Pérdida de variabilidad genética en una población de cultivo de lenguado senegalés (Solea senegalensis) revelada mediante datos de secuencia de la región control del ADN mitocondrial y de marcadores microsatélite

Autores/as

  • Pablo Sánchez Departament d’Enginyeria Agroalimentària i Biotecnologia, Universitat Politècnica de Catalunya - Centre de Referència de Recerca i Desenvolupament en Aqüicultura de la Generalitat de Catalunya
  • Jordi Viñas Department of Marine Biology, Texas A&M University at Galveston - Laboratori d’Ictiologia Genètica, Departament de Biologia, Universitat de Girona
  • Jaime R. Alvarado Bremer Department of Marine Biology, Texas A&M University at Galveston - Department of Wildlife and Fisheries Sciences, Texas A&M University
  • Pedro Pablo Ambrosio Departament d’Enginyeria Agroalimentària i Biotecnologia, Universitat Politècnica de Catalunya - Centre de Referència de Recerca i Desenvolupament en Aqüicultura de la Generalitat de Catalunya
  • Rosa Flos Departament d’Enginyeria Agroalimentària i Biotecnologia, Universitat Politècnica de Catalunya - Centre de Referència de Recerca i Desenvolupament en Aqüicultura de la Generalitat de Catalunya

DOI:

https://doi.org/10.3989/scimar.03232.07E

Palabras clave:

Solea senegalensis, variabilidad genética, región control mitocondrial, loci microsatélite, gestión de reproductores, peces planos, varianza reproductiva

Resumen


Se compararon los niveles de variabilidad genética de la F1 de una población de cultivo (FAR, n=116) de lenguado senegalés, Solea senegalensis, y de una muestra de su población de origen (ATL, n=26), ambas provenientes del SO de la península ibérica (Atlántico), así como entre esta última y una muestra de individuos salvajes del Mediterráneo occidental (MED, n=18), mediante la caracterización de una secuencia de 412 pares de bases de la Región Control-I del ADN mitocondrial, y de seis loci microsatélite. FAR experimentó una reducción sustancial de variabilidad genética (diversidad haplotípica, h=0.49±0.066; diversidad nucleotídica, π=0.006±0.004; riqueza de alelos privados, pAg=0.28) respecto a su población original ATL (h=0.69±0.114; p=0.009±0.006; pAg=1.21). Los valores de FST entre poblaciones, calculados a partir del análisis de los microsatélites, fueron altamente significativos (p < 0.0001) para FAR y ATL (0.053), y para FAR y MED (0.055). La comparación de las dos muestras salvajes mostró mayores niveles de variabilidad genética en MED que en ATL, pero únicamente en marcadores mitocondriales (h=0.948±0.033; π=0.030±0.016). Sin embargo, los valores de e ΦST y FST presentaron diferencias significativas (p < 0.0001) respectivamente tanto a partir de datos mitocondriales (0.228) como de microsatélites (0.095), aunque la pérdida de variabilidad en FAR podría estar asociada al error de muestreo al establecer el lote de reproductores a partir de peces salvajes, los resultados de la inferencia de parentesco sugieren que la mayor parte de esta pérdida podría estar relacionada con la varianza reproductiva entre los miembros de la generación parental, particularmente entre las hembras.

Descargas

Los datos de descargas todavía no están disponibles.

Citas

Aho T., Rönn J., Piironen J., Björklund M. 2006. Impacts of effective population size on genetic diversity in hatchery reared Brown trout (Salmo trutta L.) populations. Aquaculture 253: 244-248. http://dx.doi.org/10.1016/j.aquaculture.2005.09.013

Alarcón J., Magoulas A., Georgakopoulos T., Zouros E., Álvarez M. 2004. Genetic comparison of wild and cultivated European populations of the gilthead sea bream (Sparus aurata). Aquaculture 230: 65-80. http://dx.doi.org/10.1016/S0044-8486(03)00434-4

Alvarado Bremer J.R., Mejuto J., Baker A.J. 1995. Mitochondrial DNA control region sequences indicate extensive mixing of swordfish (Xiphias gladius L.) populations in the Atlantic Ocean. Can. J. Fish. Aquat. Sci. 52: 1720-1732. http://dx.doi.org/10.1139/f95-764

