Identification and characterisation of the dominant <em>Pseudo-nitzschia</em> species (Bacillariophyceae) along the NE Spanish coast (Catalonia, NW Mediterranean)

Sonia Quijano-Scheggia; Esther Garcés; Nagore Sampedro; Kees van Lenning; Eva Flo; Karl Andree; José-Manuel Fortuño; Jordi Camp

doi:10.3989/scimar.2008.72n2343

Autores/as

Sonia Quijano-Scheggia Departament de Biologia Marina i Oceanografia, Institut de Ciències del Mar-CMIMA (CSIC)
Esther Garcés Departament de Biologia Marina i Oceanografia, Institut de Ciències del Mar-CMIMA (CSIC)
Nagore Sampedro Departament de Biologia Marina i Oceanografia, Institut de Ciències del Mar-CMIMA (CSIC)
Kees van Lenning Departament de Biologia Marina i Oceanografia, Institut de Ciències del Mar-CMIMA (CSIC)
Eva Flo Departament de Biologia Marina i Oceanografia, Institut de Ciències del Mar-CMIMA (CSIC)
Karl Andree Centre d’Aquicultura-IRTA
José-Manuel Fortuño Departament de Biologia Marina i Oceanografia, Institut de Ciències del Mar-CMIMA (CSIC)
Jordi Camp Departament de Biologia Marina i Oceanografia, Institut de Ciències del Mar-CMIMA (CSIC)

DOI:

https://doi.org/10.3989/scimar.2008.72n2343

Palabras clave:

diatomeas, Pseudo-nitzschia, proliferaciones algales nocivas, autoecología, ITS, morfología, pigmentos

Resumen

Se estudiaron especies potencialmente tóxicas de Pseudonitzschia en la costa española del Mediterráneo NO de enero 2005 a mayo 2006. Observaciones al microscopio electrónico revelan la presencia de Pseudo-nitzschia brasiliana, P. calliantha, P. delicatissima, P. fraudulenta, P. multistriata y P. pungens. Se aislaron varias cepas de las aguas costeras y fueron comparadas entre si mediante técnicas combinadas que incluyen microscopia óptica, electrónica, secuenciación del ITS-1, 5.8S e ITS-2 rDNA. Cultivos de las cepas aisladas fueron analizadas con HPLC para estudiar la posibilidad de discriminarlas por medio de un método quemotaxonómico simple. El género Pseudo-nitzschia muestra concentraciones celulares altas durante la mayor parte del año, pero la población inicia un declive brusco en todos los puntos de muestreos en el periodo entre abril y mayo. P. delicatissima y P. calliantha fueron encontradas en las estaciones del norte entre febrero y abril, y en las del sur entre agosto y noviembre. P. brasiliana y P. multistriata fueron detectadas sólo ocasionalmente en las estaciones del sur. Ninguna de las variables ambientales consideradas explica satisfactoriamente la distribución temporal y espacial de las especies de Pseudo-nitzschia en el área de estudio. Diferencias en la tasa de crecimiento y producción celular de las especies indica que los factores bióticos pueden jugar un papel importante en los patrones de distribución observados.

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Citas

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