológicos (20 características morfométricas y 16 merísticas) y genéticos (10 aloenzimas, 11 loci). Hacia el sur, las muestras incluyen estuarios y sus áreas costeras adyacentes, ya que esta especie habita ambos ambientes, mientras que las muestras del oeste están relacionadas sólo a estuarios. El análisis discriminante de los datos morfométricos y merísticos evidenciaron diferenciación entre poblaciones de estuarios y costeras, lo cual no fue enteramente corroborado por el análisis genético, el cual mostró un patrón general de bajo F_ST (0.042) y distancia genética de Nei, incluyendo áreas geográficamente distantes. Sin embargo, valores más altos de estos parámetros fueron encontrados entre estuarios de la costa sur y sus áreas costeras adyacentes, sugiriendo que los sistemas de estuarios juegan un papel importante en tal diferenciación. Los resultados son discutidos considerando los patrones de historia de vida del pejesapo y la geomorfología de la costa portuguesa, dando una perspectiva de cómo los procesos biológicos y factores ambientales influyen la sub- estructuración poblacional.

Descargas

Los datos de descargas todavía no están disponibles.

Citas

Alayse, J.P. – 1987. Le complexe Solea lascaris: mise en evidence par l’étude du polymorphisme enzymatique de deux espèces sympatriques en Mer d’Iroise. Biochem. Syst. Ecol., 15: 204–273. doi:10.1016/0305-1978(87)90031-7

Alexandrino, P. – 1996. Genetic and morphological differentiation among some Portuguese populations of allis shad Alosa alosa (L., 1758) and twaite shad Alosa fallax (Lacépède, 1803). Pub. Espec. Inst. Esp. Oceanog., 21: 15-24.

Avise, J.C., C.A. Reeb and N.C. Saunders. – 1987. Geographic population structure and species differences in mitochondrial DNA of mouthbrooding marine catfishes (Ariidae) and demersal spawning toadfishes (Batrachoididae). Evolution, 41: 991-1002. doi:10.2307/2409187

Ayvazian, S.G., M.S. Johnson and D.J. McGlashan. – 1994. High levels of genetic subdivision of marine and estuarine populations of the estuarine catfish Cnidoglanis macrocephalus (Plotosidae) in southwest Australia. Mar. Biol., 118: 25-31. doi:10.1007/BF00699216

Beheregaray, L.B. and P. Sunnucks. – 2001. Fine-scale genetic structure, estuarine colonization and incipient speciation in the marine silverside fish Odontesthes argentinensis. Mol. Ecol., 10: 2849-2866. doi:10.1046/j.1365-294X.2001.t01-1-01406.x

Belkhir, K., P. Borsa, L. Chikhi, N. Rafauste and F. Bonhomme. – 1996-2001. GENETIX 4.02, logiciel sous Windows TM pour la génétique des populations. Université de Montpellier II, Montpellier.

Bilton, D.T., J. Paula and J.D.D. Bishop. – 2002. Dispersal, genetic differentiation and speciation in estuarine organisms. Est. Coast. Shelf Sci., 55: 937-952. doi:10.1006/ecss.2002.1037

Cabral, H.N., F. Castro, D. Linhares and P. Alexandrino. – 2003a. Genetic differentiation of Solea solea (Linnaeus, 1758) and Solea senegalensis Kaup, 1858, (Pisces: Pleuronectiformes) from several estuarine systems of the Portuguese coast. Sci. Mar., 67: 43-52.

Cabral, H.N., J.F. Marques, A.L. Rego, A.I. Catarino, J. Figueiredo and J. Garcia. – 2003b. Population differentiation of Synaptura lusitanica Capello, 1868, along the Portuguese coast. J. Sea Res., 50: 167-175. doi:10.1016/S1385-1101(03)00060-1

Castilho, R. and B.J. McAndrew. – 1998. Population structure of seabass in Portugal: evidence from allozymes. J. Fish Biol., 53: 1038-1049. doi:10.1111/j.1095-8649.1998.tb00461.x

Chaplin, J.A., G.A. Baudains, H.S. Gill, R. McCulloch and I.C. Potter. – 1998. Are assemblages of black bream (Acanthopagrus butcheri) in different estuaries genetically distinct? Int. J. Salt Lake Res., 6: 303-321. doi:10.1007/BF02447913

Chikhi, L., F. Bonhomme and J.-F. Agnese. – 1998. Low genetic variability in a widely distributed and abundant clupeid species, Sardinella aurita. New empirical results and interpretations. J. Fish Biol., 52: 861-878.

