Scientia Marina, Vol 79, No 1 (2015)

Growth rate variation of the stalked barnacle Pollicipes pollicipes (Crustacea: Cirripedia) using calcein as a chemical marker


https://doi.org/10.3989/scimar.04135.08B

David Jacinto
MARE, Marine and Environmental Sciences Center, Laboratório de Ciências do Mar, Universidade de Évora - Centro de Oceanografia, Universidade de Évora, Portugal

Nélia Penteado
MARE, Marine and Environmental Sciences Center, Laboratório de Ciências do Mar, Universidade de Évora - Centro de Oceanografia, Universidade de Évora, Portugal

Diana Pereira
MARE, Marine and Environmental Sciences Center, Laboratório de Ciências do Mar, Universidade de Évora - Centro de Oceanografia, Universidade de Évora, Portugal

Alina Sousa
MARE, Marine and Environmental Sciences Center, Laboratório de Ciências do Mar, Universidade de Évora - Centro de Oceanografia, Universidade de Évora, Portugal

Teresa Cruz
MARE, Marine and Environmental Sciences Center, Laboratório de Ciências do Mar, Universidade de Évora - Centro de Oceanografia, Universidade de Évora - Departamento de Biologia, Escola de Ciências e Tecnologia, Universidade de Évora, Portugal

Abstract


This study describes the use of calcein as a chemical tagging methodology to estimate growth rate variation of the stalked barnacle Pollicipes pollicipes, an ecologically important intertidal species and economic resource, in SW Portugal. Calcein tagging had a high success rate (94%) in marking both juvenile and adult barnacles for a period of 2.5 months, providing a valuable method for obtaining reliable data in growth studies of P. pollicipes. Growth rate decreased with barnacle size and was highly variable amongst individuals, particularly in smaller barnacles. No effect of shore level on barnacle growth was detected. Growth rates were higher in smaller juvenile barnacles, peaking at a 1.1-mm monthly increment in rostro-carinal length (RC) for individuals with RC=5 mm, and decreased with barnacle size (monthly growth rates of 0.5 mm for adult barnacles with RC~12.5 mm). Growth rates observed in adults with commercial interest (RC ≥ 18 mm) was < 0.25 mm per month. The advantages of tagging P. pollicipes with calcein were the possibility of mass marking individual barnacles of different size cohorts within a short period (less than 1 day of manipulation); and reduced time of fieldwork, which is very important because this species inhabits very exposed rocky shores.

Keywords


stalked barnacle; Pollicipes; growth rate; calcein; tagging; intertidal; Portugal

Full Text:


HTML PDF XML

References


Anderson D.T. 1994. Barnacles: structure, function, development and evolution. Chapman and Hall, London, 357 pp.

Bald J., Borja A., Muxika I. 2006. A system dynamics model for the management of the gooseneck barnacle (Pollicipes pollicipes) in the marine reserve of Gaztelugatxe (Northern Spain). Ecol. Modell. 194: 306-315. http://dx.doi.org/10.1016/j.ecolmodel.2005.10.024

Barnes M. 1996. Pedunculate cirripedes of the genus Pollicipes. Oceanogr. Mar. Biol. Annu. Rev. 34: 303-394.

Boukaici M., Bergayou H., Kaaya A., et al. 2012. Pollicipes pollicipes (Gmelin, 1789) (Cirripède, Lepadomorphe): étude de la croissance et de la dynamique des populations dans la région de Mirleft (sud ouest Marocain). Crustaceana. 85: 1073-1097. http://dx.doi.org/10.1163/156854012X651259

Cardoso A., Yule A. 1995. Aspects of the reproductive biology of Pollicipes pollicipes (Cirripedia: Lepadomorpha) from the southwest coast of Portugal. Netherlands J. Aquat. Ecol. 29: 391–396. http://dx.doi.org/10.1007/BF02084238

Chaffee J., Lewis C. 1988. Pedunculate barnacle stalk growth. J. Exp. Mar. Bio. Ecol. 124: 145-162. http://dx.doi.org/10.1016/0022-0981(88)90169-4

Cruz T. 1993. Growth of Pollicipes pollicipes (Gmelin, 1790) (Cirripedia, Lepadomorpha) on the SW coast of Portugal. Crustaceana. 65: 151-158. http://dx.doi.org/10.1163/156854093X00522

Cruz T. 2000. Biologia e ecologia do percebe, Pollicipes pollicipes (Gmelin, 1790), no litoral sudoeste português. PhD thesis, Univ. Évora, 328 pp.

Cruz T., Araújo J. 1999. Reproductive Patterns of Pollicipes pollicipes (Cirripedia: Scalpellomorpha) on the Southwestern Coast of Portugal. J. Crustac. Biol. 19: 260-267. http://dx.doi.org/10.2307/1549232

Cruz T., Castro J.J., Hawkins S.J. 2010. Recruitment, growth and population size structure of Pollicipes pollicipes in SW Portugal. J. Exp. Mar. Biol. Ecol. 392: 200-209. http://dx.doi.org/10.1016/j.jembe.2010.04.020

Ebert T.A., Russell M.P., Gamba G., et al. 2008. Growth, survival, and longevity estimates for the rock-boring sea urchin Echinometra lucunter lucunter (Echinodermata, Echinoidea) in Bermuda. Bull. Mar. Sci. 82: 381-403.

