Scientia Marina, Vol 70, No S2 (2006)

PCR-SSCP of the 16S rRNA gene, a simple methodology for species identification of fish eggs and larvae


https://doi.org/10.3989/scimar.2006.70s213

Eva Garcia-Vazquez
Departamento Biología Funcional, Universidad de Oviedo., Spain

Paula Alvarez
Fundación AZTI. Departamento de Recursos Pesqueros. Pasaia (Guipúzcoa), Spain

Placida Lopes
Instituto de Investigaçao das Pescas e do Mar., Portugal

Nikoletta Karaiskou
Department of Genetics, Development and Molecular Biology, School of Biology, Aristotle University of Thessaloniki, Greece

Juliana Perez
Departamento Biología Funcional, Universidad de Oviedo., Spain

Ana Teia
Instituto de Investigaçao das Pescas e do Mar., Portugal

José L. Martinez
Departamento Biología Funcional, Universidad de Oviedo., Spain

Laurentina Gomes
Instituto de Investigaçao das Pescas e do Mar., Portugal

Costas Triantaphyllidis
Department of Genetics, Development and Molecular Biology, School of Biology, Aristotle University of Thessaloniki, Greece

Abstract


Patterns of the 16S rRNA gene obtained in 8 and 12% acrylamide gels by the SSCP (Single Strand Conformation Polymorphism) method were different for various marine fish species (Macrorhamphosus scolopax, Scomber scombrus, Lepidorhombus boscii, L. whiffiagonis, Trachurus trachurus, T. mediterraneus, Molva molva, Merluccius merluccius). SSCP patterns of this gene were employed to successfully identify formaldehyde-fixed eggs of different species (Merluccius merluccius, Scomber scombrus, Macrorhamphosus scolopax and L. whiffiagonis) in plankton samples. The advantages of SSCPs in comparison with current genetic methods of egg identification are based on their technical simplicity and low price. The application of the PCR-SSCP methodology is proposed for routine genetic analyses in plankton surveys.

Keywords


fish species; genetic markers; ichthyoplankton; mitochondrial genes; PCR-SSCP

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