Cell cycle analysis of brain cells as a growth index in larval cod at different feeding conditions and temperatures

Rafael González-Quirós; Iyziar Munuera; Arild Folkvord

doi:10.3989/scimar.2007.71n3485

Autores/as

Rafael González-Quirós Departamento de Biología de Organismos y Sistemas (Área de Ecología), Universidad de Oviedo
Iyziar Munuera Departamento de Biología de Organismos y Sistemas (Área de Ecología), Universidad de Oviedo
Arild Folkvord Department of Biology, High Technology Center, University of Bergen, Bergen

DOI:

https://doi.org/10.3989/scimar.2007.71n3485

Palabras clave:

alimento, cerebro, ciclo celular, citometrñia de flujo, índice de crecimiento, larva de bacalao y temperatura

Resumen

El porcentaje de células en divisón en un determinado tejido de una larva de pez se puede estimar analizando la cantidad de ADN por célula mediante citometría de flujo. Se realizó un experimento con larvas de bacalao (Gadus morhua), analizando células de cerebro, para validar esta técnica como índice de crecimiento en larvas de peces. Se analizó la variabilidad de la longitud estándar (SL), la altura del tronco medida en el ano, y el %S (% de células en fase S del ciclo celular), con temperatura (6 y 10ºC), nivel de alimento (alto y sin alimento) y estado de desarrollo larvario (comienzo de la alimentación, pre-metamorfosis y post-metamorfosis) como factores independientes. Las larvas de bacalao crecieron más rápido (en SL) y presentaron mayor %S bajo condiciones de nivel alto de alimento. La SL larvaria incrementó con la temperatura. Sin embargo, se observó una interacción significativa entre temperatura y alimento sobre %S. No hubo diferencias significativas en %S entre 6 y 10ºC en condiciones de nivel alto de alimento. Sugerimos que este resultado es consecuencia de una termo-dependencia en la duración del ciclo celular. En ausencia de alimento, las larvas a 10ºC presentaron %S más bajos que las larvas a 6ºC, lo que puede estar relacionado con un incremento de los costes metabólicos a mayor temperatura. Considerando el efecto de la temperatura, el %S medio explicó el 74% de la variabilidad de la tasa de crecimiento específica estimada.

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Biografía del autor/a

Rafael González-Quirós, Departamento de Biología de Organismos y Sistemas (Área de Ecología), Universidad de Oviedo

Present address: I.F.A.P.A. Centro El Toruño. El Puerto de Santa María. Cádiz

Citas

Aksnes, D.L., C. Troedson and E.M. Thompson. – 2006. A model of developmental time applied to planktonic embryos. Mar. Ecol. Prog. Ser., 318: 75-80.

Bauer, K.D., B. Bagwell, W. Giaretti, M. Melamed, R.J. Zarbo, T.E. Witzig and P.S. Rabinovitch. – 1993. Consensus review of the clinical utility of DNA flow cytometry in colateral cancer. Cytometry, 14: 486-491.

Bergeron, J.-P. – 1997. Nucleic acids in ichthyoplankton ecology: a review, with emphasis on recent advances for new perspectives. J. Fish Biol., 51 (Suppl. A): 284-302.

Bromhead, D., J. Kalish and P. Waring. – 2000. Application of flow cytometry cell cycle analysis to the assessment of condition and growth in larvae of a freshwater teleost Galaxias olidus. Can. J. Fish. Aquat. Sci., 57: 732-741.

Buckley, L. J., E.M. Caldarone and G. Lough. – 2004. Optimum temperature and food-limited growth on larval Atlantic cod (Gadus morhua) and haddock (Melanogrammus aeglefinus) on Georges Bank. Fish. Oceanog., 13: 134-140.

Buckley, L.J., S.I. Turner, T.A. Halavik, A.S. Smigielski, S.M. Drew, and G.C. Laurence. – 1984. Effects of temperature and food availability on growth, survival, and RNA-DNA ratio of larval sand lance (Ammodytes americanus). Mar. Ecol. Prog. Ser., 15: 91-97.

Catalán, I.A., E. Berdalet, M.P. Olivar and C. Roldán. – 2007. Response of muscle-based biochemical condition indices to short-term variations in food availability in post-flexion reared sea bass Dicentrarchus labrax (L.) larvae. J. Fish. Biol. 70: 1-15.

Clemmensen, C. and T. Doan. – 1996. Does otolith structure reflect the nutritional condition of a fish larva? Comparison of otolith structure and biochemical index (RNA/DNA ratio) determined in cod larvae. Mar. Ecol. Prog. Ser., 138: 33-39.

Cooper, G. M. – 2000. The Cell – A Molecular Approach. Sinauer Associates, Sunderland, Massachusetts.

Crossen, P.E. – 1985. The effect of temperature and cell cycle length on SCE frequency in Rat-1 cells. Mutat. Res., 149: 101-104.

Darzynkiewicz, Z., P. Smolewski and E. Bedner. – 2001. Use of flow and laser scanning cytometry to study mechanisms regulating cell cycle and controlling cell death In: J.P. McCoy Jr., and D.F. Keren (eds), New applications of flow cytometry, pp. 857-873. Elsevier Science, Philadelphia, Pennsylvania.

Ellertsen, B., P. Solemdal, T. Strømme, S. Tilseth, T. Westgård and E. Moksness, E. – 1980. Some biological aspects of cod larvae (Gadus morhua L.). Fisk Dir. Skr. Ser. Hav.Unders., 17: 29-47.

