Identification of <em>Chattonella</em> (Raphidophyceae) species in long-term phytoplankton samples from Santa Giusta Lagoon, Italy

Daniela Stacca; Cecilia Teodora Satta; Silvia Casabianca; Antonella Penna; Bachisio Mario Padedda; Nicola Sechi; Antonella Lugliè

doi:10.3989/scimar.04292.09A

Autores/as

Daniela Stacca Dipartimento di Architettura, Design e Urbanistica, University of Sassari
Cecilia Teodora Satta Dipartimento di Architettura, Design e Urbanistica, University of Sassari
Silvia Casabianca Dipartimento di Scienze Biomolecolari, University of Urbino
Antonella Penna Dipartimento di Scienze Biomolecolari, University of Urbino
Bachisio Mario Padedda Dipartimento di Architettura, Design e Urbanistica, University of Sassari
Nicola Sechi Dipartimento di Architettura, Design e Urbanistica, University of Sassari
Antonella Lugliè Dipartimento di Architettura, Design e Urbanistica, University of Sassari

DOI:

https://doi.org/10.3989/scimar.04292.09A

Palabras clave:

genotipos de Chattonella subsalsa, ecosistemas de transición, proliferaciones algales nocivas, LTER-Italia, ITS-5.8S rDNA, LSU rDNA

Resumen

Se identificaron especies de Chattonella mediante la aplicación de técnicas moleculares en muestras naturales de fitoplancton. Las muestras fueron recogidas y fijadas durante las últimas dos décadas en una laguna litoral mediterránea (Laguna de Santa Giusta, Cerdeña, Italia). Al igual que otras rafidoficeas, las células de Chattonella no poseen teca y, por lo tanto, pierden su forma cuando se fijan lo que dificulta la identificación basada en características morfológicas. Con el uso de cebadores específicos a nivel de especie (OBTG-005-F, OBTG-027-R, OBTG-028-R) diseñados para la amplificación de la región ITS- 5.8S rDNA, se detectó la presencia de C. subsalsa en las muestras recogidas en periodos coincidentes con eventos de muerte de peces. A través del análisis de los cultivos celulares obtenidos de la misma laguna en el año 2013, se identificó la presencia, por segunda vez a nivel mundial, del recientemente descubierto genotipo Adriático de C. cf. subsalsa. Los resultados revelaron que los cebadores oBTG-005-F y oBTG-028-R amplifican este nuevo genotipo sólo cuando está presente individualmente. En este estudio se presentan datos relevantes para el conocimiento de los genotipos de C. subsalsa y sobre la presencia recurrente de proliferaciones de especies de Chatonella en un ecosistema de transición a través de la utilización de muestras recogidas durante los últimos veinte años y analizadas hoy en día.

Descargas

Los datos de descargas todavía no están disponibles.

Citas

Adachi M., Sako Y., Ishida Y. 1994. Restriction fragment length polymorphism of ribosomal DNA internal transcribed spacer and 5.8S regions in Japanese Alexandrium species (Dinophyceae). J. Phycol. 30: 857-863. http://dx.doi.org/10.1111/j.0022-3646.1994.00857.x

Attaran-Fariman G., Bolch C.J.S. 2014. Morphology and genetic affinities of a novel Chattonella isolate (Raphidophyceae) isolated from Iran's south coast (Oman Sea). Turk. J. Bot. 38: 156-168. http://dx.doi.org/10.3906/bot-1210-33

Band-Schmidt C.J., Morquecho L., Hernandez-Becerril D.U., et al. 2004. Raphidophyceans on the coasts of Mexico. Hydrobiologia 515: 79-89. http://dx.doi.org/10.1023/B:HYDR.0000027320.00977.8b

Band-Schmidt C.J., Martínez-López A., Bustillos-Guzmán J.J., et al. 2012. Morphology, biochemistry, and growth of raphidophyte strains from the Gulf of California. Hydrobiologia 693: 81-97. http://dx.doi.org/10.1007/s10750-012-1088-y

Ben Ali A., De Baere R., De Wachter R., et al. 2002. Evolutionary relationships among heterokont algae (the autotrophic stramenopiles) based on combined analyses of small and large subunit ribosomal RNA. Protist 153(2): 123-132. http://dx.doi.org/10.1078/1434-4610-00091 PMid:12125754

Biecheler B. 1936. Sur une Chloromonadine nouvelle d'eau saumatre Chattonella subsalsa n. gen., n. sp. Arch. Zool. Exp. Gen. 78: 79-83.

