Bacterial community structure of mangrove sediments in relation to environmental variables accessed by 16S rRNA gene-denaturing gradient gel electrophoresis fingerprinting

Yanying Zhang; Junde Dong; Bin Yang; Juan Ling; Youshao Wang; Si Zhang

doi:10.3989/scimar.2009.73n3487

Autores/as

Yanying Zhang South China Sea Institute of Oceanology, Chinese Academy of Sciences
Junde Dong South China Sea Institute of Oceanology, Chinese Academy of Sciences
Bin Yang South China Sea Institute of Oceanology, Chinese Academy of Sciences
Juan Ling South China Sea Institute of Oceanology, Chinese Academy of Sciences
Youshao Wang South China Sea Institute of Oceanology, Chinese Academy of Sciences
Si Zhang South China Sea Institute of Oceanology, Chinese Academy of Sciences

DOI:

https://doi.org/10.3989/scimar.2009.73n3487

Palabras clave:

comunidad bacteriana, sedimentos de manglares, electroforesis de gradiente de gel desnaturalizante, analisis estadistico multivariante

Resumen

La estructura de la comunidad bacteriana y la relación entre variables ambientales y las comunidades microbianas de la superficie de sedimentos de los ecosistemas tropicales de manglares fueron investigados en Sanya, Hainan Island, China. Los perfiles de las comunidades bacterianas fueron obtenidos utilizando electroforesis de gradiente de gel desnaturalizante (DGE), cultivo-independiente, y los resultados fueron interpretados con un análisis estadístico multivariante. Los resultados sugerían que las comunidades microbianas variaban con los lugares de muestreo y las estaciones. Las muestras recogidas en el mismo lugar de muestreo y al mismo tiempo tenían comunidades microbianas similares, excepto las muestras SH3 y AB5, que tenían diferente calidad de sedimentos. El análisis de correspondencia (CCA) mostró que la concentración de carbono orgánico en sedimentos explicaba una parte importante de la variabilidad en la composición de la comunidad bacteriana. Los análisis filogenéticos fueron usados para identificar los grupos más importantes de filotipos predominantes de bacterias. Se secuenciaron fragmentos 16 rRNA gene-V3 de 17 bandas individuales de DGE y las bacterias correspondientes fueron encontradas por primera vez en sedimentos de manglares en base a resultados de BLAST. Los filotipos de bacterias predominantes se agrupaban con varios grupos taxonómicos que incluyen Proteobacteria, Bacteroidetes, Gemmatimonadetes, Actinobacteria y Firmicutes.

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Biografía del autor/a

Yanying Zhang, South China Sea Institute of Oceanology, Chinese Academy of Sciences

National Experiment Station of Tropical Marine Biology, China Key Laboratory of Marine Bio-resources Sustainable Utilisation (LMB), China Graduate School of Chinese Academy of Science, Beijing, P. R. China

Junde Dong, South China Sea Institute of Oceanology, Chinese Academy of Sciences

National Experiment Station of Tropical Marine Biology, China Key Laboratory of Marine Bio-resources Sustainable Utilisation (LMB), China

Bin Yang, South China Sea Institute of Oceanology, Chinese Academy of Sciences

National Experiment Station of Tropical Marine Biology, China Key Laboratory of Marine Bio-resources Sustainable Utilisation (LMB), China

Juan Ling, South China Sea Institute of Oceanology, Chinese Academy of Sciences

National Experiment Station of Tropical Marine Biology, China. Key Laboratory of Marine Bio-resources Sustainable Utilisation (LMB), China Graduate School of Chinese Academy of Science, Beijing, P. R. China

Si Zhang, South China Sea Institute of Oceanology, Chinese Academy of Sciences

Key Laboratory of Marine Bio-resources Sustainable Utilisation (LMB), China

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