Scientia Marina, Vol 74, No 2 (2010)

Effects of copper on the physiological responses of the commercial crab Lithodes santolla (Decapoda: Anomura) larvae


https://doi.org/10.3989/scimar.2010.74n2215

Oscar Antonio Amin
Centro Austral de Investigaciones Científicas (CADIC – CONICET), Argentina

Laura Inés Comoglio
Centro Austral de Investigaciones Científicas (CADIC – CONICET), Argentina

Abstract


Effects of copper toxicity on zoea I of Lithodes santolla (Decapoda: Anomura) were analysed. The 96-h LC50 was estimated, resulting in 298.5 µg L-1. Groups of larvae were exposed to sublethal concentrations (40, 80 and 160 µg L-1) for 96 h. Oxygen consumption, ammonia excretion, O:N atomic ratio, lipid peroxidation (LPO) and body water content were measured. Oxygen consumption of treated groups (mean 46.92 ± 8.03 µg-atom O2 h-1 mg-1) did not differ significantly with control. Ammonia excretion decreased by 60% at higher Cu concentration (1.61 ± 0.65 µg-atom N-NH3 h-1 mg-1), leading to a 117% increase in the O:N ratio. LPO values during the exposure time were higher in all treatments than in the controls. The water content was significantly higher in treatments than in controls. The highest concentration assayed, which represents about 50% of 96-h LC50, had evident effects on the parameters analysed. The values of copper in water reported for the coastal zone of Ushuaia bay exceed the value established by the United States Environmental Protection Agency (USEPA) for ambient water quality criteria. Therefore, the results obtained in the present study are a contribution to the study of potential effects of copper as a common stressor in the first larval stage of this commercial species of the Beagle Channel.

Keywords


crustacean larvae; oxygen consumption; ammonia excretion; O:N atomic ratio; lipid peroxidation; copper

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References


Amin, O.A., S. Andrade, L. Ferrer, L.I. Comoglio, J. Marcovecchio and N. San Román. – 1997. Use of macroalgae as bioindicator of heavy metals concentration in coastal zone of Beagle Channel, Tierra del Fuego, Argentina. Proc. Int. Symp. Cold Region Dev., 337-340.

Amin, O., E.M. Rodríguez, M. Hernando, L.I. Comoglio, L.S. López and D.A. Medesani. – 1998. Effects of lead and cadmium on hatching of the Southern King Crab Lithodes santolla (Decapoda, Anomura). Invertebr. Reprod. Dev., 33: 81-85.

Amin, O., L. Comoglio and E. Rodríguez. – 2003. Toxicity of Cadmium, Lead, and Zinc to larval stages of Lithodes santolla (Decapoda, Anomura). Bull. Environ. Contam. Toxicol., 71: 527-534. doi:10.1007/s00128-003-8611-9 PMid:14567579

American Public Health Association (APHA), American Water Works Association (AWWA) and Water Pollution Control Federation (WPCF). – 1995. Standard methods for the examination of water and wastewater. 19th Edition APHA, Washington D.C.

Barata, C., I. Varo, J.C. Navarro, S. Arun and C. Porte. – 2005. Antioxidant enzyme activities and lipid peroxidation in the freshwater cladoceran Daphnia magna exposed to redox cycling compounds. Comp. Biochem. Physiol. C, 140: 175-186.

Beuge, J.A. and S.D. Aust. – 1972. Microsomal Lipoperoxidation. Methods Enzymol., 52: 302-307.

Blanchard, J. and M. Grosell. – 2006. Copper toxicity across salinities from freshwater to seawater in the euryhaline fish Fundulus heteroclitus: Is copper an ionoregulatory toxicant in high salinities?. Aquat. Toxicol., 80: 131-139. doi:10.1016/j.aquatox.2006.08.001 PMid:16996624

Brouwner, M. and T.H. Brouwner. – 1998. Biochemical defense mechanisms against copper-induced oxidative damage in the blue crab, Callinectes sapidus. Arch. Biochem. Biophys., 351: 257-264. doi:10.1006/abbi.1997.0568 PMid:9514663

Cheng, X.X., C.Y. Lu and Y. Ye. – 2009. Effect of Cd and Zn on oxygen consumption and ammonia excretion in sipuncula (Phascolosoma sculenta). Ecotoxicol. Environ. Saf., 72: 507-515. doi:10.1016/j.ecoenv.2007.11.019 PMid:18272221

Comoglio, L.I. and J.H. Vinuesa. – 1991. Larval culture under laboratory conditions of southern king crab Lithodes santolla and the false southern king crab Paralomis granulosa. Eur. Aquac. Soc., Special Publication 15: 349-351.

