Effects of the toxic dinoflagellate Karlodinium sp. (cultured at different N/P ratios) on micro and mesozooplankton
DOI:
https://doi.org/10.3989/scimar.2006.70n159Keywords:
micro- and mesozooplankton (Oxyrrhis marina and Acartia margalefi), toxic and non-toxic dinoflagellate (Karlodinium sp., Gymnodinium sp1) grazing rates, P-limitation, predator mortality, Karlodinium sp., egg viability.Abstract
An experimental study was carried out to investigate whether two potential predators such as Oxyrrhis marina (phagotrophic dinoflagellate) and Acartia margalefi (Copepoda: Calanoida) had different responses when feeding on toxic (Karlodinium sp.-strain CSIC1-) or non-toxic (Gymnodinium sp1) dinoflagellates with a similar shape and size. Both prey were cultured at different N/P ratios (balanced N/P = 15, and P-limited N/P > 15) to test whether P-limitation conditions could lead to depressed grazing rates or have other effects on the predators. Both predators ingested the non-toxic Gymnodinium sp1, and low or non-ingestion rates were observed when incubated with Karlodinium sp. The dinoflagellate O. marina did not graze at all on Karlodinium sp. at N/P > 15 and very little at NP = 15, as its net growth rates were always negative when feeding on Karlodinium sp. cultured under P-limitation conditions. A. margalefi had lower ingestion rates when feeding on Karlodinium sp. grown at N/P = 15 than when feeding on Gymnodinium sp1, and did not graze on P-limited Karlodinium sp. Nevertheless, feeding on Karlodinium sp. grown under N/P =15 or N/P > 15 did not have any paralyzing or lethal effect on A. margalefi after 24 h. Finally, a direct effect on the viability of A. margalefi eggs was detected when healthy eggs were incubated for 5 days in the presence of Karlodinium sp. grown under N/P =15 or N/P > 15, producing a decrease in viability of 20% and 60% respectively.
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Anderson, DM., P.T.O Cheng, J.J. Sullivan, S. Hall and C. Lee. –1990. Dynamics and physiology of saxitoxin production by the dinoflagellate Alexandrium minutum spp. Mar. Biol., 104: 511-524. doi:10.1007/BF01314358
Boyer, G.L., J.J. Sullivan, R.J. Anderson, P.J. Harrison and F.J.R.Taylor. – 1987. Effects of nutrient limitation on toxic production and composition in the marine dinoflagellate Protogonyaulax tamarensis. Mar. Biol., 96: 123-128. doi:10.1007/BF00394845
Burkholder, J.M., H.B. Glasgow and N. Deamer-Melia, – 2001. Overview and present status of the toxic Pfiesteria complex (Dinophyceae). Phycologia, 40: 186-214.
Calbet, A., D. Vaqué, J. Felipe, M. Vila, M.M. Sala, M. Alcaraz and M. Estrada. – 2003. Relative grazing impact of microzooplankton and mesozooplankton on a bloom of the toxic dinoflagellate Alexandrium minutum. Mar. Ecol. Prog. Ser., 259: 303-309. doi:10.3354/meps259303
Camp, J., E. Flo and M. Masó. – 2003. Monitoring program of quality control of water littoral waters. Final report. Compilation of results 1994-2001. Catalan water Society and Institut of Marine Sciences (CMIMA-CSIC). 127 pp.
Cembella, A.D. and J.C. Therriault. – 1998. Comparative toxicity of cultured isolates and natural populations of Protogonyaulax tamarensis (Lebour) Taylor from the St. Lawrence. J. Shellfish Res., 7: 152.
Deeds, J.R., D.E. Terlizzi, J.E. Adolf, D.K Stoecker and A.R. Place.– 2002. Toxic activity from cultures of Karlodinium micrum (=Gyrodinium galatheanum) (Dynophyceae)-a dinoflagellate associated with fish mortalities in an estuarine aquaculture facility. Harmful Algae., 21: 169-189. doi:10.1016/S1568-9883(02)00027-6
Deeds, J.R., J.P.Y. Kao, R.E. Hoesch and A.R. Place. – 2003. Toxic mode of action of KmxT2, a new fish-killing toxin from Karlodinium micrum (Dinophyceae). Second Symposium on Harmful Marine Algae. Woods Hole Oceanographic Institution (USA). December 9-13. Abstracts book: 6p.
Delgado, M., J.V. Fernandez, E. Garcés, E. Matamoros and J. Camp. – 1995. Blooms of a dinoflagellate belong to the genus Gyrodinium in Alfacs Bay (Ebro Delta), associated to fish mortalities. Proccedings of the Fifth National Congress of Aquaculture. Eds: Castelló, F. and A. Calderer. San Carles de la Ràpita. 10-13 May 1995.
