PCR-SSCP of the 16S rRNA gene, a simple methodology for species identification of fish eggs and larvae

Authors

  • Eva Garcia-Vazquez Departamento Biología Funcional, Universidad de Oviedo.
  • Paula Alvarez Fundación AZTI. Departamento de Recursos Pesqueros. Pasaia (Guipúzcoa)
  • Placida Lopes Instituto de Investigaçao das Pescas e do Mar.
  • Nikoletta Karaiskou Department of Genetics, Development and Molecular Biology, School of Biology, Aristotle University of Thessaloniki
  • Juliana Perez Departamento Biología Funcional, Universidad de Oviedo.
  • Ana Teia Instituto de Investigaçao das Pescas e do Mar.
  • José L. Martinez Departamento Biología Funcional, Universidad de Oviedo.
  • Laurentina Gomes Instituto de Investigaçao das Pescas e do Mar.
  • Costas Triantaphyllidis Department of Genetics, Development and Molecular Biology, School of Biology, Aristotle University of Thessaloniki

DOI:

https://doi.org/10.3989/scimar.2006.70s213

Keywords:

fish species, genetic markers, ichthyoplankton, mitochondrial genes, PCR-SSCP

Abstract


Patterns of the 16S rRNA gene obtained in 8 and 12% acrylamide gels by the SSCP (Single Strand Conformation Polymorphism) method were different for various marine fish species (Macrorhamphosus scolopax, Scomber scombrus, Lepidorhombus boscii, L. whiffiagonis, Trachurus trachurus, T. mediterraneus, Molva molva, Merluccius merluccius). SSCP patterns of this gene were employed to successfully identify formaldehyde-fixed eggs of different species (Merluccius merluccius, Scomber scombrus, Macrorhamphosus scolopax and L. whiffiagonis) in plankton samples. The advantages of SSCPs in comparison with current genetic methods of egg identification are based on their technical simplicity and low price. The application of the PCR-SSCP methodology is proposed for routine genetic analyses in plankton surveys.

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Published

2006-10-30

How to Cite

1.
Garcia-Vazquez E, Alvarez P, Lopes P, Karaiskou N, Perez J, Teia A, Martinez JL, Gomes L, Triantaphyllidis C. PCR-SSCP of the 16S rRNA gene, a simple methodology for species identification of fish eggs and larvae. scimar [Internet]. 2006Oct.30 [cited 2021Sep.17];70(S2):13-21. Available from: https://scientiamarina.revistas.csic.es/index.php/scientiamarina/article/view/164

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Articles