Ferron, A. and W.C. Leggett. – 1994. An appraisal of condition measures for marine fish larvae. Adv. Mar. Biol., 30: 216-303.

Finn, R.N., I. Rønnestad, T. van der Meeren and H.J. Fyhn. – 2002. Fuel and metabolic scaling during the early life stages of Atlantic cod Gadus morhua. Mar. Ecol. Prog. Ser., 243: 217-234.

Folkvord, A. – 2005. Comparison of size-at-age of larval cod (Gadus morhua L.) from different populations based on sizeand temperature-dependent models. Can. J. Fish. Aquat. Sci., 62: 1037-1052.

Folkvord, A., K. Rukan, A. Johannessen and E. Moksness. – 1997. Early life history of herring larvae in contrasting feeding environments determined by otolith microstructure analysis. J. Fish Biol., 51: 250-263.

Francis, D. and P.W. Barlow, P.W. – 1988. Temperature and cell cycle. Symp. Soc. Exp. Biol., 42: 181-201.

Galloway, T.F., E. Kjørsvik and H. Kryvi. – 1999. Muscle growth and development in Atlantic cod (Gadus morhua L.) related to different somatic growth rates. J. Exp. Biol., 202: 2111-2120.

García, A., D. Cortés, T. Ramírez, C. Guisande, J. Quintanilla, F. Alemany, J.M. Rodríguez, J.P. Álvarez and Á. Carpena. – 2007. Field comparison of sardine post-flexion larval growth and biochemical composition from three sites in the W Mediterranean (Ebro river coast, bays of Almería and Málaga). Sci. Mar., 70S2: 79-91.

Hedley, D.W., T.V. Shankey and L.L. Wheeless. – 1993. DNA Cytometry Consensus Conference. Cytometry 14: 471.

Høie, H., A. Folkvord and E. Otterlei. – 2003. Effect of somatic and otolith growth rate on stable isotopic composition of early juvenile cod (Gadus morhua) otoliths. J. Exp. Mar. Biol. Ecol., 289: 41-58.

Houde, E.D. – 1987. Fish early life dynamics and recruitment variability. Am. Fish. Soc. Symp. 2: 17-29.

Houde, E.D. – 1989. Comparative growth, mortality and energetics of marine fish larvae: temperature and implied latitudinal effects. Fish. Bull. US, 87: 471-495.

Houde, E.D. – 1997. Patterns and trends in larval-stage growth and mortality of teleost fish. J. Fish Biol., 51(Suppl. A): 52-83.

Leggett, W.C. and E. Deblois. – 1994. Recruitment in marine fishes: is it regulated by starvation and predation in the egg and larval stages? Neth. J. Sea Res., 32: 119-134.

Otterlei, E., G. Nyhammer, A. Folkvord and S.O. Stefansson. – 1999. Temperature- and size-dependent growth of larval and early juvenile Atlantic cod (Gadus morhua): a comparative study of Norwegian coastal cod and northeast Arctic cod. Can. J. Fish. Aquat. Sci., 56: 2099-2111.

Pepin, P. – 1991. Effect of temperature and size on development, mortality, and suavival rates of the pelagic early life history stages of marine fish. Can. J. Fish. Aquat. Sci., 48: 503-518.

Pepin, P. and R.A. Myers. – 1991. Significance of egg and larval size to recruitment variability of temperate marine fish. Can. J. Fish. Aquat. Sci., 48: 1820-1828.

Shankey, T.V., P.S. Rabinovitch, B. Bagwell, K.D. Bauer, R.E. Duque, D.W. Hedley, B.H. Mayall and L.L. Wheeless. – 1993. Guidelines for implementation of clinical DNA cytometry. Cytometry, 14: 472-477.

Theilacker, G.H. and W. Shen. – 1993a. Calibrating starvationinduced stress in larval fish using flow cytometry. Am. Fish. Soc. Symp., 14: 85-94.

Theilacker, G.H. and W. Shen. – 1993b. Fish larval condition analyzed using flow cytometry. In: B.T. Walter. and H.J. Fyhn (Eds), Physiological and biochemical aspects of fish development, pp. 346-355. University of Bergen, Norway.

Theilacker, G.H. and W. Shen. – 2001. Evaluating growth of larval walleye pollock, Theragra chalcogramma, using cell cycle analysis. Mar. Biol., 138: 897-907.

Theilacker, G.H., K.M. Bailey, M.F. Canino and S.M. Porter. – 1996. Variations in larval walleye pollock feeding and condition: a synthesis. Fish. Oceanog., 5(Suppl 1): 112-123. Underwood, A.J. – 1997. Experiments in Ecology. Cambridge University Press. Cambrige. 504 pp.

Yamashita, M. – 2003. Apoptosis in zebrafish development. Comp. Biochem. Physiol. B-Biochem Mol. Biol. 136: 731-742. van der Meeren, T. – 1991. Selective feeding and prediction of food consumption in turbot larvae (Scophthalmus maximus L.) reared on the rotifer Brachionus plicatilis and natural zooplankton. Aquaculture, 93: 35-55.

West, J., M.J. Lyttleton and F. Gianelli. – 1981. Effect of incubation temperature on the frequency of sister cromatid exchanges in Bloom’s syndrome lymphocites. Hum. Genet., 59: 204-207.

Westerman, M.E., G.J. Holt. and L. DiMichele. – 1999. Quantitative assy of cyclin-dependent kinase activity as a sensitive marker of cell proliferation in marine teleost larvae. Mar. Biotechnol., 1: 297-310.