Correia, A.D., D.R. Livingstone and M.H. Costa. – 2002. Effects of water-born copper on metallothionein and lipid peroxidation in the marine amphipod Gammarus lacusta. Mar. Environ. Res., 54: 357-360. doi:10.1016/S0141-1136(02)00114-9

Daniel, W.W. – 1978. Applied nonparametric statistics. Ed. Houghton Mifflin Company, Boston.

Depledge, M.H. and P.S Rainbow. – 1990. Models of regulation and accumulation of trace metals in marine invertebrates. Comp. Biochem. Physiol. C, 97(1): 1-7. doi:10.1016/0742-8413(90)90163-4

Espina, S. and R.C. Vanegas Perez. – 2006. Ecotoxicología y Contaminación, In: A.V. Botello, J. Rendón-von Osten, G. Gold-Bouchot and C. Agraz-Hernández (eds.), Golfo de México, Contaminación e Impacto Ambiental: Diagnóstico y Tendencias. EPOMEX Serie Científica 5, pp. 79-120. Universidad Autónoma de México.

Ferrer, L.D., J.S. Andrade, E.T. Contardi, R.O. Asteasuaian and J.E. Marcovecchio. – 2003. Copper and zinc concentrations in Bahía Blanca Estuary (Argentina), and their acute lethal effects on larvae of the crab Chasmagnathus granulata. Chem. Speciat. Biovailab., 15: 7-14. doi:10.3184/095422903782775271

Fingerman, M., M. Devi, P.S. Reddy and R. Katyayani. – 1996. Impact of heavy metal exposure on the nervous system and endocrine-mediated processes in crustaceans. Zool. Stud., 35: 1-8.

Finney, D.J. – 1971. Probit analysis. 3rd edition. Cambridge University Press, Cambridge.

Geracitano, L., J.M. Montserrat and A. Bianchini. – 2002. Physiological and antioxidant enzyme responses to acute and chronic exposure of Laeonereis acuta (Polychaeta, Nereididae) to copper. J. Exp. Mar. Biol. Ecol., 277: 145-156. doi:10.1016/S0022-0981(02)00306-4

Giarratano, E., L. Comoglio and O.A. Amin. – 2007. Heavy metal toxicity in Exosphaeroma gigas (Crustacea, Isopoda) from the coastal zone of Beagle Channel. Ecotoxicol. Environ. Saf., 68(3): 451-462. doi:10.1016/j.ecoenv.2006.11.008 PMid:17223192

Knops, M., R. Altenburger and H. Segner. – 2001. Alterations of physiological energetics, growth and reproduction of Daphnia magna under toxicant stress. Aquat. Toxicol., 53: 79-90. doi:10.1016/S0166-445X(00)00170-3

López Greco, L.S., M.V. Sánchez, G.L. Nicoloso, D.A. Medesani and E.M. Rodríguez. – 2001. Toxicity of cadmium and copper on larval and juvenile stages of the estuarine crab Chasmagnathus granulata (Brachyura, Grapsidae). Arch. Environ. Contam. Toxicol., 41: 333-338. doi:10.1007/s002440010256 PMid:11503070

Lovrich, G.A. – 1997. La pesquería mixta de las centollas Lithodes santolla y Paralomis granulosa (Anomura: Lithodidae) en Tierra del Fuego, Argentina. Invest. Mar., 25: 41-57.

Marcovecchio, J.E. – 2000. Land-based sources and activities affecting the marine environment at the Upper Southwestern Atlantic Ocean: an overview. UNEP RSRS, 170: 67 pp.

Markwell, M.A.K., S.M. Hass, L.L. Bieber and N.E. Tolbert. – 1978. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal. Biochem., 87: 206-210. doi:10.1016/0003-2697(78)90586-9

Mayzaud, P. and R.J. Conover. – 1988. O:N atomic ratio as a tool to describe zooplankton metabolism. Mar. Ecol. Prog. Ser., 45: 289-302. doi:10.3354/meps045289

Munshi, A.B., S.Y. Quan and S.J. Li. – 1996. Acute toxicity of copper, cadmium and copper-cadmium mixture to the larvae of the shrimp Penaeus monodon. Pak. J. Sci. Res., 39: 68-71.

