Glycohistochemical study of the toadfish <em>Halobatrachus didactylus</em> (Scheider, 1801) stomach

Salvatore Desantis; Franca Acone; Sara Zizza; Michele Deflorio; Josè Luis Palazón Fernández; Carmen Sarasquete; Gregorio De Metrio

doi:10.3989/scimar.2009.73n3515

Authors

Salvatore Desantis Faculty of Veterinary Medicine, University of Bari
Franca Acone Faculty of Veterinary Medicine, University of Sassari
Sara Zizza Faculty of Veterinary Medicine, University of Bari
Michele Deflorio Faculty of Veterinary Medicine, University of Bari
Josè Luis Palazón Fernández Universidad de Oriente, Escuela de Ciencias Aplicadas del Mar
Carmen Sarasquete Instituto de Ciencias Marinas de Andalucía (CSIC)
Gregorio De Metrio Faculty of Veterinary Medicine, University of Bari

DOI:

https://doi.org/10.3989/scimar.2009.73n3515

Keywords:

glycoconjugates, lectin histochemistry, stomach, toadfish

Abstract

Toadfish Halobatrachus didactylus gastric mucosa was studied using conventional and lectin histochemistry. Conventional histochemistry revealed neutral glycoconjugates predominating over acidic ones in the apical zone of both surface epithelial cells and pit cells. The neck cells contained a few neutral glycoconjugates, whereas gastric glands were negative to PAS and AB staining. Lectin histochemistry showed different oligosaccharide expression along the columnar cells. The sub-nuclear cytoplasm was stained with RCA₁₂₀, SBA, HPA, GSA I-B₄, GSA II, UEA I, LTA, Con A, KOH-sialidase-WGA. The Golgi zone reacted with RCA₁₂₀, DBA, SBA, HPA, KOH-sialidase-WGA, GSA I-B₄, GSA II, UEA I, LTA, MAL II, SNA, and showed an increase in DBA staining after KOH-sialidase treatment. The granules of the apical zone stained with PNA, UEA I, LTA and showed increased PNA reactivity after KOH-sialidase treatment. The luminal cell coat reacted with PNA, HPA, Con A, KOH-sialidase-WGA, UEA I, LTA, MAL II, SNA and KOH-sialidase-PNA. Pit cells showed a minor expression of lectin-binding sites with respect to columnar cells. Neck cells linked UEA I and LTA and gastric glands reacted with PNA, DBA, SBA, HPA, Con A, GSA I-B₄, KOH-sialidase-WGA and KOH-sialidase-DBA. The results suggest that the stomach of the toadfish H. didactylus is characterised by a species-specific glycoconjugate pattern.

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