Reproductive phase determination in male meagre (Argyrosomus regius, Sciaenidae): testis development and histologic corroboration of a gross anatomical scale


  • Nuno Prista Centro de Oceanografia, Faculdade de Ciências da Universidade de Lisboa - Departamento de Biologia Animal, Faculdade de Ciências da Universidade de Lisboa - Instituto Português do Mar e da Atmosfera
  • Leonel Gordo Centro de Oceanografia, Faculdade de Ciências da Universidade de Lisboa - Departamento de Biologia Animal, Faculdade de Ciências da Universidade de Lisboa
  • José Lino Costa Centro de Oceanografia, Faculdade de Ciências da Universidade de Lisboa
  • Maria José Costa Centro de Oceanografia, Faculdade de Ciências da Universidade de Lisboa - Departamento de Biologia Animal, Faculdade de Ciências da Universidade de Lisboa
  • Cynthia Jones Center for Quantitative Fisheries Ecology, Old Dominium University



histology, testes, reproduction, males, validation, Argyrosomus regius


Reproductive stage determination of male gonads has received sparse attention in fish biology literature with few studies detailing the building of gross anatomical- and histologic scales. The meagre (Argyrosomus regius) is one of the world’s largest sciaenids and supports a significant regional fishery in European and North African waters whose reproductive patterns are yet to be fully investigated. In the present study, we derive a macroscopic grading system for meagre testis using semi-quantitative graphs that feature the testis variability along the species size range and time of the year. We then describe the histological stages and reproductive phases of male testes and determine the extent to which they corroborate the anatomical scale. Our results indicate that gross anatomical analyses are accurate in assessments of the meagre spawning season but may not precisely distinguish the testes of well-mature fish and first spawning virgins. Furthermore, we show that milt expression varies widely with size and misclassifies as immature many smaller fish in spawning-capable condition. We discuss these findings in terms of their contribution to the understanding of testes development and the uncertainties involved in determining the size-at-maturity of male fish using gross anatomical scales.


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Abou Shabana N.M., Abd El Rahman S.H., Al Absawy M.A., Assem S.S. 2012. Reproductive biology of Argyrosomus regius (Asso, 1801) inhabiting the south eastern Mediterranean Sea, Egypt. Egyptian J. Aquat. Res. 38: 147-156.

Almeida F.F.L., Kristoffersen C., Taranger G.L., Schulz R.W. 2008. Spermatogenesis in Atlantic cod (Gadus morhua): A novel model of cystic germ cell development. Biol. Reprod. 78: 27-34. PMid:17881768

Brown-Peterson N.J., Wyanski D.M., Saborido-Rey F., Macewicz B.J., Lowerre-Barbieri S.K. 2011. A standardized terminology for describing reproductive development in fishes. Mar. Coast. Fish. 3: 52-70.

Costa A.M. 2009. Macroscopic vs. microscopic identification of the maturity stages of female horse mackerel. ICES J. Mar. Sci. 66: 509-516.

Costa M.J., Cabral H., Costa J.L., Prista N., Lopes J.C. 2008. Corvina-legítima Argyrosomus regius: Dados biológicos para a gestão e produção aquícola de um recurso. Final Report (Project Ref: DGPA-MARE: 22-05-01-FDR-00036). Centro de Oceanografia, Faculdade de Ciências da Universidade de Lisboa, Lisboa, Portugal. [in Portuguese].

Dadzie S., Abou-Seedo F. 2004. Testicular structure and spawning cycle in the silvery croaker, Otolithes ruber (Perciformes: Sciaenidae) in the Kuwaiti waters of the Arabian Gulf. Ichthyol. Res. 51: 263-268.

FAO, 2009. Capture Production 1950–2007. FISHSTAT Plus: Universal software for fishery statistical time series. Version 2.3. FAO Fisheries Department, Fishery Information, Data and Statistics Unit. FAO, Rome, Italy.

Farmer B. 2003. Biology of the Mulloway Argyrosomus japonicus in Western Australia. M.Sc. thesis, Murdoch Univ., 107 pp.

