Tissue effect on RNA:DNA ratios of marine fish larvae

M. Pilar Olivar; Marina V. Diaz; M. Alexandra Chícharo

doi:10.3989/scimar.2009.73s1171

Autores/as

M. Pilar Olivar Institut de Ciències del Mar - CSIC
Marina V. Diaz Instituto Nacional de Investigación y Desarrollo Pesquero
M. Alexandra Chícharo Centre of Marine Sciences, University of Algarve

DOI:

https://doi.org/10.3989/scimar.2009.73s1171

Palabras clave:

ARN, ADN, tejidos, inanición, condición, Sardina pilchardus, Engraulis encrasicolus, Atherina presbyter, Paralichthys orbignyanus

Resumen

En estudios de condición nutricional larvaria basados en la relación ARN:ADN, las cabezas y/o tubos digestivos suelen diseccionarse para posteriores estudios de edad o alimentación. además, durante la captura algunas larvas pierden los ojos. en este trabajo analizamos el efecto de diferentes tejidos (músculo, cabeza, ojos y tubo digestivo) sobre la relación ARN:ADN en Sardina pilchardus, Engraulis encrasicolus, Atherina presbyter y Paralichthys orbignyanus, en diferentes estadios de desarrollo y en diversas regiones. en todas las especies la relación ARN:ADN en la cabeza fue significativamente menor que para el músculo o el tubo digestivo. la comparación de la relación ARN:ADN de la cabeza con o sin ojos no mostró diferencias significativas. al comparar dos secciones de músculo de la cola en Sardina pilchardus y Engraulis encrasicolus se observó mayor ARN:ADN en la parte anterior que en la posterior. es necesaria precaución cuando se comparan ARN:ADN entre estudios que emplean diferentes protocolos de disección. Proponemos factores de conversión para ser aplicados a las relaciones ARN:ADN cuando se analizan diferentes secciones del cuerpo. Se discute cuales son los tejidos más apropiados para determinar la condición nutricional de larvas de peces, basados en las concentraciones de ácidos nucleicos y sus cocientes obtenidos en experimentos de inanición.

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