Frenkel V., Kindschi G., Zohar Y. 2002. Noninvasive, mass marking of fish by immersion in calcein: evaluation of fish size and ultrasound exposure on mark endurance. Aquaculture. 214: 169-183. http://dx.doi.org/10.1016/S0044-8486(02)00135-7

Helms A.R. 2004. Living on the edge: juvenile recruitment and growth of the gooseneck barnacle Pollicipes polymerus. M.Sc. Thesis, Univ. Oregon, 107 pp.

Hoffman D. 1984. Size-frequency distribution patterns of the juve- nile stages of the pendunculate barnacle, Pollicipes polymerus Sowerby, 1833 (Cirripedia, Lepadomorpha). Crustaceana. 46: 295-299. http://dx.doi.org/10.1163/156854084X00216

Jacinto D., Cruz T., Silva T., et al. 2010. Stalked barnacle (Pollicipes pollicipes) harvesting in the Berlengas Nature Reserve, Portugal: temporal variation and validation of logbook data. ICES J. Mar. Sci. 67: 19-25. http://dx.doi.org/10.1093/icesjms/fsp226

Kaehler S., McQuaid C. 1999. Use of the fluorochrome calcein as an in situ growth marker in the brown mussel Perna perna. Mar. Biol. 133: 455-460. http://dx.doi.org/10.1007/s002270050485

Kilada R., Sainte-Marie B., Rochette R., et al. 2012. Direct determination of age in shrimps, crabs, and lobsters. Can. J. Fish. Aquat. Sci. 69: 1728-1733. http://dx.doi.org/10.1139/cjfas-2012-0254

Lambert G., Lambert C. 1996. Spicule formation in the New Zealand ascidian Pyura pachydermatina (Chordata, Ascidiacea). Connect. Tissue Res. 34: 263-269. http://dx.doi.org/10.3109/03008209609005270 PMid:9084635

Leips J., Baril C., Rodd F., et al. 2001. The suitability of calcein to mark poeciliid fish and a new method of detection. Trans. Am. Fish. Soc. 130: 501-507. http://dx.doi.org/10.1577/1548-8659(2001)130<0501:TSOCTM>2.0.CO;2

Molares J., Freire J. 2003. Development and perspectives for community-based management of the goose barnacle (Pollicipes pollicipes) fisheries in Galicia (NW Spain). Fish. Res. 65: 485-492. http://dx.doi.org/10.1016/j.fishres.2003.09.034

Moran A. 2000. Calcein as a marker in experimental studies newlyhatched gastropods. Mar. Biol. 137: 893-898. http://dx.doi.org/10.1007/s002270000390

Page H. 1986. Differences in population structure and growth rate of the stalked barnacle Pollicipes polymerus between a rocky headland and an offshore oil platform. Mar. Ecol. Prog. Ser. 29: 157-164. http://dx.doi.org/10.3354/meps029157

Parada J., Outeiral R., Iglesias E., et al. 2012. Assessment of goose barnacle (Pollicipes pollicipes Gmelin, 1789) stocks in management plans: design of a sampling program based on the harvesters' experience. ICES J. Mar. Sci. 69: 1840-1849. http://dx.doi.org/10.1093/icesjms/fss157

Phillips N. 2005. Growth of filter-feeding benthic invertebrates from a region with variable upwelling intensity. Mar. Ecol. Prog. Ser. 295: 79-89. http://dx.doi.org/10.3354/meps295079

Russell M.P., Urbaniak L.M. 2004. Does calcein affect estimates of growth rates in sea urchins? In: Heinzeller T., Nebelsick J.H. (eds), Echinoderms: Munchen Proceedings of the 11th International Echinoderm Conference, 6-10 October 2003, Munich, Germany. Taylor & Francis, pp. 53-57. http://dx.doi.org/10.1201/9780203970881.ch10

Sousa A., Jacinto D., Penteado N., et al. 2013. Patterns of distribution and abundance of the stalked barnacle (Pollicipes pollicipes) in the central and southwest coast of continental Portugal. J. Sea Res. 83: 187-194. http://dx.doi.org/10.1016/j.seares.2013.04.005

Van der Geest M., van Gils J.A., van der Meer J., et al. 2011. Suitability of calcein as an in situ growth marker in burrowing bivalves. J. Exp. Mar. Bio. Ecol. 399: 1-7. http://dx.doi.org/10.1016/j.jembe.2011.01.003

Wilson C.A., Beckman D.W., Dean J.M. 1987. Calcein as a fluores- cent marker of otoliths of larval and juvenile fish. Trans. Am. Fish. Soc. 116: 668-670. http://dx.doi.org/10.1577/1548-8659(1987)116<668:CAAFMO>2.0.CO;2

Zuur A., Ieno E., Smith G. 2007. Analysing ecological data. Springer, New York, 672 pp. http://dx.doi.org/10.1007/978-0-387-45972-1




Copyright (c) 2015 Consejo Superior de Investigaciones Científicas (CSIC)

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.


Contact us scimar@icm.csic.es

Technical support soporte.tecnico.revistas@csic.es