Farmer B. 2008. Comparisons of the biological and genetic characteristics of the Mulloway Argyrosomus japonicus (Sciaenidae) in different regions of Western Australia. Ph.D. thesis, Murdoch Univ., 217 pp.

Ferreri R., Basilone G., D'Elia M., Traina A., Saborido-Rey F., Mazzola S. 2009. Validation of macroscopic maturity stages according to microscopic histological examination for European anchovy. Mar. Ecol. 30: 181-187.

Gil M.M., Grau A., Basilone G., Ferreri R., Palmer M. 2013. Reproductive strategy and fecundity of meagre Argyrosomus regius Asso, 1801 (Pisces: Sciaenidae): implication for restocking programs. Sci. Mar. 77: 105-118.

González-Quirós R.R., Del Arbol J., Garcia-Pacheco M.D.M., Silva-Garcia A.J., Naranjo J.M., Morales-Nin B. 2011. Life-history of the meagre Argyrosomus regius in the Gulf of Cádiz (SW Iberian Peninsula). Fish. Res. 109: 140-149.

Grau A., Linde M., Grau A.M. 2009. Reproductive biology of the vulnerable species Sciaena umbra Linnaeus, 1758 (Pisces: Sciaenidae). Sci. Mar. 73: 37-81.

Grier H.J. 2002. The germinal epithelium: Its dual role in establishing male reproductive classes and understanding the basis for indeterminate egg production in female fishes. In: Creswell R.L. (ed.), Proceedings of the Fifty-Third Annual Gulf and Caribbean Fisheries Institute. Mississipi/Alabama Sea Grant Consortium Fort Pierce, Florida, pp. 537-552.

Grier H.J., Taylor R.G., Reese R.O. 1987. The mechanism of tubule elongation during testicular recrudescence in the redfish, Sciaenops ocellatus (Perciformes). In: Kullander S.O., Femholn B. (eds), Proceedings of the 5th Congress of European Ichthyologists, Swedish Museum of Natural History, Stockholm, pp. 285-291.

Griffiths M.H. 1996. Life history of the dusky kob Argyrosomus japonicus (Sciaenidae) off the East coast of South Africa. S. Afr. J. Mar. Sci. 17: 135-154.

Griffiths M.H. 1997. Life history and stock separation of silver kob Argyrosomus inodorus in South African waters. Fish. Bull. 95: 47-67.

Hermas J. 1995. Contribuition à l'étude des débarquements et de la biologie du maigre Argyrosomus regius (Asso, 1801) au port d'Agadir. Ph.D. thesis, Univ. Ibnou Zohr, 113 pp.

Hunter J.R., Macewicz B.J. 2003. Improving the accuracy and precision of reproductive information used in fisheries. In: Kjesbu O.S., Hunter J.R., Witthames P.R. (eds), Report of the working group on modern approaches to assess maturity and fecundity of warm- and cold-fish and squids. John Grieg Grafisk AS, Bergen, pp. 57-68.

Lowerre-Barbieri S.K., Ganias K., Saborido-Rey F, Murua H., Hunter J.R. 2011. Reproductive timing in marine fishes: Variability, temporal scales, and methods. Mar. Coast. Fish. 3: 71-91.

Morales-Nin B., Geffen A.J., Pérez-Mayol S., Palmer M., González-Quirós R., Grau A. 2012. Seasonal and ontogenic migrations of meagre (Argyrosomus regius) determined by otolith geochemical signatures. Fish. Res. 127-128: 154-165.

Moravec F., Prista N., Costa M.J. 2007. Meagre, Argyrosomus regius (Osteichthyes), as host of a gonad-infecting species of Philometra (Nematoda: Philometridae) off the Atlantic coast of Portugal. Dis. Aquat. Org. 78: 83-86. PMid:18159676

Murphy M.D., Taylor R. 1989. Reproduction and growth of black drum, Pogonias cromis, in Northeast Florida. NE Gulf Sci. 10: 127-137.

Murphy M.D., Taylor R. 1990. Reproduction, growth, and mortality of red drum Sciaenops ocellatus in Florida waters. Fish. Bull. 88: 531-542.

