Glycohistochemical study of the toadfish <em>Halobatrachus didactylus</em> (Scheider, 1801) stomach

Salvatore Desantis; Franca Acone; Sara Zizza; Michele Deflorio; Josè Luis Palazón Fernández; Carmen Sarasquete; Gregorio De Metrio

doi:10.3989/scimar.2009.73n3515

Autores/as

Salvatore Desantis Faculty of Veterinary Medicine, University of Bari
Franca Acone Faculty of Veterinary Medicine, University of Sassari
Sara Zizza Faculty of Veterinary Medicine, University of Bari
Michele Deflorio Faculty of Veterinary Medicine, University of Bari
Josè Luis Palazón Fernández Universidad de Oriente, Escuela de Ciencias Aplicadas del Mar
Carmen Sarasquete Instituto de Ciencias Marinas de Andalucía (CSIC)
Gregorio De Metrio Faculty of Veterinary Medicine, University of Bari

DOI:

https://doi.org/10.3989/scimar.2009.73n3515

Palabras clave:

glicoconjugados, histoquímica de lectinas, estómago, pez sapo

Resumen

Se ha estudiado la expresion de residuos glucidicos de glucoconjugados en la mucosa gastrica del pez sapo, Halobatrachus didactylus usando histoquimica convencional y de lectinas. La histoquimica clasica revelo la presencia de glicoconjugados neutros predominando sobre los acidos en la zona apical de la superficie de las celulas epiteliales y de la criptas gastricas. Las celulas del cuello contienen algunas glicoproteinas neutras, mientras las glandulas gastricas han sido negativas al PAS y al Azul Alcian (AB). La histoquimica de lectinas mostro diferente expresion de oligosacaridos a lo largo de las celulas columnares. El citoplasma sub-nuclear reacciono con las lectinas RCA, SBA, HPA, GSA, I-B4, GSA II, UEA I, LTA, Con A, KOH-sialidasa-WGA. La expresion de RCA120, DBA, SBA, HPA, KOH-sialidasa-WGA, GSA I-B₄, GSA II, UEA I, LTA, MAL II y SNA se localizo en la zona del Golgi, donde se observo un incremento de la reactividad de la lectina DBA despues del tratamiento con KOH. Los granulos de la zona apical reaccionaron con PNA, UEA I, LTA, mostrando un incremento de la reactividad hacia la lectina PNA despues del tratamiento con KOH. El borde de las celulas del lumen reacciono con PNA, HPA, ConA, KOH-sialidasa-WGA, UEA I, LTA, MAL II, SNA, KOH-sialidasa-PNA. Los sitios de union de la expresion de las lectinas-glucoconjugados fue menor en las celulas del cuello de las criptas gastricas que en las celulas columnares epiteliales. Las celulas del cuello reaccionaron con la UEA I y LTA, y las glandulas gastricas con PNA, DBA, SBA, HPA, Con A, GSA I-B₄, KOH-sialidasa-WGA y KOH-sialidasa-DBA. Los resultados sugieren que el estomago del pez sapo, Halobatrachus didactylus, se caracteriza por un patron especie-especifico de expresion de glicoconjugados.

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