Glycohistochemical study of the toadfish Halobatrachus didactylus (Scheider, 1801) stomach


  • Salvatore Desantis Faculty of Veterinary Medicine, University of Bari
  • Franca Acone Faculty of Veterinary Medicine, University of Sassari
  • Sara Zizza Faculty of Veterinary Medicine, University of Bari
  • Michele Deflorio Faculty of Veterinary Medicine, University of Bari
  • Josè Luis Palazón Fernández Universidad de Oriente, Escuela de Ciencias Aplicadas del Mar
  • Carmen Sarasquete Instituto de Ciencias Marinas de Andalucía (CSIC)
  • Gregorio De Metrio Faculty of Veterinary Medicine, University of Bari



glycoconjugates, lectin histochemistry, stomach, toadfish


Toadfish Halobatrachus didactylus gastric mucosa was studied using conventional and lectin histochemistry. Conventional histochemistry revealed neutral glycoconjugates predominating over acidic ones in the apical zone of both surface epithelial cells and pit cells. The neck cells contained a few neutral glycoconjugates, whereas gastric glands were negative to PAS and AB staining. Lectin histochemistry showed different oligosaccharide expression along the columnar cells. The sub-nuclear cytoplasm was stained with RCA120, SBA, HPA, GSA I-B4, GSA II, UEA I, LTA, Con A, KOH-sialidase-WGA. The Golgi zone reacted with RCA120, DBA, SBA, HPA, KOH-sialidase-WGA, GSA I-B4, GSA II, UEA I, LTA, MAL II, SNA, and showed an increase in DBA staining after KOH-sialidase treatment. The granules of the apical zone stained with PNA, UEA I, LTA and showed increased PNA reactivity after KOH-sialidase treatment. The luminal cell coat reacted with PNA, HPA, Con A, KOH-sialidase-WGA, UEA I, LTA, MAL II, SNA and KOH-sialidase-PNA. Pit cells showed a minor expression of lectin-binding sites with respect to columnar cells. Neck cells linked UEA I and LTA and gastric glands reacted with PNA, DBA, SBA, HPA, Con A, GSA I-B4, KOH-sialidase-WGA and KOH-sialidase-DBA. The results suggest that the stomach of the toadfish H. didactylus is characterised by a species-specific glycoconjugate pattern.


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Arellano, J.M., V. Storch and C. Sarasquete. – 2001. Histological and histochemical observations in the stomach of the Senegal sole, Solea senegalensis. Histol. Histopathol., 16: 511-521.

Arias, A. – 1976. Contribucion al conocimiento de la fauna bentonita de la Bahia de Cadiz. Invest. Pesq., 40: 355-386.

Arias, A.M.A. and P. Drake. – 1990. Estados juveniles de la ictiofauna en los canos de las salinas de la Bahia de Cadiz. Consejo Superior de Investigaciones Cientificas, Cadiz, Spain.

Cardenas, S. – 1977. Regimen alimenticio del sapo, Halobatrachus didactylus (Schneider, 1801) (Pisces: batrachoididae), en la bahia de Cadiz (Espana sudoccidental). Vie Milieu, 28: 111-129.

Carasson, M., A. Grau, L.R. Dopazo and S.A. Crespo. – 2006. Histological, histochemical and ultrastructural study of the digestive tract of Dentex dentex (Pisces, Sparidae). Histol. Histopathol., 21: 579-593.

Danguy, A., F. Afik, B. Pajak and H.J. Gabius. – 1994. Contribution of carbohydrate histochemistry to glycobiology. Histol. Histopathol., 9: 155-171.

Debray, H., D. Decout, G. Strecker, G. Spik and J. Montreuil. – 1981. Specificity of twelve lectins towards oligosaccharides and glycopeptides related to N-glycosylproteins. Eur. J. Biochem., 117: 41-55.

Desantis, S., F. Cirillo, M. Deflorio, P. Megalofonou, J.L. Palazon, C. Sarasquete and G. De Metrio. – 2007. Histochemical study of glycoconjugates in the toadfish Halobatrachus didactylus oesophagus epithelium. Histol. Histopathol., 22: 23-35.