Alvarado Bremer J.R., Viñas J., Mejuto J., Ely B., Pla C. 2005. Comparative phylogeography of Atlantic bluefin tuna and swordfish: the combined effects of vicariance, secondary contact, introgression, and population expansion on the regional phylogenies of two highly migratory pelagic fishes. Mol. Phylogenet. Evol. 36: 169-187. http://dx.doi.org/10.1016/j.ympev.2004.12.011 PMid :15904864

Anguís V., Cañavate J.P. 2005. Spawning of captive Senegal sole (Solea senegalensis) under a naturally fluctuating temperature regime. Aquaculture 243: 133-145. http://dx.doi.org/10.1016/j.aquaculture.2004.09.026

Blonk R.J.W., Komen J., Kamstra A., Crooijmans R.P.M.A., Van Arendonk J.A.M. 2009. Levels of inbreeding in group mating captive broodstock populations of Common sole, (Solea solea), inferred from parental relatedness and contribution. Aquaculture 289: 26-31. http://dx.doi.org/10.1016/j.aquaculture.2009.01.012

Borsa P., Blanquer A., Berrebi P. 1997. Genetic structure of the flounders Platichthys flesus and P. stellatus at different geographic scales. Mar. Biol. 129: 233-246. http://dx.doi.org/10.1007/s002270050164

Borsa P., Quignard J.P. 2001. Systematics of the Atlantic-Mediterranean soles Pegusa impar, P. lascaris, Solea aegyptiaca, S. senegalensis, and S. solea (Pleuronectiformes: Soleidae). Can. J. Zool. 79: 2297-2302. http://dx.doi.org/10.1139/z01-176

Cañavate, J.P., Fernández-Díaz C. 1999. Influence of co-feeding larvae with live and inert diets on weaning the sole Solea senegalensis onto commercial dry feeds. Aquaculture 174: 255-263. http://dx.doi.org/10.1016/S0044-8486(99)00021-6

Clement M., Posada D., Crandall K.A. 2000. TCS: a computer program to estimate gene genealogies. Mol. Ecol. 9: 1657-1660. http://dx.doi.org/10.1046/j.1365-294x.2000.01020.x PMid :11050560

Cognetti G., Maltagliati F., Saroglia M. 2006. The risk of “genetic pollution” in Mediterranean fish populations related to aquaculture activities. Mar. Pollut. Bull. 52: 1321-1323. http://dx.doi.org/10.1016/j.marpolbul.2006.09.016 PMid :17055003

Coughlan J.P., Imsland A.K., Galvin P.T., Fitzgerald R.D., Naevdal G., Cross, T.F. 1998. Microsatellite DNA variation in wild populations and farmed strains of turbot from Ireland and Norway: a preliminary study. J. Fish. Biol. 52: 916-922. http://dx.doi.org/10.1111/j.1095-8649.1998.tb00592.x

DeWoody J., Avise J. C. 2000. Microsatellite variation in marine, freshwater and anadromous fishes compared with other animals. J. Fish. Biol. 56: 461-473. http://dx.doi.org/10.1111/j.1095-8649.2000.tb00748.x

Dinis M.T., Ribeiro L., Soares F., Sarasquete C. 1999. A review on the cultivation potential of Solea senegalensis in Spain and in Portugal. Aquaculture 176: 27-38. http://dx.doi.org/10.1016/S0044-8486(99)00047-2

Exadactylos A., Geffen A.J., Thorpe J.P. 1998. Population structure of the Dover sole, Solea solea L., in a background of high gene flow. J. Sea. Res. 40: 117-129. http://dx.doi.org/10.1016/S1385-1101(98)00015-X

Exadactylos A., Geffen A.J., Thorpe J.P. 1999. Growth and genetic variation in hatchery-reared larval and juvenile Dover sole, Solea solea (L.). Aquaculture 176: 209-226. http://dx.doi.org/10.1016/S0044-8486(99)00112-X

Excoffier L., Smouse P., Quattro J. 1992. Analysis of molecular variance inferred from the metric distances among DNA haplotypes: Application to human mitochondrial DNA restriction data. Genetics 131: 150-163.

Excoffier L., Lischer H.E.L. 2010, Arlequin suite ver 3.5: A new series of programs to perform population genetics analyses under Linux and Windows. Mol. Ecol. Res. 10: 564-567. http://dx.doi.org/10.1111/j.1755-0998.2010.02847.x PMid :21565059

Falconer D. 1960. Introduction to quantitative genetics. Oliver and Boyd, London.

