Flow cytometry as a tool for the study of phytoplankton
DOI:
https://doi.org/10.3989/scimar.2000.64n2135Keywords:
flow cytometry, phytoplankton, marine, fresh water, monitoring, counting, identification, fluorescence, particle analysisAbstract
An overview is presented on flow cytometry as a tool for counting, analysis and identification of phytoplankton species and groups. The paper covers basics on the analysis technique and instrumentation such as the measuring principle, the type of instrument, limitations and pitfalls with phytoplankton samples and sample handling and preprocessing. Possibilities of the measured entities are discussed, roughly divided in light scatter and related parameters, the endogenous fluorescence and exogenous fluorescence, followed by a discussion on the actual applications such as phytoplankton abundance analysis, ecology and physiology research and monitoring of particle size and biomass. In addition to a limited literature review, we tried to assess how flow cytometry is used in routine laboratory practice and monitoring operations. Therefore, a questionnaire was sent out via email to 47 scientists at 43 institutes known to us as involved in flow cytometric analysis of phytoplankton. In total, 19 scientists responded. Specific survey results are included in italic print whereas some more general answers were integrated in the overview.
Downloads
Download data is not yet available.
Downloads
Published
2000-06-30
How to Cite
1.
Dubelaar GB, Jonker RR. Flow cytometry as a tool for the study of phytoplankton. scimar [Internet]. 2000Jun.30 [cited 2023Nov.28];64(2):135-56. Available from: https://scientiamarina.revistas.csic.es/index.php/scientiamarina/article/view/751
Issue
Section
Articles
License
Copyright (c) 2000 Consejo Superior de Investigaciones Científicas (CSIC)

This work is licensed under a Creative Commons Attribution 4.0 International License.
© CSIC. Manuscripts published in both the printed and online versions of this Journal are the property of Consejo Superior de Investigaciones Científicas, and quoting this source is a requirement for any partial or full reproduction.All contents of this electronic edition, except where otherwise noted, are distributed under a “Creative Commons Attribution 4.0 International” (CC BY 4.0) License. You may read here the basic information and the legal text of the license. The indication of the CC BY 4.0 License must be expressly stated in this way when necessary.
Self-archiving in repositories, personal webpages or similar, of any version other than the published by the Editor, is not allowed.