Diaz, A.O., A.M. Garcia and A.L. Goldemberg. – 2008. Glycoconjugates in the mucosa of the digestive tract of Cynoscion guatucupa: a histochemical study. Acta Histochem., 110: 76-85. doi:10.1016/j.acthis.2007.08.002

Domeneghini, C., R. Pannelli Straini and A. Veggetti. – 1998. Gut glycoconjugates in Sparus aurata L. (Pisces, Teleostei). A comparative histochemical study in larval and adult ages. Histol. Histopathol., 13: 359-372.

Domeneghini, C., S. Arrighi, G. Radaelli, G. Bosi and A. Veggetti. – 2005. Histochemical analysis of glycoconjugate secretion in the alimentary canal of Anguilla anguilla L. Acta Histochem., 106: 477-487. doi:10.1016/j.acthis.2004.07.007

Ferraris, R.P., J.D. Tan and M.C. De La Cruz. – 1987. Development of the digestive tract of milkfish Chanos chanos (Forskal): histology and histochemistry. Aquaculture, 61: 241-257. doi:10.1016/0044-8486(87)90153-0

Gargiulo, A.M., P. Ceccarelli, C. Dall’Aglio and V. Pedini. – 1997. Ultrastructural study on the stomach of Tilapia spp. (Teleostei). Anat. Histol. Embryol., 26: 331-336. doi:10.1111/j.1439-0264.1997.tb00144.x

Goldstein, I.J. and C.E. Hayes. – 1978. The lectins: Carbohydratebinding proteins of plants and animals. Adv. Carbohydr. Chem. Biochem., 35: 127-240. doi:10.1016/S0065-2318(08)60220-6

Grau, A., S. Crespo, M.C. Sarasquete and M.L. Gonzales de Canales. – 1992. The digestive tract of the amberjack Seriola dumerili Risso: a light and scanning electron microscope study. J. Fish. Biol., 41: 287-303. doi:10.1111/j.1095-8649.1992.tb02658.x

Gutierrez, M., C. Sarasquete and M.L. Gonzalez de Canales. – 1986. Distribucion histoquimica de carbohidratos y proteinas en estomago e intestino de Anguilla anguilla L., 1758 (Osteichthyes, Anguillidae) de las Salinas de Cadiz. Invest. Pesq., 50: 553-564.

Harder, W. – 1975. The digestive tract. In: E. Stuttgart (ed.), Anatomy of Fishes, pp. 128-187. Schweizerbart, Stuttgart.

Hellberg, H. and I. Bjerkas. – 2000. The anatomy of the oesophagus, stomach and intestine in common wolffish (Anarhichas lupus): a basis for diagnostic work and research. Acta Vet. Scand., 41: 283-297.

Ihida, K., S. Tsuyama, N. Kashio and F. Murata. – 1991. Subcompartment sugar residues of gastric surface mucous cells studied with labelled lectins. Histochemistry, 95: 329-335. doi:10.1007/BF00266959

Jiang, C., S.F. NcClure, R.W. Stoddart and J. McClure. – 2004. Lectin histochemistry of normal human gastric mucosa. Glycoconj. J., 20: 367-374.

Jirge, S.K. – 1970. Mucopolysaccharide histochemistry of the stomach of fishes with different food habits. Folia Histochem. Cytochem, 8: 275-280.

Kapoor, B.G., H. Smith and I.A. Verighina. – 1975. The alimentary canal and digestion in teleosts. In: F.S. Russel and M. Young (eds.), Adv. Mar. Biol., pp. 109-239. Academic Press, London.

Liquori, G.E., M. Mastrodonato, S. Zizza and D. Ferri. – 2007. Glycoconjugate histochemistry of the digestive tract of Triturus carnifex (Amphibia, Caudata). J. Mol. Histol., 38: 191-199. doi:10.1007/s10735-007-9087-4

Martin, T.J. and S.J.M. Blaber. – 1984. Morphology and histology of the alimentary tracts of Ambassidae (Cuvier) (Teleostei) in relation to feeding. J. Morphol., 182: 295-305. doi:10.1002/jmor.1051820305

Mc Manus, J.F.A. – 1948. Histological and histochemical uses of periodic acid. Stain Technol., 23: 99-108.