Bowers H., Tomas C., Tengs T., et al. 2006. Raphidophyceae [Chadefaud ex Silva] systematics and rapid identification: sequence analyses and real-time PCR assays. J. Phycol. 42: 1333-1348. http://dx.doi.org/10.1111/j.1529-8817.2006.00285.x PMid:20411032 PMCid:PMC2856949

Connell L.B. 2000. Nuclear ITS region of the alga Heterosigma akashiwo (Chromophyta: Raphidophyceae) is identical in isolates from Atlantic and Pacific basins. Mar. Biol. 136: 953-960. http://dx.doi.org/10.1007/s002270000314

Connell L. 2002. Rapid identification of marine algae (Raphidophyceae) using three-primer PCR amplification of nuclear internal transcribed spacer (ITS) regions from fresh and archived material. Phycologia 41(1): 15-21. http://dx.doi.org/10.2216/i0031-8884-41-1-15.1

Demura M., Noël M.H., Kasai F., et al. 2009. Taxonomic revision of Chattonella antiqua, C. marina and C. ovata (Raphidophyceae) based on their morphological characteristics and genetic diversity. Phycologia 48(6): 518-535. http://dx.doi.org/10.2216/08-98.1

Guillard R.R.L., Hargraves P.E. 1993. Stichochrysis immobilis is a diatom, not a chrysophyte. Phycologia 32(3): 234-236. http://dx.doi.org/10.2216/i0031-8884-32-3-234.1

Hall T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl. Acid. S. 41: 95-98.

Hallegraeff G.M., Hara Y. 2003. Taxonomy of harmful marine raphidophytes. In: Hallegraeff G.M., Anderson D.M., Cembella A.D. (eds), Manual on harmful marine microalgae. Monographs on Oceanographic Methodology. Methodology 11, Unesco Paris, pp. 511-522.

Hallegraeff G.M., Munday B., Baden D., et al. 1998. Chattonella marina (Raphidophyte) bloom associated with mortality of cultured bluefin tuna (Thunnus maccoyii) in south Australia. In: Reguera B., Blanco J., Fernandez M.L., et al. (eds), Harmful Algae. Xunta de Galicia and IOC-UNESCO, Vigo, Paris, pp. 93-96.

Imai I., Yamaguchi M., Hori Y. 2006. Eutrophication and occurrences of harmful algal blooms in the Seto Inland Sea. Plank. Benth. Res. 1: 71-84. http://dx.doi.org/10.3800/pbr.1.71

Imai I., Yamaguchi M. 2012. Life cycle, physiology, ecology and red tide occurrences of the fish-killing raphidophyte Chattonella. Harmful Algae 14: 46-70. http://dx.doi.org/10.1016/j.hal.2011.10.014

Jugnu R., Kripa V. 2009. Effect of Chattonella marina [(Subrahmanyan) Hara et Chihara 1982] bloom on the coastal fishery resources along Kerala coast, India. Indian J. Mar. Sci. 38: 77-88.

Katoh K., Misawa K., Kuma K., et al. 2002. MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Res. 30(14): 3059-3066. http://dx.doi.org/10.1093/nar/gkf436 PMid:12136088 PMCid:PMC135756

Kim S.Y., Seo K.S., Lee C.K., et al. 2007. Diurnal modification of a red tide causing organisms, Chattonella antiqua (Raphidophyceae) from Korea. Algae 22: 95-106. http://dx.doi.org/10.4490/ALGAE.2007.22.2.095

Klöpper S., John U., Zingone A., et al. 2013. Phylogeny and morphology of a Chattonella (Raphidophyceae) species from the Mediterranean Sea: what is C. subsalsa? Eur. J. Phycol. 48(1):79-92. http://dx.doi.org/10.1080/09670262.2013.771412

Lewitus A.J., Brock L.M., Burke M.K., et al. 2008. Lagoonal stormwater detention ponds as promoters of harmful algal blooms and eutrophication along the South Carolina coast. Harmful Algae 8: 60-65. http://dx.doi.org/10.1016/j.hal.2008.08.012

Lugliè A., Sechi N., Oggiano G., et al. 2002. Ecological assessment of Santa Giusta Lagoon (Sardinia, Italy). Ann. Chim-Rome 92: 239-247.

