Antimicrobial activity of Iberian macroalgae


  • Noemí Salvador Laboratori de Botànica, Facultat de Farmàcia, Universitat de Barcelona, Barcelona
  • Amelia Gómez Garreta Laboratori de Botànica, Facultat de Farmàcia, Universitat de Barcelona, Barcelona
  • Luca Lavelli Laboratori de Botànica, Facultat de Farmàcia, Universitat de Barcelona, Barcelona
  • María Antonia Ribera Laboratori de Botànica, Facultat de Farmàcia, Universitat de Barcelona, Barcelona



antimicrobial activity, marine macroalgae, Bonnemaisoniales, agar diffusion technique, crude extracts, Iberian Peninsula


The antibacterial and antifungal activity of 82 marine macroalgae (18 Chlorophyceae, 25 Phaeophyceae and 39 Rhodophyceae) was studied to evaluate their potential for being used as natural preservatives in the cosmetic industry. The bioactivity was analysed from crude extracts of fresh and lyophilised samples against three Gram-positive bacteria, two Gram-negative bacteria and one yeast using the agar diffusion technique. The samples were collected seasonally from Mediterranean and Atlantic coasts of the Iberian Peninsula. Of the macroalgae analysed, 67% were active against at least one of the six test microorganisms. The highest percentage of active taxa was found in Phaeophyceae (84%), followed by Rhodophyceae (67%) and Chlorophyceae (44%). Nevertheless, red algae had both the highest values and the broadest spectrum of bioactivity. In particular, Bonnemaisonia asparagoides, Bonnemaisonia hamifera, Asparagopsis armata and Falkenbergia rufolanosa (Bonnemaisoniales) were the most active taxa. Bacillus cereus was the most sensitive test microorganism and Pseudomonas aeruginosa was the most resistant. The highest percentages of active taxa from Phaeophyceae and Rhodophyceae were found in autumn, whereas they were found in summer for Chlorophyceae.


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Algues et Mer SARL. – 2002. Method of obtaining an antibacterial and/or antifungal extract from the algae, Bonnemaisoniaceae. Inventor: J-Y. Moigne. Off. gaz. U.S. Pat. Trademark Off. Pat., invention patent, US 6346252B1.

Álvarez Benito, Ma. V. – 1990. Manual de técnicas en microbiología clínica. Asociación Española de Farmacéuticos Analistas, San Sebastian.

Arun Kumar, K. and R. Rengasamy. – 2000. Evaluation of antibacterial potential of seaweeds occurring along the coast of Tamil Nadu, India against the plant pathogenic bacterium Xanthomonas oryzae pv. oryzae (Ishiyama) Dye. Bot. Mar., 43: 409-415. doi:10.1515/BOT.2000.042

Baker, J.T. – 1984. Seaweeds in pharmaceutical studies and applications. Hydrobiologia, 116/117: 29-40. doi:10.1007/BF00027635

Ballantine, D.L., W.H. Gerwick, S.M. Velez, E. Alexander and P. Guevara. – 1987. Antibiotic activity of lipid-soluble extracts from Caribbean marine algae. Hydrobiologia, 151/152: 463-469. doi:10.1007/BF00046168

Ballesteros, E., D. Martín and M.J. Uriz. – 1992. Biological activity of extracts from some Mediterranean macrophytes. Bot. Mar., 35: 481-485.

Bhosale, S.H., V.L. Nagle and T.G. Jagtap. – 2002. Antifouling potential of some marine organisms from India species of Bacillus and Pseudomonas. Mar. Biotechnol., 4: 111-118. doi:10.1007/s10126-001-0087-1 PMid:14961269

Burkholder, P.R., L.M. Burkholder and L.R. Almodóvar. – 1960. Antibiotic activity of some marine algae of Puerto Rico. Bot. Mar., 2: 149-156.

Cabañes, F.J., L. Abarca, M.A. Calvo and J.A. Seoane. – 1984. Determinación de la capacidad antifúngica de algas mediterráneas. 2ª Reunión conjunta de micología. Resúmenes de ponencias y comunicaciones, p.171.

Caccamese, S., R. Azzolina, G. Furnari, M. Cormaci and S. Grasso. – 1980. Antimicrobial and antiviral activities of extracts from Mediterranean algae. Bot. Mar., 23: 285-288.

Caccamese, S., R. Azzolina, G. Furnari, M. Cormaci and S. Grasso. – 1981. Antimicrobial and antiviral activities of some marine algae from eastern Sicily. Bot. Mar., 24: 365-367.