Fjalestad K. T., Moen T., Gomez-Raya L. 2003. Prospects for genetic technology in salmon breeding programmes. Aquac. Res. 34: 397-406. http://dx.doi.org/10.1046/j.1365-2109.2003.00823.x

Flos R., Reig L., Ambrosio P.P. 2001. Towards the actual transfers of the sole (Solea spp.) to the productive sector. In: Kjorsvik E., Stead S. (eds.), New Species. New Technologies. EAS Special Publication 29: 75-76.

Gavaia P.J., Dinis M.T., Cancela M.L. 2002. Osteological development and abnormalities of the vertebral column and caudal skeleton in larval and juvenile stages of hatchery-reared Senegal sole (Solea senegalensis). Aquaculture 211: 305-323. http://dx.doi.org/10.1016/S0044-8486(02)00167-9

Greig T.W. 2000. Partitioning genetic variation in swordfish (Xiphias gladius L.); Analysis of sample variance and population structure. Ph. D. thesis, Univ. South Carolina.

Guarniero I., Franzellitti S., Ungaro N., Tommasini S., Piccinetti C., Tinti F. 2002. Control region haplotype variation in the central Mediterranean common sole indicates geographical isolation and population structuring in Italian stocks. J. Fish. Biol. 60: 1459-1474. http://dx.doi.org/10.1111/j.1095-8649.2002.tb02440.x

Hall T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl. Acids. Symp. Ser. 41: 95-98.

Imsland A.K., Foss A., Conceição L.E.C., Dinis M.T., Delbare D., Schram E., Kamstra A., Rema P., White P. 2003. A review of the culture potential of Solea solea and S. senegalensis. Rev. Fish. Biol. Fisher. 13: 379-408. http://dx.doi.org/10.1007/s11160-004-1632-6

Kalinowski S.T. 2005. HP-Rare: a computer program for performing rarefaction on measures of allelic diversity. Mol. Ecol. Notes. 5: 187-189. http://dx.doi.org/10.1111/j.1471-8286.2004.00845.x

Kalinowski S.T., Wagner A.P., Taper M.L. 2006. ML-Relate: a computer program for maximum likelihood estimation of relatedness and relationship. Mol. Ecol. Notes. 6: 576-579. http://dx.doi.org/10.1111/j.1471-8286.2006.01256.x

Nei M., Li W.H. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA 76: 5269-5273. http://dx.doi.org/10.1073/pnas.76.10.5269

Nei M., Tajima F. 1981. DNA polymorphism detectable by restriction endonucleases. Genetics. 97: 145-163. PMid :6266912    PMCid:1214380

Nei M. 1987. Molecular evolutionary genetics. Columbia University Press, New York.

Nguyen T.T.T., Ingram B., Sungan S., Gooley G., Sim S.Y., Tinggi D., De Silva S.S. 2006. Mitochondrial DNA diversity of broodstock of two indigenous mahseer species, Tor tambroides and T. douronensis (Cyprinidae) cultured in Sarawak, Malaysia. Aquaculture 253: 259-269. http://dx.doi.org/10.1016/j.aquaculture.2005.09.014

Porta J., Álvarez M. 2004. Development and characterization of microsatellites from Senegal sole (Solea senegalensis). Mol. Ecol. Notes 4: 277-279. http://dx.doi.org/10.1111/j.1471-8286.2004.00640.x

Porta J., Porta J.M., Martínez-Rodríguez G., Álvarez M. 2006. Genetic structure and genetic relatedness of a hatchery stock of Senegal sole (Solea senegalensis) inferred by microsatellites. Aquaculture 251: 46-55. http://dx.doi.org/10.1016/j.aquaculture.2005.05.019

Porta J., Porta J. M., Cañavate P., Martínez-Rodríguez G., Álvarez M. C. 2007. Substantial loss of genetic variation in a single generation of Senegalese sole (Solea senegalensis) culture: implications in the domestication process. J. Fish. Biol. 71: 223-234. http://dx.doi.org/10.1111/j.1095-8649.2007.01576.x

R Development Core Team 2010. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. ISBN 3-900051-07-0, URL http://www.R-project.org/.

Raymond M., Rousset F. 1995. An exact test for population differentiation. Evolution. 49: 1280-1283. http://dx.doi.org/10.2307/2410454

Reig L., Oca J., Flos R. 2000. Trading perspectives of a potential farmed sole (Solea sp) evaluated at the central fish market of Barcelona (Spain). In: Flos R., Creswell L. (eds.), Responsible Aquaculture in the New Millennium. EAS Special Ppublication 28: 596.

Reig L., Ginovart M., Flos R. 2003. Modification of the feeding behaviour of sole (Solea solea) through the addition of a commercial flavour as an alternative to betaine. Aquat. Living. Resour. 16: 370-379. http://dx.doi.org/10.1016/S0990-7440(03)00062-7

Sánchez P., Ambrosio P.P., Reig L., Flos R. 2003. Técnica de biopsia muscular con aguja cortante fina en peces pequeños. In: Consejería de Agricultura y Pesca. Junta de Andalucía. (eds.), Libro de Resúmenes del IX Congreso Nacional de Acuicultura, pp. 360-362.

Sanger F., Nicklen S., Coulson A. R. 1977. DNA sequencing with chain-termination inhibitors. Proc. Natl. Acad. Sci. USA. 74: 5463-5467. http://dx.doi.org/10.1073/pnas.74.12.5463

Sekino M., Hara M., Taniguchi N. 2002. Loss of microsatellite and mitochondrial DNA variation in hatchery strains of Japanese flounder Paralichthys olivaceus. Aquaculture. 213: 101-122. http://dx.doi.org/10.1016/S0044-8486(01)00885-7

Tinti F., Piccinetti C. 2000. Molecular systematics of the Atlanto-Mediterranean Solea species. J. Fish. Biol. 56: 604-614. http://dx.doi.org/10.1111/j.1095-8649.2000.tb00758.x

Tinti F., Piccinetti C., Tommasini S.,Vallisneri M. 2000. Mitochondrial DNA variation, phylogenetic relationships, and evolution of four Mediterranean genera of soles (Soleidae, Pleuronectiformes). Mar. Biotechnol. 2: 274-284.

Van Oosterhout C., Hutchinson W.F., Wills D.P.M., Shipley, P. 2004. MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol. Ecol. Notes. 4(3): 535-538. http://dx.doi.org/10.1111/j.1471-8286.2004.00684.x

Viñas J., Alvarado Bremer J., Pla C. 2004. Inter-oceanic genetic differentiation among albacore (Thunnus alalunga) populations. Mar. Biol. 145(2): 1-8.

Vu A.T. 1997. Molecular characterization of the Cytochrome b-12S rRNA mitochondrial region in the American alligator (Alligator mississippiensis). Honours thesis, Univ. South Carolina.

Wang J. 2004. Sibship reconstruction from genetic data with typing errors. Genetics. 166: 1963-1979. http://dx.doi.org/10.1534/genetics.166.4.1963 PMid :15126412    PMCid:1470831

Ward R.D., Woodwark M., Skibinski D.O.F. 1994. A comparison of genetic diversity levels in marine fresh-water, and anadromous fishes. J. Fish. Biol. 44: 213-232. http://dx.doi.org/10.1111/j.1095-8649.1994.tb01200.x

Zorrilla I., Arijo S., Chabrillón M., Díaz P., Martínez-Manzanares E., Balebona M., Moriñigo M. 2003. Vibrio species isolated from diseased farmed sole, Solea senegalensis (Kaup), and evaluation of the potential virulence role of their extracellular products. J. Fish. Dis. 26: 103-108. http://dx.doi.org/10.1046/j.1365-2761.2003.00437.x PMid :12962218

Descargas

Publicado

2012-06-30

Cómo citar

1.
Sánchez P, Viñas J, Alvarado Bremer JR, Ambrosio PP, Flos R. Pérdida de variabilidad genética en una población de cultivo de lenguado senegalés (Solea senegalensis) revelada mediante datos de secuencia de la región control del ADN mitocondrial y de marcadores microsatélite. Sci. mar. [Internet]. 30 de junio de 2012 [citado 23 de julio de 2024];76(2):225-3. Disponible en: https://scientiamarina.revistas.csic.es/index.php/scientiamarina/article/view/1346

Número

Sección

Artículo Destacado