Costa, J.L. – 1993. Abundance and distribution of the toadfish Halobatrachus didactylus on the Mira estuary (Portugal): seasonal variations and ruling factors. J. Fish Biol., 43 (Suppl. A): 330.

Goudet, J., S. Raymond, T. de Meeüs and F. Rousset. – 1996. Testing differentiation in diploid populations. Genetics, 144: 1933-1940.

Gyllensten, U. – 1985. The genetic structure of fish: differences in the intraspecific distribution of biochemical genetic variation between marine, anadromous and freshwater species. J. Fish Biol., 26: 691-699. doi:10.1111/j.1095-8649.1985.tb04309.x

Hair Jr., J.F., R. Anderson, R. Tatham and W. Black. – 1996. Multivariate data analysis with readings. Prentice Hall Incorporated, New Jersey.

Harris, H. and D.A. Hopkinson. – 1976. Handbook of enzyme electrophoresis in human genetics. North-Holland publishers, Amesterdam.

Humphries, J.M., F.L. Bookstein, B. Chernoff, G.R. Smith, R.L. Elder and S.G. Poss. – 1981. Multivariate discrimination by shape in relation to size. Syst. Zool., 30: 291-308. doi:10.2307/2413251

Hurlbut, T. and D. Clay. – 1998. Morphometric and meristic differences between shallow- and deep-water populations of White hake (Urophycis tenuis) in the southern Gulf of St Lawrence. Can. J. Fish. Aquat. Sci., 55: 2274-2282. doi:10.1139/cjfas-55-10-2274

Ihssen, P.E., D.O. Evans, W.J. Christie, J.A. Reckahn and R.L. DesJardine. – 1981. Life history, morphology, and electrophoretic characteristics of five allopatric stocks of Late whitefish (Coregonus clupeaformis) in the Great Lakes region. Can. J. Fish. Aquat. Sci., 38: 1790-1807. doi:10.1139/f81-226

Kinsey, S.T., T. Orsoy, T.M. Bert and B. Mahmoudi. – 1994. Population structure of the Spanish sardine Sardinella aurita: natural morphological variation in a genetically homogeneous population. Mar. Biol., 118: 309-317. doi:10.1007/BF00349798

Lindsey, C.C. – 1988. Factors controlling meristic variation. Fish Physiol., 11: 197-274. doi:10.1016/S1546-5098(08)60215-0

Mantel, N. – 1967. The detection of disease clustering and a generalized regression approach. Cancer Res., 27: 209-220.

Misra, R.K. and J.E. Carscadden. – 1987. A multivariate analysis of morphometrics to detect differences in populations of Capelin (Mallotus villosus). J. Cons. Int. Explor. Mer, 43: 99-106.

Murphy, R.W., J.W. Sites, D.G. Burth Jr. and C.H. Haufler. – 1996. Proteins: Isozyme electrophoresis. In: D.M. Hills, C. Moritz and B.K. Mable (eds.), Molecular systematics, pp. 51-120. Sinauer Associates, Massachussets.

Murta, A.G. – 2000. Morphological variation of Horse mackerel (Trachurus trachurus) in the Iberian and North African Atlantic: implications for stock identification. ICES J. Mar. Sci., 57: 1240-1248. doi:10.1006/jmsc.2000.0810

Nei, M. – 1978. Estimation of the average heterozygosity and genetic distance from a small number of individuals. Genetics, 89: 583-590.

Nielsen, E.E., P.H. Nielsen, D. Meldrup and M.M. Hansen. – 2004. Genetic population structure of turbot (Scophthalmus maximus L.) supports the presence of multiple hybrid zones for marine fishes in the transition zone between the Baltic Sea and North Sea. Mol. Ecol., 13: 585-595. doi:10.1046/j.1365-294X.2004.02097.x

Pampoulie, C., E.S. Gysels, G.E. Maes, B. Hellemans, V. Leentjes, A.G. Jones and F.A.M. Volchaert. – 2004. Evidence for finescale genetic structure and estuarine colonisation in a potential high gene flow marine goby (Pomatischistus minutos). Heredity, 92: 434-445. doi:10.1038/sj.hdy.6800438

Pasteur, N., M. Autem, P. Pichot and M. Goucha. – 1985. Structure génétique de la sole (Solea vulgaris Quensel, 1806; Téléostéens, Soléidés): premier catalogue de polymorphismes biochemiques acessibles par l’electrophorèse en gel d’amidon. Rev. Trav. Inst. Pêches Mar., 47: 37-54.