Caccamese, S., R.M. Toscano, G. Furnari and M. Cormaci. – 1985. Antimicrobial activities of red and brown algae from southern Italy coast. Bot. Mar., 28: 505-507.

Campos-Takaki, G.M., M.B.S. Diu, M.L. Koening and E.C. Pereira. – 1988. Screening of marine algae from Brazilian northeastern coast for antimicrobial activity. Bot. Mar., 31: 375-377.

Centeno, P.O.R. and D.L. Ballantine. – 1999. Effects of culture conditions on production of antibiotically active metabolites by the marine alga Spyridia filamentosa. J. Appl. Phycol., 10: 453-460. doi:10.1023/A:1008038418777

Chesters, C.G. and J.A. Stott. – 1956. Production of antibiotic substances by seaweeds. Proc. Int. Seaweed Symp., 2: 49-54.

Crasta, P.J., N.S. Raviraja and K.R. Sridhar. – 1997. Antimicrobial activity of some marine algae of southwest coast of India. Indian J. Mar. Sci., 26: 201-205.

Della Pietà, F., L. Panizzi, P.L. Cioni, F. Cinelli and I. Morelli. – 1996. Attività antimicrobica di estratti di Caulerpa taxifolia su batteri e micetti terrestri. In: M.A. Ribera, E. Ballesteros, C.F. Boudouresque, A. Gómez and V. Gravez (eds.). Second International Workshop on Caulerpa taxifolia, pp. 261-264. Publ. Univ. Barcelona, Barcelona.

Espeche, M.E., E.R. Fraile and A.M.S. Mayer. – 1984. Screening of Argentine marine algae for antimicrobial activity. Hydrobiologia, 116/117: 525-528. doi:10.1007/BF00027738

Febles, C.I., A. Arias, A. Hardisson, A. Sierra López and M.C. Gil- Rodríguez. – 1995. Antimicrobial activity of extracts from some Canary species of Phaeophyta and Chlorophyta. Phytotherapy Res., 9: 385-387. doi:10.1002/ptr.2650090517

Fenical, W. and V.J. Paul. – 1984. Antibiotic and cytotoxic terpenoids from tropical green algae of the family Udoteaceae. Hidrobiologia, 116/117: 137-140.

Glombitza, K.W. – 1970. Antimicrobial constituents in algae. Quantitative determination of acrylic acid in sea-algae. Planta Med., 18: 210-221.

Harder, R. – 1917. Ernährungsphysiologische Untersuchungen an Cyanophyceen, hauptsächlich am endophytischen Nostoc punctiforme. Z. Bot., 9: 145.

Henríquez, P., R. Zemelman, M.A. Moncada and I.L. Benoit. – 1977. Propiedades antibióticas de algas marinas. Bol. Soc. Biol. Concepción, 11: 119-122.

Hodgson, L.M. – 1984. Antimicrobial and antineoplastic activity in some south Florida seaweeds. Bot. Mar., 27: 387-390.

Horikawa, M., T. Noro and Y. Kamel. – 1999. In vitro anti-methicillin- resistant Staphylococcus aureus. Activity found in extracts of marine algae indigenous to the coastline of Japan. J. Antibiot., 52: 186-189.

Hornsey, I.S. and D. Hide. – 1974. The production of antimicrobial compounds by British marine algae. I. Antibiotic-producing marine algae. Br. Phycol. J., 9: 353-361. doi:10.1080/00071617400650421

Hornsey, I.S. and D. Hide. – 1985. The production of antimicrobial compounds by British marine algae. IV. Variation of antimicrobial activity with algal generation. Br. Phycol. J., 20: 21-25. doi:10.1080/00071618500650041

Khaleafa, A.F., M.A.M. Kharboush, A. Metwalli, A.F. Mohsen and A. Serwi. – 1975. Antibiotic (fungicidal) action from extracts of some seaweeds. Bot. Mar., 18: 163-165.

König, G.M., A.D. Wright, O. Stiche, C.K. Angerhofer and J.M. Pezzuto. – 1994. Biological activities of selected marine natural products. Planta Med., 60: 532-537. doi:10.1055/s-2006-959565 PMid:7809206

Ma, J.W. and W.C. Tang. – 1984. Screening for antimicrobial activities in marine algae from the Qingdao coast, China. Hydrobiologia, 116/117: 517-520. doi:10.1007/BF00027736

Martí, R., M.J. Uriz and X. Turon. – 2004. Seasonal and spatial variation of species toxicity in Mediterranean seaweed communities: correlation to biotic and abiotic factors. Mar. Ecol. Prog. Ser., 282: 73-85. doi:10.3354/meps282073

Melo, V.M.M., D.A. Medeiros, F.J.B. Rios, L.I.M. Castelar and A. de F.F.U. Carvalho. –1997. Antifungal properties of proteins (agglutinins) from the red alga Hypnea musciformis (Wulfen) Lamouroux. Bot. Mar., 40: 281-284.