Delgado, M. and M. Alcaraz. – 1999. Interactions between red tide microalgae and herbivorous zooplankton: the noxious effects of Gyrodinium corsicum (Dinophyceae) on Acartia grani (Copepoda: Calanoida). J. Plankton Res., 21: 2361-2371. doi:10.1093/plankt/21.12.2361
Fernandez-Tejedor, M., E. Garcés, J. Camp, A. Penna and M. Zapata. – 2003. Karlodinium micrum (=Gyrodinium galatheanum) an ichthyotoxic dinoflagellate in Alfacs bay. Plankton Symposium. Vigo (Spain). October 9-13.
Fernandez-Tejedor, M., M.A. Soubrier-Pedreño, P. Riobó, and E. Cacho. – 2003. Toxicidad “in vivo” e “in vitro” de los cultivos de Gyrodinium corsicum. VIII Reunión Iberica sobre Fitoplancton tóxico y biotoxinas. Universidad de La Laguna (2003).
Fistarol, G.O., C. Legrand, E. Selander, C. Hummert, W. Soilte and E. Graneli. – 2004. Allelopathy in Alexandrium spp.: effect on a natural plankton community and on algal monocultures. Aquat. Microb. Ecol., 35: 45-56. doi:10.3354/ame035045
Garcés, E., M. Masó, J. Camp. – 1999. A recurrent and localized dinoflagellate bloom in a Mediterranean beach. J. Plankton Res., 21: 2373-2391. doi:10.1093/plankt/21.12.2373
Gentien, P., G. Arzul, and F. Toularastel. – 1991. Modes of action of the toxic principle of Gyrodinium aureolum. Proccedings of Symposim on marine biotoxins, Paris, 30-31 January 1991. Actes du Colloque sur les Biotoxins Marines, pp. 83-86.
Guisande, C., M. Frangópulos, I. Maneiro, A.R. Vergara and I. Riveiro. – 2002. Ecological advantages of toxin production by the dinoflagellate Alexandrium minutum under phosphorous limitation. Mar. Ecol. Prog. Ser., 225: 169-178. doi:10.3354/meps225169
Johnson, M.D., M. Rome and D.K. Stoecker. – 2003. Microzooplankton grazing on Prorocentrum minimum and Karlodinium micrum in Chesapeake Bay. Limnol. Oceanogr., 48: 238-248.
Olivos, A. – 2000. Dissolved inorganic nutrients in littoral waters of the Catalan Sea. PhD. Thesis, 134 pp. University of Barcelona.
Smayda T.J. – 1997. Harmful algal blooms: their ecophysiology and general relevance to phytoplankton bloom in the sea. Limnol. Oceanogr., 42: 1137-1153.
Suikkanen, S., G.O. Fistarol, and E. Granéli. – 2004. Allelopathic effects of the Baltic Cyanobacteria Nodularia spumigena, Aphanizomenon flos-aquae and Anabaena Lemmermannii on algal monocultures. J. Exp. Mar. Biol. Ecol., 308: 85-101. doi:10.1016/j.jembe.2004.02.012
Teegarden G.J. – 1999 Copepod grazing selection and particle discrimination on the basis of PSP toxin content. Mar. Ecol. Prog. Ser., 181: 163-176. doi:10.3354/meps181163
Turner, J.T. and D.M. Anderson. – 1983. Zooplankton grazing during dinoflagellate blooms in Cape Cod embayment with observations of predation upon tintinnids by copepods. PSZN I: Mar. Ecol., 4: 358-374.
Turner, J.A. and P.A.Tester. – 1997. Toxic marine phytoplanktpn, zooplankton grazers and pelagic food webs. Limnol. Oceanogr., 42: 1203-1214.
Uye, S. – 1986. Impact of copepod grazing on the red tide flagellate Chatonella antiqua. Mar Biol., 95: 35-43. doi:10.1007/BF00392743
Vaqué, D., J. Felipe and M. Alcaraz. – 2003. Effects of a toxic dinoflagellate on planktonic predators: repressed feeding and production rates on meso-and microzooplankton. Abstracts of EUROHAB meeting. Amsterdam March 2003.
Vila, M., J. Camp, E. Garcés, M. Masó and M. Delgado. – 2001. High resolution spatio-temporal detection of potentially harmful dinoflagellates in confined waters of the NW Mediterranean. J. Plankton Res., 23: 497-514. doi:10.1093/plankt/23.5.497
Vila, M. and M. Masó. – 2005. Phytoplankton functional groups and harmful algal species in antropogenically impacted waters of the NW Mediterranean Sea. Sci. Mar., 69: 31-45.
Watras, C.J., V.C. Garcon, R.J. Olson, S.W. Chisholm and D.M. Anderson. – 1985. The effect of zooplankton on estuarine blooms of the toxic dinoflagellate Gonyaulax tamarensis. J. Plankton Res., 7: 891-908. doi:10.1093/plankt/7.6.891
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