Pajak, B. and A. Danguy. – 1993. Characterization of sugar moieties and oligosaccharide sequences in the distal intestinal epithelium of the rainbow trout by means of lectin histochemistry. J. Fish. Biol., 43: 709-722. doi:10.1111/j.1095-8649.1993.tb01148.x

Palazon-Fernandez, J.L., A.M. Arias and C. Sarasquete. – 2001. Aspect of the reproductive biology of the toadfish Halobatrachus didactylus (Schneider, 1801) (Pisces: Batrachoididae). Sci. Mar., 65: 131-138. doi:10.3989/scimar.2001.65n2131

Parillo, F., O. Fagioli and P. Ceccarelli. – 2002. Glucidic determinants expressed by the digestive apparatus of Umbrina cirrosa (L.) fries as revealed by lectin histochemistry. Acta Histochem., 104: 209-215. doi:10.1078/0065-1281-00635

Pearse, A.G.E. – 1968. In: Histochemistry Theoretical and Applied, Vol. I. 3rd edn. Churchill, London.

Pedini, V., P. Scocco, G. Radaelli, O. Fagioli and P. Ceccarelli. – 2001. Carbohydrate hystochemistry of the alimentary canal of the shi drum, Umbrina cirrosa L. Anat. Histol. Embryol., 30: 345-349. doi:10.1046/j.1439-0264.2001.00345.x

Pedini, V., C. Dall’Aglio, F. Parillo and P. Scocco. – 2005. Glycoconjugate distribution in gastric fundic mucosa of Umbrina cirrosa L. revealed by lectin histochemistry. J. Fish Biol., 66: 222-229. doi:10.1111/j.0022-1112.2005.00596.x

Rebolledo, I.M. and J.D Vial. – 1979. Fine structure of the oxynticopeptic cell in the gastric glands of an elasmobranch species (Halaelurus chilensis). Anat. Rec., 193(4): 805-822. doi:10.1002/ar.1091930405

Reid, P.F., C.F. Culing and W.L. Dunn. – 1978. A histochemical method for the identification of 9-O-acyl sialic acids. An investigation of bovine submaximal gland and intestinal mucins. J. Histochem. Cytochem., 26: 187-192.

Reifel, C.W. and A.A Travill. – 1978. Structure and carbohydrate histochemistry of the stomach in eight species of teleosts. J. Morphol., 158: 155-167. doi:10.1002/jmor.1051580203

Rustamova, M.T. and A.A. Khamraev. – 2005. Changes in the content of insoluble glycoproteins in the mucous barrier of the stomach as a diagnostic criterion for associated with Helicobacter infection. Èksp. Klin. Gastroénterol., 5: 43-4, 124.

Sarasquete, C., E. Gisbert, L. Ribeiro, L. Vieira and M.T. Dinis. – 2001. Glycoconjugates in epidermal, branchial and digestive mucous cells and gastric glands of gilthead sea bream, Sparus aurata, Senegal sole, Solea senegalensis and Siberian sturgeon, Acipenser baeri development. Eur. J. Histochem., 45: 267-278.

Scocco, P., G. Menghi and P. Ceccarelli. – 1996. Glycohistochemistry of the Tilapia spp. stomach. J. Fish Biol., 49: 584-593.

Smith, L.S. – 1989. Digestive functions in teleost fishes. In: J.E. Halver (ed.), Fish Nutrition, pp. 331-421. Academic Press, San Diego.

Sommer, U., B. Rehn and M. Kressin. – 2001. Light and electron microscopic investigation of the lectin-binding pattern in the oxyntic gland region of bovine. Ann. Anat., 183: 135-143. doi:10.1016/S0940-9602(01)80033-9

Spicer, S.S., R.G. Horn and T.J. Leppi. – 1967. Histochemistry of connective tissue mucopolysaccharides. In: B.M. Wagner and D.E. Smith (eds.), The Connective tissue, pp. 251-303. Williams and Wilkins, Baltimore, MD.

Spicer, S.S. and B.A. Schulte. – 1992. Diversity of glycoconjugates shown histochemically: a perspective. J. Histochem. Cytochem., 40: 1-38.




How to Cite

Desantis S, Acone F, Zizza S, Deflorio M, Palazón Fernández JL, Sarasquete C, De Metrio G. Glycohistochemical study of the toadfish Halobatrachus didactylus (Scheider, 1801) stomach. scimar [Internet]. 2009Sep.30 [cited 2022Aug.8];73(3):515-2. Available from:




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