DNA extraction from formalin-fixed tissue: new light from the deep sea


  • Ferran Palero Evolutionary Genetics, Institute of Science and Technology Austria (ISTA)
  • Sally Hall National Oceanography Centre, Southampton, School of Ocean and Earth Science, University of Southampton
  • Paul F. Clark Department of Zoology, Natural History Museum
  • David Johnston Department of Zoology, Natural History Museum
  • Jackie Mackenzie-Dodds Department of Zoology, Natural History Museum
  • Sven Thatje National Oceanography Centre, Southampton, School of Ocean and Earth Science, University of Southampton




tetramethylsilane, ethidium bromide, formalin, Carcinus, Lithodidae


DNA samples were extracted from ethanol and formalin-fixed decapod crustacean tissue using a new method based on Tetramethylsilane (TMS)-Chelex. It is shown that neither an indigestible matrix of cross-linked protein nor soluble PCR inhibitors impede PCR success when dealing with formalin-fixed material. Instead, amplification success from formalin-fixed tissue appears to depend on the presence of unmodified DNA in the extracted sample. A staining method that facilitates the targeting of samples with a high content of unmodified DNA is provided.


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How to Cite

Palero F, Hall S, Clark PF, Johnston D, Mackenzie-Dodds J, Thatje S. DNA extraction from formalin-fixed tissue: new light from the deep sea. Sci. mar. [Internet]. 2010Sep.30 [cited 2024Feb.21];74(3):465-70. Available from: https://scientiamarina.revistas.csic.es/index.php/scientiamarina/article/view/1175




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