Pinheiro, A., C.M. Teixeira, A.L. Rego, J.F. Marques and H.N. Cabral. – 2005. Genetic and morphological variation of Solea lascaris (Risso, 1810) along the Portuguese coast. Fish. Res., 73: 67-78. doi:10.1016/j.fishres.2005.01.004

Raymond, M. and F. Rousset. – 1995. GENEPOP: population genetics software for exact tests and ecumenism. J. Heredity, 86: 248-249.

Ricker, W.E. – 1973. Linear regressions in fishery research. J. Fish. Res. Board Can., 30: 409-434.

Roldán, M.I., R.G. Perrotta, M. Cortey and C. Pla. – 2000. Molecular and morphologic approaches to discrimination of variability patterns in Chub mackerel, Scomber japonicus. J. Exp. Mar. Biol. Ecol., 253: 63-74. doi:10.1016/S0022-0981(00)00244-6

Roux, C. – 1986. Batrachoididae. In: P.J.P. Whitehead, M.L. Bauchot, J.C. Hureau, J. Nielsen and E. Tortonese. (eds.) Fishes of the North-eastern Atlantic and Mediterranean, vol. III, 1360-1361. UNESCO, Paris.

Santos, M.E., T. Modesto, R.J. Matos, M.S. Grober, R.F. Oliveira and A. Canário. – 2000. Sound production by the Lusitanian toadfish, Halobatrachus didactylus. Bioacoustics, 10: 309-321.

Shaklee, J.B., F.W. Allendorf, D.C. Moritz and G.S. Whitt. – 1990. Gene nomenclature for protein-coding loci in fish. Trans. Amer. Fish. Soc., 119: 2-15. doi:10.1577/1548-8659(1990)119<0002:GNFPLI>2.3.CO;2

Smith, P.J. and Y. Fujio. – 1982. Genetic variation in marine teleosts: high variability in habitat specialists and low variability in habitat generalists. Mar. Biol., 69: 7-20. doi:10.1007/BF00396955

Sobral, D. and J. Gomes. – 1997. Peixes litorais. Estuário do Sado. ICN, Lisboa.

Spanakis, E., N. Tsimenides and E. Zouros. – 1989. Genetic differences between populations of sardine, Sardina pilchardus, and anchovy, Engraulis encrasicolus, in the Aegean and Ionian seas. J. Fish Biol., 35: 417-437. doi:10.1111/j.1095-8649.1989.tb02993.x

Stefanni, S., P.J. Miller and P. Torricelli. – 1996. Studio comparativo di due popolazioni di Pomatoschistus minutus (Teleostei: Gobiidae) della laguna veneta e di Plymouth (UK). Atti Conveg. Assoc. Ital. Ittiol. Acque Dolci (AIIAD), 4: 385-390.

Stefanni, S., E.S. Gysells, F.A.M. Volckaert and P.J. Miller. – 2003. Allozyme variation and genentic divergence in the sand goby, Pomatoschistus minutus (Teleostei: Gobiidae). J. Mar. Biol. Assoc. U.K., 83: 1143-1149. doi:10.1017/S0025315403008403h

Tudela, S. – 1999. Morphological variability in a Mediterranean, genetically homogeneous population of the European anchovy, Engraulis encrasicolus. Fish. Res., 42: 229-243. doi:10.1016/S0165-7836(99)00052-1

Ward, R.D., M. Woodwark and O.F. Skibinski. – 1994. A comparison of genetic diversity levels in marine, freshwater and anadromous fishes. J. Fish Biol., 44: 213-232. doi:10.1111/j.1095-8649.1994.tb01200.x

Weir, B.S. and C.C. Cockerham. – 1984. Estimating F-statistics for the analysis of population structure. Evolution, 38: 1358-1370. doi:10.2307/2408641

Wright, S. – 1965. The interpretation of population structure by Fstatistics with special regard to systems of mating. Evolution, 19: 395-420. doi:10.2307/2406450