<i>In vitro</i> metabolism of steroid hormones by ovary and hepatopancreas of the crustacean Penaeid shrimp <i>Marsupenaeus japonicus</i>

Teresa Summavielle; Pedro Riberiro Rocha Monteiro; María Armanda Reis-Henriques; Joao Coimbra

doi:10.3989/scimar.2003.67n3299

In vitro metabolism of steroid hormones by ovary and hepatopancreas of the crustacean Penaeid shrimp Marsupenaeus japonicus

Authors

Teresa Summavielle CIMAR - Centre of Marine and Environmental Research, University of Porto (UP)
Pedro Riberiro Rocha Monteiro CIMAR - Centre of Marine and Environmental Research, University of Porto (UP)
María Armanda Reis-Henriques CIMAR - Centre of Marine and Environmental Research, University of Porto (UP)
Joao Coimbra CIMAR - Centre of Marine and Environmental Research, University of Porto (UP)

DOI:

https://doi.org/10.3989/scimar.2003.67n3299

Keywords:

crustacean, decapods, hepatopancreas, hormones, maturation, ovary, reproduction, steroids

Abstract

Radioimmunoassay (RIA) was applied to identify selected metabolites obtained in in vitro incubations of ovarian and hepatopancreatic tissues from female Marsupenaeus japonicus in previtellogenic and vitellogenic maturation stages. Depending on the precursors used, progesterone, 17?-hydroxyprogesterone or testosterone, it was possible to observe the presence of several enzymatic activities through metabolites detected in incubations. The present study provides evidence that the ovary of the shrimp M. japonicus is capable of synthesising 17?-estradiol from progesterone, evidencing the presence of 17?-hydroxylase, C₁₇-C₂₀ lyase, 17?-hydroxysteroid dehydrogenase (17?-HSD) and aromatase. Enzymatic activities of aromatase, 3?-HSD and 17?-hydroxylase were detected in hepatopancreas, but not that of C₁₇-C₂₀ lyase. These results suggest that 17?-estradiol could be the vitellogenesis stimulating ovarian hormone (VSOH), and together with former in vivo and in vitro studies this leads us to propose that in fact there may be a physiological role for steroid hormones in the maturation cycle of crustaceans.

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Published

2003-09-30

How to Cite

Summavielle T, Riberiro Rocha Monteiro P, Reis-Henriques MA, Coimbra J. In vitro metabolism of steroid hormones by ovary and hepatopancreas of the crustacean Penaeid shrimp Marsupenaeus japonicus. Sci. mar. [Internet]. 2003Sep.30 [cited 2024Apr.19];67(3):299-306. Available from: https://scientiamarina.revistas.csic.es/index.php/scientiamarina/article/view/479

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Issue

Vol. 67 No. 3 (2003)

Section

Articles

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