Marin III R., Scholin C. 2010. Sandwich Hybridization. In: Karlson B., Cusack C., Bresnan E. (eds), Microscopic and molecular methods for quantitative phytoplankton analysis. IOC Man Guides 55, Unesco Paris, pp 87-110.

Mikhail S.K. 2007. First monospecific bloom of the harmful raphidophyte Chattonella antiqua (Hada) Ono in Alexandria waters related to water quality and copepod grazing. Chem. Ecol. 23(5): 393-407. http://dx.doi.org/10.1080/02757540701587657

Penna A., Bertozzini E., Battocchi C., et al. 2007. Monitoring of HAB species in the Mediterranean Sea through molecular techniques. J. Plankton Res. 29: 19-38. http://dx.doi.org/10.1093/plankt/fbl053

Satta C.T., Anglès S., Garcès E., et al. 2014. Dinoflagellate cyst assemblages in surface sediments from three shallow Mediterranean Lagoons (Sardinia, North Western Mediterranean Sea). Estuar. Coasts 37: 646-663. http://dx.doi.org/10.1007/s12237-013-9705-1

Scholin C.A., Herzog M., Sogin M., et al. 1994. Identification of group and strain-specific genetic markers for globally distributed Alexandrium (Dinophyceae). 2. Sequence analysis of a fragment of the LSU rRNA gene. J. Phycol. 30(6): 999-1011. http://dx.doi.org/10.1111/j.0022-3646.1994.00999.x

Sechi N., Fiocca F., Sannio A., et al. 2001. Santa Giusta Lagoon (Sardinia): phytoplankton and nutrients before and after waste water diversion. J. Limnol. 60(2): 194-200. http://dx.doi.org/10.4081/jlimnol.2001.1.194

Stamatakis A. 2006. RAxML-VI-HPC: Maximum Likelihood-based Phylogenetic Analyses with Thousands of Taxa and Mixed Models. Bioinformatics 22(21): 2688-2690. http://dx.doi.org/10.1093/bioinformatics/btl446 PMid:16928733

Subrahmanyan R. 1954. On the life-history and ecology of Hornellia marina gen. et sp. nov., (Chloromonadineae), causing green discoloration of the sea and mortality among marine organisms off the Malabar Coast. Indian J. Fish. 1: 182-203.

Tomas C. 1998. Blooms of potentially harmful raphidophycean flagellates in Florida coastal waters. In: Reguera B., Blanco J., Fernandez M.L., et al. (eds), Harmful Algae. Xunta de Galicia and IOC-UNESCO, pp. 101-103.

Tseng C.K., Zhou M.J., Zou J.Z. 1993. Toxic phytoplankton studies in China. In: Smayda T.J., Shimizu Y. (eds), Toxic Phytoplankton Blooms in the Sea. Elsevier, New York, pp. 347-352.

Utermöhl H. 1958. Zur vervollkommung der quantitativen phytoplankton-methodik. Verkandlungen der Internationalen Vereinigung für Theoretische und Angewandte, Limnol. 9: 1-39.

Zhang Y., Fu F.-X., Whereat E., Coyne K.J., Hutchins D.A. 2006. Bottom-up controls on a mixed-species HAB assemblage: A comparison of sympatric Chattonella subsalsa and Heterosigma akashiwo (Raphidophyceae) isolates from the Delaware Inland Bays. Harmful Algae 5: 310-320. http://dx.doi.org/10.1016/j.hal.2005.09.001

Zingone A., Siano R., D'Alelio D., et al. 2006. Potentially toxic and harmful microalgae from coastal waters of the Campania region (Tyrrhenian Sea, Mediterranean Sea). Harmful Algae 5: 321-337. http://dx.doi.org/10.1016/j.hal.2005.09.002