Barcoding coffee grounds - Exploring pteropod gastropod biodiversity with dregs in collection jars

Christina Franziska Laibl; Juan Lucas Cervera Currado; Jérôme Morinière; Michael Schrödl

doi:10.3989/scimar.05275.061

Autores/as

Christina Franziska Laibl SNSB-Bavarian State Collection of Zoology - Ludwig-Maximilians-Universität München, Faculty of Biology https://orcid.org/0000-0002-0306-3467
Juan Lucas Cervera Currado Departamento de Biología, Facultad de Ciencias del Mar y Ambientales, Campus de Excelencia Internacional del Mar (CEIMAR), Universidad de Cádiz - Instituto Universitario de Investigación Marina (INMAR), Campus de Excelencia Internacional del Mar (CEIMAR), Universidad de Cádiz https://orcid.org/0000-0002-8337-2867
Jérôme Morinière AIM - Advanced Identification Methods GmbH https://orcid.org/0000-0001-9167-6409
Michael Schrödl SNSB-Bavarian State Collection of Zoology - Ludwig-Maximilians-Universität München, Faculty of Biology - GeoBioCentrer LMU, Centre of Geobiology and Biodiversity Research at the Ludwig-Maximilians-University Munich https://orcid.org/0000-0002-7629-1911

DOI:

https://doi.org/10.3989/scimar.05275.061

Palabras clave:

Mollusca, Gastropoda, plancton, ADN ambiental, circumglobal, diversidad de pterópodos, expedición Malaspina

Resumen

A pesar de su presencia cosmopolita y las actividades de muestreo masivo de plancton durante las expediciones, la diversidad genética dentro de los Pteropoda Cuvier, 1804 está todavía inexplorada en gran medida. En este estudio se presenta una aproximación desde el barcoding ambiental aplicada a muestras generales de zooplancton recogidas durante la expedición circumglobal “Malaspina 2010”, con el fin de evaluar la diversidad de pterópodos. Se introduce una técnica que evita procedimientos destructivos de tal modo que el material permanece intacto para futuras investigaciones morfológicas. Extrajimos ADN de los posos (material orgánico como moco o partes del cuerpo) de 27 recipientes de muestras para el barcoding (promedio de 100- 260 bp de COI). Se pudieron identificar 7128 “OTUs” correspondientes a la composición de las especies contenidas en las muestras examinadas. Entre ellas se encontraron tres especies de pterópodos tecosomados, Creseis acicula, Creseis virgula y Cavolinia inflexa, cuya taxonomía y distribución geográfica son discutidas. Gimnosomados no identificados procedentes de regiones más templadas de aguas oceánicas del sur del Océnao Indico también estaban presentes. Para facilitar la identificación de especies, es beneficioso crear una base de datos ampliada de códigos de barras COI de pterópodos. Además, la recopilación de datos de barcoding ambiental a una escala mundial amplia ayudará a comprender mejor la abundancia y distribución de especies de pterópodos en los océanos del mundo y de otros posibles organismos planctónicos.

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Citas

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