Nieland D.L., Wilson C.A. 1993. Reproductive biology and annual variation of reproductive variables of black drum in the North ern Gulf of Mexico. Trans. Am. Fish. Soc. 122: 318-327.<0318:RBAAVO>2.3.CO;2

Parenti L.R., Grier H.J. 2004. Evolution and phylogeny of gonad morphology in bony fishes. Integr. Comp. Biol. 44: 333-348. PMid:21676719

Prista N., Costa J.L., Costa M.J., Jones C.M. 2007. New methodology for studying large valuable fish in data-poor situations: Commercial mark-recapture of meagre Argyrosomus regius in the southern coast of Portugal. ICES CM/O: 43, 18 pp.

Prista N., Jones C.M., Costa J.L., Costa M.J. 2008. Inferring fish movements from small-scale fisheries data: The case of Argyrosomus regius (Sciaenidae) in Portugal. ICES CM/K: 19, 19 pp.

Prista N., Costa J.L., Costa M.J., Jones C.M. 2009. Age determination in meagre, Argyrosomus regius. Rel. Cient. Téc. Inst. Invest. Pescas Mar 49: 1-54.

Prista N., Diawara N., Costa M.J., Jones C.M. 2011. Using SARIMA to assess data-poor fisheries: Case-study with a sciaenid fishery off Portugal. Fish. Bull. 109: 170-185.

Quéméner L. 2002. Le maigre commun (Argyrosomus regius) - Biologie, pêche, marché et potentiel aquacole. Éditions Ifremer, Plouzané, 31 pp.

Quéméner L., Suquet M., Mero D., Gaignon J.-L. 2002. Selection method of new candidates for finfish aquaculture: The case of the French Atlantic, the Channel and the North Sea Coasts. Aquat. Living Resour. 15: 293-302.

Quéro J.-C., Vayne J.-J. 1987. Le maigre, Argyrosomus regius (Asso, 1801) (Pisces, Perciformes, Sciaenidae) du Golfe de Gascogne et des eaux plus septentrionales. Rev. Trav. Inst. Pêches Marit. 49: 35-66.

Rideout R., Tomkiewicz J. 2011. Skipped spawning in fishes: More common than you might think. Mar. Coast. Fish. 3: 176-189.

Schiavone R., Zilli L., Storelli C., Vilella S. 2012. Changes in hormonal profile, gonads and sperm quality of Argyrosomus regius (Pisces, Sciaenidae) during the first sexual differentiation and maturation. Theriogenology 77: 888-898. PMid:22153261

Schulz R.W, França L.R., Lareyre J.-J., LeGac F., Chiarini-Garcia H., Nobrega R.H., Miura T. 2011. Spermatogenesis in fish. Gen. Comp. Endocrinol. 165: 390-411. PMid:19348807

Taranger G.L., Carrillo M., Schulz R.W., Pascal F., Zanuy S., Felip A., Weltzien F.-A., Dufour S., Karlsen Ø., Norberg B., Andersson E., Hansen T. 2010. Control of puberty in farmed fish. Gen. Comp. Endocrinol. 165: 483-515. PMid:19442666

Tixerant G. 1974. Contribuition à l'étude de la biologie du maigre ou courbine sur la côte Mauritanienne. Ph.D. thesis, Univ. d'Aix-Marseille, 123 pp. West G. 1990. Methods of assessing ovarian development in fishes: A review. Aust. J. Mar. Freshw. Res. 41: 199-222.

Wilson C.A., Nieland D.L. 1994. Reproductive biology of red drum, Sciaenops ocellatus, from the neritic waters of the northern Gulf of Mexico. Fish. Bull. 82: 841-850.



How to Cite

Prista N, Gordo L, Lino Costa J, Costa MJ, Jones C. Reproductive phase determination in male meagre (Argyrosomus regius, Sciaenidae): testis development and histologic corroboration of a gross anatomical scale. scimar [Internet]. 2014Mar.30 [cited 2023Oct.4];78(1):65-80. Available from:




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