Morphology and rDNA phylogeny of a Mediterranean <em>Coolia monotis</em> (Dinophyceae) strain from Greece

Nicolas P. Dolapsakis; Michael W. Kilpatrick; Athena Economou-Amilli; Triantafyllos Tafas

doi:10.3989/scimar.2006.70n167

Autores/as

Nicolas P. Dolapsakis Department of Ecology and Systematics, Faculty of Biology, University of Athens
Michael W. Kilpatrick Ikonisys Inc.
Athena Economou-Amilli Department of Ecology and Systematics, Faculty of Biology, University of Athens
Triantafyllos Tafas Department of Ecology and Systematics, Faculty of Biology, University of Athens

DOI:

https://doi.org/10.3989/scimar.2006.70n167

Palabras clave:

Coolia monotis, Grecia, microscopía, rDNA, taxonomía, filogenia

Resumen

Las secuencias de los genes del RNA de las subunidades ribosomales grandes y pequeñas (LSU y SSU, respectivamente) y la filogenia del dinoflagelado Coolia monotis Meunier han sido poco investigadas, y no hay información disponible sobre los genes LSU y SSU de subespecies mediterráneas. Una subespecie aislada del golfo de Thermaikos en el norte de Grecia fue identificada como C. monotis –una nueva aportación a la flora algal griega– por medio de la morfología de la teca observada a través de microscopía óptica, de epifluorescia y electrónica. Las secuencias correspondientes a la subunidad pequeña y a la parte (D1/D2) de la subunidad grande fueron analizadas y comparadas a las de otras subespecies de C. monotis y otras especies de dinoflagelados de diversas regiones. La arquitectura de la teca mostró que la subespecie griega de C. monotis era fenotípicamente similar, pero no idéntica, a otras subespecies registradas en la literatura. Se encontró que la secuencia parcial de la LSU (700 pares de bases o bp) difería de la de C. monotis de Nueva Zelanda en las posiciones de 113 bp (16%), mientras que la SSU (1757 bp) se diferenciaba en 15 bp (0.85%) de la subespecie de Noruega. La construcción del árbol filogenetico demostró que la subespecie griega se situaba dentro de la rama de Coolia y presentaba una relación cercana con las familias Ostreopsidaceae y Goniodomaceae del orden Gonyaulacales. Resultados preliminares sugieren la existencia de diversos genotipos de la subespecie de C. monotis con una importante variabilidad genética intraespecífica y una mínima diferenciación morfológica (fenotipos similares). Se comentan diversas implicaciones ecológicas y evolutivas de estos resultados.

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Biografía del autor/a

Triantafyllos Tafas, Department of Ecology and Systematics, Faculty of Biology, University of Athens

Ikonisys Inc., 5 Science Park, New Haven 06511, Connecticut, USA

Citas

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