Moreau, J., D. Pesando, and B. Caram. – 1984. Antifungal and antibacterial screening of Dictyotales from the French Mediterranean coast. Hydrobiologia, 116/117: 521-524. doi:10.1007/BF00027737

Muñoz, A. – 1992. Drogas del mar. Sustancias biomédicas de algas marinas. Servicio de Publicaciones e Intercambio Científico, Universidad de Santiago de Compostela, Santiago de Compostela.

Naqvi, S.W.A., S.Y. Kamat, L. Fernandes, C.V.G. Reddy. – 1980. Screening of some marine plants from the Indian coast for biological activity. Bot. Mar., 24: 51-55.

Navarro, J.N., A. Rodríguez, N. Vásquez, A. Sánchez and M.A. Rivera. – 1990. Efectos antibióticos de las algas marinas de Puerto Rico. Rev. Fac. Ocean. Pesq. Cs. Alimentarias, 2: 165-173.

Padmakumar, K. and K. Ayyakkannu. – 1997. Seasonal variation of antibacterial and antifungal activities of the extracts of marine algae from southern coasts of India. Bot. Mar., 40: 507-515.

Padmini Sreenivasa Rao, P., P. Sreenivasa Rao and S.M. Karmarkar. – 1986. Antibacterial substances from brown algae. II. Efficiency of solvents in the evaluation of antibacterial substances from Sargassum johnstonii Setchell et Gardner. Bot. Mar., 29: 503-507.

Pérez, G.R.M., A.J.G. Avila, G.S. Pérez, C.A. Martínez and C.G. Martínez. – 1990. Antimicrobial activity of some American algae. J. Ethnopharmacol., 29: 111-116. doi:10.1016/0378-8741(90)90104-2 PMid:2345456

Pesando, D. – 1990. Antibacterial and antifungal activities of marine algae. In: I. Akatsuka (ed.), Introduction to Applied Phycology, pp. 3-26. SPB Academic Publishing, The Hague.

Pesando, D. and B. Caram. – 1984. Screening of marine algae from the French Mediterranean coast for antibacterial and antifungal activity. Bot. Mar., 27: 381-386.

Pratt, R., R.H. Mautner, G.M. Gardner, Y. Sha and F. Dufrenoy. – 1951. Report on the antibiotic activity of seaweed extracts. J. Amer. Pharm. Assoc. Sci. Edn., 40: 575-579.

Reichelt, J.L. and M.A. Borowitzka. – 1984. Antimicrobial activity from marine algae: results of a large-scale screening programme. Hydrobiologia, 116/117: 158-168. doi:10.1007/BF00027657

Rosell, K.G. and L.M. Srivastava. – 1987. Fatty acids as antimicrobial substances in brown algae. Hydrobiologia, 151/152: 471-475. doi:10.1007/BF00046169

Salvador Soler, N., A. Gómez Garreta, and M.A. Ribera Siguan. – 2006. Mapas de distribución de algas marinas de la Península Ibérica y las islas Baleares. Bonnemaisonia (Bonnemaisoniaceae, Rhodophyta). Bot. Complut., 30: 159-164.

Seguin, M.C., A. Franco, E. Feno, J.Y. Moigne and F. Bresdin. – 1995. Extraction de composés organiques de silicium biologiquement actifs d’origine algale. Brevet nº FR 2 732 022.

Serarols, M.D., M.C. Hernández and J.A. Seoane. – 1982. Sobre la actividad antibiótica de ciertas especies de algas del Mediterráneo. Collect. Bot. (Barcelona), 13: 919-927.

Sreenivasa Rao, P. and K.S. Parekh. – 1981. Antibacterial activity of Indian seaweed extracts. Bot. Mar., 24: 577-582.

Usmanghani, K., M. Shameel, M. Sualeh, K.H. Khan and Z.A. Mahmood. – 1984. Antibacterial and antifungal activities of marine algae from Karachi seashore of Pakistan. Fitoterapia, 55: 73-77.

Welch, A.M. – 1962. Preliminary survey fungistatic properties of marine algae. J. Bacteriol., 83: 97-99.




How to Cite

Salvador N, Gómez Garreta A, Lavelli L, Ribera MA. Antimicrobial activity of Iberian macroalgae. scimar [Internet]. 2007Mar.30 [cited 2022Dec.2];71(1):101-14. Available from: