Bloom dynamics of the genus Pseudo-nitzschia (Bacillariophyceae) in two coastal bays (NW Mediterranean Sea)


  • Sonia Quijano-Scheggia Departament de Biologia Marina i Oceanografia, Institut de Ciències del Mar-CMIMA, CSIC
  • Esther Garcés Departament de Biologia Marina i Oceanografia, Institut de Ciències del Mar-CMIMA, CSIC
  • Eva Flo Departament de Biologia Marina i Oceanografia, Institut de Ciències del Mar-CMIMA, CSIC
  • Margarita Fernandez-Tejedor IRTA, Institut de Recerca i Tecnologia Agroalimentaries-Centre d’Aqüicultura
  • Jorge Diogène IRTA, Institut de Recerca i Tecnologia Agroalimentaries-Centre d’Aqüicultura
  • Jordi Camp Departament de Biologia Marina i Oceanografia, Institut de Ciències del Mar-CMIMA, CSIC



Pseudo-nitzschia, bloom dynamics, phytoplankton community


The spatial and temporal variations in the composition of Pseudo-nitzschia during bloom events from August 2005 to February 2006 were characterised in two bays of the NW Mediterranean Sea (Alfacs and Fangar Bay) by means of scanning electron microscopy (SEM). The study provides detailed records of the Pseudo-nitzschia community at the species level and describes its relationship with both the surrounding environmental conditions and biotic factors such as the accompanying phytoplankton community. The size distributions of several species of Pseudo-nitzschia were monitored during the bloom events. These measurements may serve as indicators of the physiological status of the cells. The species observed in the two bays were Pseudo-nitzschia calliantha, P. delicatissima, P. fraudulenta, P. multistriata, and P. pungens. In Alfacs Bay, a mixed species bloom of P. calliantha and P. delicatissima began in late August 2005 and lasted 11 weeks. In Fangar Bay, the Pseudo-nitzschia bloom was limited to the period from early August to late September 2005 and comprised P. calliantha and P. delicatissima. Commonly, the proliferation of Pseudo-nitzschia was mono-specific or was accompanied by other diatoms. Two objectively defined groups were identified by the statistical analysis in Alfacs bay; the first was made up only of winter samples and the second of summer and autumn samples. The first group was defined by a high concentration of NO3¯ and low concentrations of NH4+, conditions associated with a high abundance of P. delicatissima and a low abundance of P. calliantha. The second group expressed the opposite characteristics. A succession of different blooming species of Pseudo-nitzschia lasting months in Alfacs Bay is described.


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Amato, A., W. Kooistra, J.H.L. Ghiron, D.G. Mann, T. Pröschold and M. Montresor. _ 2007. Reproductive isolation among sympatric cryptic species in marine diatoms. Protist. 158: 193-207. doi:10.1016/j.protis.2006.10.001

Bates, S.S. _ 2000. Domoic-acid-producing diatoms: another genus added! J. Phycol. 36: 978-983. doi:10.1046/j.1529-8817.2000.03661.x

Bates, S.S., D.L. Garrison and R. Horner. _ 1998. Bloom dynamics and physiology of domoic-acid-producing Pseudo-nitzschia species. In: D. Anderson, A. Cembella and G.M Hallegraef. (eds.), Physiological ecology of harmful algal blooms, pp. 267- 292. Springer Verlag, Berlin.

Camp, J. _ 1994. Aproximaciones a la dinámica ecológica de una bahía estuárica mediterránea. PhD thesis, Univ. Barcelona

Camp, J. and M. Delgado. _ 1987. Hidrografía de las bahías del delta del Ebro. Inv. Pesq., 51: 351-369.

Cerino, F., L. Orsini, D. Sarno, C. Dell’Aversano, L. Tartaglione and A. Zingone. _ 2005. The alternation of different morphotypes in the seasonal cycle of the toxic diatom Pseudo-nitzschia galaxiae. Harmful Algae, 4: 33. doi:10.1016/j.hal.2003.10.005

Clark, K.R. and R.M. Warwick. _ 2001. Change in marine communities: an approach to statistical analysis and interpretation. PRIMER-E, Plymouth, U. K.

Cusack, C., R. Raine and J.W. Patching. _ 2004. Occurrence of species from the genus Pseudo-nitzschia Peragallo in Irish waters. Proc. Royal Irish Acad. 104B: 55-74. doi:10.3318/BIOE.2004.104.1.55

Chepurnov, V.A. and D.G. Mann. _ 2004. Auxosporulation of Licmophora communis (Bacillariophyta) and a review of mating systems and sexual reproduction in araphid pennate diatoms. Phycol. Res., 52: 1-12. doi:10.1111/j.1440-1835.2004.tb00309.x

Chepurnov, V.A., D.G. Mann and K. Sabbe. _ 2004. Experimental studies on sexual reproduction in diatoms. Int. Rev. Cyt., 237: 91-154. doi:10.1016/S0074-7696(04)37003-8

Chepurnov, V.A., D.G. Mann, K. Sabbe, K. Vannerum, G. Casteleyn, E. Verleyen, L. Peperzak and W. Vyverman. _ 2005. Sexual reproduction, mating system, chloroplast dynamics and abrupt cell size reduction in Pseudo-nitzschia pungens from the North Sea (Bacillariophyta). Eur. J. Phycol. 40: 379-395. doi:10.1080/09670260500254735

Cho, E.S., H.J. Hur, H.S. Byun, S.G. Lee, L.L. Rhodes, C.S. Jeong and J.G. Park. _ 2002. Monthly monitoring of domoic acid producer Pseudo-nitzschia multiseries (Hasle) Hasle using species-specific DNA probes and WGA lectins and abundance of Pseudo-nitzschia species (Bacillariophyceae) from Chinhae Bay, Korea. Bot. Mar., 45: 364-372. doi:10.1515/BOT.2002.037

Davidovich, N. and B. Bates. _ 1998. Sexual reproduction in the pennate diatoms Pseudo-nitzschia multiseries and P psedodelicatissima (Bacillariophycea). J. Phycol. 34: 126-137. doi:10.1046/j.1529-8817.1998.340126.x

Delgado, M. _ 1987. Fitoplancton de las bahías del Delta del Ebro. Inv. Pesq., 51: 517-548.

Drebes, G. _ 1977. Sexuality. In: E.D. Werner (ed.), The Biology of Diatoms (Botanical monographs 13), pp. 250-283. Blackwell Oxford.

Elandaloussi, L., R. Venail, S. Quijano-Scheggia, M. Fernández-Tejedor, E. Mallat, J. Diogene, E. Garcés, J. Camp and K. Andree. _ 2006. Molecular tools for the identification of Pseudo-nitzschia calliantha and P. delicassima in the Ebre Delta, Spain, 12 th Int. Conf. Harmful Algae, Copenhagen, Denmark.

Fernández, L., A. Ocaña, R. Fernández and I. Márquez. _ 2004. Control y seguimiento de biotoxinas marinas en la costa andaluza. Episodios detectados durante 2001 y 2002. In: M.F.C. Norte Martín, J.J. La Laguna (ed.), Actas VII Reunión Ibérica fitoplancton tóxico y biotoxinas, La Laguna. 109-120.

Grasshoff, K., M. Ehrrardt and K. Kremling. _ 1983. Methods of sea water analysis. Chemie, Germany.

Hasle, G.R. _ 1995. Pseudo-nitzschia pungens and P. multiseries (Bacillariophyceae): nomenclatural history, morphology, and distribution. J. Phycol., 31: 428-435. doi:10.1111/j.0022-3646.1995.00428.x

Hasle, G.R. _ 2002. Are most of the domoic acid-producing Pseudonitzschia, bloom dynamics, phytoplankton community species of the diatom genus Pseudo-nitzschia cosmopolites? Harmful Algae, 1: 137-146. doi:10.1016/S1568-9883(02)00014-8

Hasle, G.R., C.B. Lange and E.E. Syvertsen. _ 1996. A review of Pseudo-nitzschia, with special reference to the Skagerrak, North Atlantic, and adjacent waters. Helgol. Meeresunters, 50: 131-175. doi:10.1007/BF02367149

Holm-Hansen, O., C.J. Lorenzen, R.W. Holmes and J.D.H. Strickland. _ 1965. Fluorometric determination of chlorophyll. J. Cons., 30: 3-15.

Ifremer. _ 2003. Bulletin de la surveillance, Document de programmation Ifremer: 44.

Justic, D., N.N. Rabalais, R.E. Turner and Q. Dortch. _ 1995. Changes in nutrient structure of river-dominated coastal waters: Stoichiometric nutrient balance and its consequences. Estuar Coast Shelf. Sci., 40: 339-356. doi:10.1016/S0272-7714(05)80014-9

Kaczmarska, I., M.M. LeGresley, J.L. Martin and J. Ehramn. _ 2005. Diversity of the diatom genus Pseudo-nitzschia Peragallo in the Quoddy Region of the Bay of Fundy, Canada. Harmful Algae, 4: 1-19. doi:10.1016/j.hal.2003.07.001

Kaczmarska, I., J.L. Martin, J.M. Ehrman and M.M. LeGresley. _ 2007. Pseudo-nitzschia species population dynamics in the Quoddy Region, Bay of Fundy. Harmful Algae, 6: 861-874. doi:10.1016/j.hal.2007.05.001

Lundholm, N., G.R. Hasle, G.A. Fryxell and P.E. Hargraves. _ 2002. Morphology, phylogeny and taxonomy of species within the Pseudo-nitzschia americana complex (Bacillariophyceae) with descriptions of two new species, Pseudo-nitzschia brasiliana and Pseudo-nitzschia linea. Phycologia, 41: 480-497.

Lundholm, N., Ø. Moestrup, G.R. Hasle and K. Hoef-Emden. _ 2003. A study of the Pseudo-nitzschia pseudodelicatissima/cuspidata complex (Bacillariophyceae): what is P. pseudodelicatissima? J. Phycol. 39: 797-813. doi:10.1046/j.1529-8817.2003.02031.x

Lundholm, N., Ø. Moestrup, Y. Kotaki, K. Hoef-Emden, C. Scholin and P. Miller. _ 2006. Inter- and intraspecific variation of the Pseudo-nitzschia delicatissima complex (Bacillariophyceae) illustrated by rRNA probes, morphological data and phylogenetic analyses. J. Phycol. 42: 464-481. doi:10.1111/j.1529-8817.2006.00211.x

Mann, D.G. _ 1988. Why didn’t Lund see sex in Asterionella? A discussion of the diatom life cycle in nature. In: F.E. Round (ed.), Algae and the Aquatic Environment, pp. 383-412. Biopress, Bristol.

Mann, D.G. _ 1994. Auxospore formation, reproductive plasticity and cell structure in Navicula ulvacea and the resurrection of the genus Dickieia (Bacillariophyta). Eur. J. Phycol., 29: 141-157. doi:10.1080/09670269400650591

Mann, D.G. _ 2002. Life cycles in diatoms. In: E. Garcés, A. Zingone, M. Montresor, B. Reguera and B. Dale (eds.), Lifehab Workshop: Life history of microalgal species causing harmful algal blooms. Comm. Eur. Community.

Mann, D.G. and V.A. Chepurnov. _ 2005. Auxosporulation, mating system, and reproductive isolation in Neidium (Bacillariophyta). Phycologia, 44.

Mann, D.G., V.A. Chepurnov and S.J.M. Droop. _ 1999. Sexuality, incompatibility, size variation, and preferential polyandry in natural populations and clones of Sellaphora pupula (Bacillariophyceae). J. Phycol., 35: 152-170. doi:10.1046/j.1529-8817.1999.3510152.x

Mann, D.G., V.A. Chepurnov and M. Idei. _ 2003. Mating system, sexual reprodution, and auxosporulation in the anomalous raphid diatom Eunotia (Bacillariophyta). J. Phycol., 39: 1067-1084. doi:10.1111/j.0022-3646.2003.03-011.x

Miller, P. and C.A. Scholin. _ 1998. Identification and enumeration of cultured and wild Pseudo-nitzschia (Bacillariophyceae) using species-specific LSU r RNA-targeted fluorescent probes and filter-based whole cell hybridization. J. Phycol., 34: 371-382. doi:10.1046/j.1529-8817.1998.340371.x

Miller, P.E. and C.A. Scholin. _ 2000. On detection of Pseudonitzschia (Bacillariophyceae) species using whole cell hybridization: sample fixation and stability. J. Phycol., 36: 238-250. doi:10.1046/j.1529-8817.2000.99041.x

Moestrup, Ø., G.A. Codd, M. Elbraechter, M.A. Faust, S. Fraga, Y. Fukuyo, G. Cronberg, Y. Halim, F.J.R. Taylor and A. Zingone. _ 2004. Taxonomic Reference List of Toxic Algae, I. O. C. UNESCO.01/12/2007

Orsini, L., G. Procaccini, D. Sarno and M. Montresor. _ 2004. Multiple rDNA ITS-types within the diatom Pseudo-nitzschia delicatissima (Bacillariophyceae) and their relative abundance across a spring bloom in the Gulf of Naples. Mar. Ecol. Prog. Ser., 271: 87-89. doi:10.3354/meps271087

Orsini, L., D. Sarno, G. Procaccini, R. Poletti, J. Dahlmann and M. Montresor. _ 2002. Toxic Pseudo-nitzschia multistriata (Bacillariophyceae) from the Gulf of Naples: morphology, toxin analysis and phylogenetic relationships with other Pseudo-nitzschia species. Eur. J. Phycol., 37: 247-257. doi:10.1017/S0967026202003608

Parsons, M.L., Q. Dortch and R.E. Turner. _ 2002. Sedimentological evidence of an increase in Pseudo-nitzschia (Bacillariophyceae) abundance in response to coastal eutrophication. Limnol. Oceanogr., 47: 551-558.

Parsons, M.L., C.A. Scholin, P.E. Miller, G.J. Doucette, C.L. Powell, G.A. Fryxell, Q. Dortch and T.M. Soniat. _ 1999. Pseudonitzschia species (Bacillariophyceae) in Louisiana coastal waters: Molecular probe field trials, genetic variability, and domoic acid analyses. J. Phycol., 35: 1368-1378. doi:10.1046/j.1529-8817.1999.3561368.x

Prat, N., I. Muñoz, J. Camp, F.A. Comin, J.R. Lucena, J. Romero and M. Vidal. _ 1988. Seasonal changes in particulate organic carbon and nitrogen in the river and drainage channels of the Ebro Delta (N.E. Spain). Verh. der Internat. Verin. Limnol., 23: 1344-1349.

Priisholm, K., Ø. Moestrup and N. Lundholm. _ 2002. Taxonomic notes on the marine diatom genus Pseudo-nitzschia in the Andaman Sea near the island of Phuket, Thailand, with a description of Pseudo-nitzschia micropora sp nov. Diatom Res., 17: 153-175.

Quijano-Scheggia, S., E. Garcés, N. Sampedro, K. van Lenning, E. Flo, K. Andree, J.M. Fortuño and J. Camp. _ 2008. Identification and characterization of the dominant Pseudo-nitzschia species (Bacillariophyceae) along the NE Spanish coast (Catalonia, NW Mediterranean). Sci. Mar., 72 (2): 343-359.

Round, F.E., R.M. Crawford and D.G. Mann. _ 1990. The diatoms. Biology and morphology of the genera. Cambridge Univ. Press, Cambridge.

Spatharis, S., G. Tsirtsis, D.B. Danielidis, T.D. Chi and D. Mouillot. _ 2007. Effects of pulsed nutrient inputs on phytoplankton assemblage structure and blooms in an enclosed coastal area. Estuar. Coast. Shelf Sci., 73: 807-815. doi:10.1016/j.ecss.2007.03.016

Throndsen, J. _ 1995. Estimating cell numbers. In: G.M. Hallegraeff, D.M. Anderson and A.D. Cembella (eds.), Manual on Harmful Marine Microalgae. IOC Manuals and Guides UNESCO, pp. 63-80, Paris.

Tomas, C.R. _ 1997. Identifying marine phytoplankton. Academic Press, San Diego.

Trainer, V.L. and B.H. Hickey. _ 2003. The challenges of forecasting and managing toxigenic Pseudo-nitzschia blooms on the US west coast. In: N. Valette-Silver and D. Scavia (ed.), Ecol. Forecasting: New tools Coast. Mar. Ecosyst. Managet. NOA Tech. Memo. NOS NCOS 1. pp. 55-59.

Trainer, V.L., B.M. Hickey and R. Horner. _ 2002. Biological and physical dynamics of domoic acid production off the Washington coast. Limnol. Ocanogr., 47: 1438-1446.




How to Cite

Quijano-Scheggia S, Garcés E, Flo E, Fernandez-Tejedor M, Diogène J, Camp J. Bloom dynamics of the genus Pseudo-nitzschia (Bacillariophyceae) in two coastal bays (NW Mediterranean Sea). Sci. mar. [Internet]. 2008Sep.30 [cited 2024May26];72(3):577-90. Available from:




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