Newman, M.C. and M.G. Heagler. – 1991. Allometry of metal bioaccumulation and toxicity. In: M.C. Newman and A.W. McIntosh (eds.), Metal Ecotoxicology. Lewis, Boca Raton, FL, USA.

Ramachandran, S., T.R. Patel and M.H. Colbo. – 1997. Effect of copper and cadmium on three Malaysian tropical estuarine invertebrate larvae. Ecotoxicol. Environ. Saf., 36: 183-188. doi:10.1006/eesa.1996.1508 PMid:9126437

Rikans, L.E. and K.R. Hornbrook. – 1997. Lipid peroxidation, antioxidant protection and aging. Biochim. Biophys Acta, 1362: 116–127. PMid:9540842

Ringwood, A.H., M.J. Hameedi, R.F. Lee, M. Brouwer, E.C. Peters, G.I. Scott, S.N. Luoma, R.T. DiGiulio. – 1999. Bivalve Biomarker Workshop: overview and discussion group summaries. Biomarkers, 4: 391-399. doi:10.1080/135475099230561

Scelzo, M.A. – 1997. Toxicidad del cobre en larvas nauplii del camarón comercial Artemesia longinaris Bate (Crustacea, Decapoda, Penaeidae). Invest. Mar., 25: 177-185.

Soegianto, A., M. Charmantier-Daures, J.P. Trilles and G. Charmantier.– 1999. Impact of cadmium on the structure of gills and epipodites of the shrimp Penaeus japonicus (Crustacea: Decapoda). Aquat. Living Resour., 12(1): 57-70. doi:10.1016/S0990-7440(99)80015-1

Sokal, R.R. and F.J. Rohlf. – 1981. Biometry. W.H. Freeman and Company, New York, USA.

Stohs, S.J. and D. Bagchi. – 1995. Oxidative mechanisms in the toxicity of metal ions. Free Radic. Biol. Med., 18(2): 321-336. doi:10.1016/0891-5849(94)00159-H

Strickland, J.D.H. and T.R. Parsons. – 1972. A practical handbook of seawater analysis. Bull. Fish Res. Board Can., Bulletin 167, 2nd ed.

Taboada, G., G. Gaxiola, T. García, R. Pedroza, A. Sanchez, L.A. Soto and C. Rosas. 1998. Oxygen consupmtion and ammonia N-excretion related to protein requirements for growth of white shrimp, Penaeus vannammei (L.), juveniles. Aquac. Res., 29: 822-833. doi:10.1111/j.1365-2109.1998.tb01108.x

United Sates Environmental Protection Agency. – 1999. National Recommended Water Quality Criteria-Correction. Office of Water Epa 822-Z-99-001.

Viarengo, A., M. Lafaurie, G.P. Gabrielides, R. Fabbri, A. Marro and M. Romeo. – 2000. Critical evaluation of an intercalibration exercise undertaken in the framework of the MED POL. biomonitoring program. Mar. Environ. Res., 49: 1-18. doi:10.1016/S0141-1136(99)00045-8

Villar, C, J. Stripeikis, M. Tudino, L. d’Huicque, O. Troccoli and C. Bonetto. – 1999. Trace metal concentrations in coastal marshes of the lower Paraná River and the Río de la Plata estuary. Hydrobiol., 397: 187-195. doi:10.1023/A:1003730306880

Vosloo, A., W.J. Van Aardt and L.J. Mienie. – 2002. Sublethal effects of copper on the freshwater crab Potamonautes warreni. Comp. Biochem. Physiol. A, 133: 695-702. doi:10.1016/S1095-6433(02)00214-3

Wilson, R.W. and E.W. Taylor. – 1993. Differential responses to copper in Rainbow Trout (Oncorhynchus mykiss) acclimated to seawater and brackish water. J. Comp. Physiol. B, 163: 239-246. doi:10.1007/BF00261671

Zapata, V., L. López Greco and E. Rodríguez. – 2001. Effect of copper on hatching and development of larvae of the estuarine crab Chasmagnathus granulata (Decapoda, Brachyura). Environ. Toxicol. Chem., 20(7): 1579-1583. PMid:11434301




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