Microplastics in wild mussels (Mytilus spp.) from the north coast of Spain





biota samples, NW Spain, seafood, biomonitoring, bivalve molluscs, microplastics


Microplastic content (MPs) in mussels (Mytilus spp.) from two areas of the north coast of Spain was measured for the first time. Additionally, a comparison of microplastic levels observed in mussels digested with nitric acid and with potassium hydroxide was carried out. The average microplastic concentration in mussels digested with nitric acid was significantly lower than that observed in mussels digested with potassium hydroxide (p < 0.05). The average concentration of microplastics in mussels from the Cantabrian Sea (2.55±2.80 MPs g–1 WW) was slightly higher than that in mussels from the Ria of Vigo (1.59±1.28 MPs g–1 WW). Both in the Ria of Vigo and in the Cantabrian Sea the observed pattern of pollution was fitted to the one expected. Consequently, mussels have been confirmed as suitable sentinel organisms for microplastic pollution.


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Arthur C., Baker J., Bamford H. (eds). 2009. Proceedings of the International Research Workshop on the Occurrence, Effects, and Fate of Microplastic Marine Debris. 9-11 Sept. 2008. NOAA Technical Memorandum NOS-OR&R-30, Silver Spring, 49 pp.

Amélineau F., Bonnet D., Heitz O., et al. 2016. Microplastic pollution in the Greenland Sea: Background levels and selective contamination of planktivorous diving seabirds. Environ. Pollut. 219: 1131-1139. https://doi.org/10.1016/j.envpol.2016.09.017 PMid:27616650

Andrady A.L., Neal M.A. 2009. Applications and societal benefits of plastics. Philos. Trans. R. Soc. B 364: 1977-1984. https://doi.org/10.1098/rstb.2008.0304 PMid:19528050 PMCid:PMC2873019

Auta H.S., Emenike C.U., Fauziah S.H. 2017. Distribution and importance of microplastics in the marine environment: A review of the sources, fate, effects, and potential solutions. Environ. Int. 102: 165-176. https://doi.org/10.1016/j.envint.2017.02.013 PMid:28284818

Beecham J. 2008. A literature review on particle assimilation by molluscs and crustaceans. Report Nº: RL 10/08. Cefas Environment Report. Centre for Environment, Fisheries and Aquaculture Science, Lowestoft, 17 pp.

Bellas J., Albentosa M., Vidal-Liñán L., et al. 2014. Combined use of chemical, biochemical and physiological variables in mussels for the assessment of marine pollution along the N-NW Spanish coast. Mar. Environ. Res. 96: 105-117. https://doi.org/10.1016/j.marenvres.2013.09.015 PMid:24119442

Besada V., Sericano J.L., Schultze F. 2014. An assessment of two decades of trace metals monitoring in wild mussels from the Northwest Atlantic and Cantabrian coastal areas of Spain, 1991- 2011. Environ. Int. 71: 1-12. https://doi.org/10.1016/j.envint.2014.05.024 PMid:24946271

Beyer J., Green N.W., Brooks S., et al. 2017. Blue mussels (Mytilus edulis spp.) as sentinel organisms in coastal pollution monitoring: A review. Mar. Environ. Res. 130: 338-365. https://doi.org/10.1016/j.marenvres.2017.07.024 PMid:28802590

Bråte I.L.N., Hurley R., Iversen K., et al. 2018. Mytilus spp. as sentinels for monitoring microplastic pollution in Norwegian coastal waters: A qualitative and quantitative study. Environ. Pollut. 243: 383-393. https://doi.org/10.1016/j.envpol.2018.08.077 PMid:30212794

Browne M.A., Dissanayake A., Galloway T.S., et al. 2008. Ingested microscopic plastic translocates to the circulatory system of the mussel, Mytilus edulis (L.). Environ. Sci. Technol. 42: 5026-5031. https://doi.org/10.1021/es800249a PMid:18678044

Catarino A.I., Macchia V., Sanderson W.G., et al. 2018. Low levels of microplastics (MP) in wild mussels indicate that MP ingestion by humans is minimal compared to exposure via household fibres fallout during a meal. Environ. Pollut. 237: 675-684. https://doi.org/10.1016/j.envpol.2018.02.069 PMid:29604577

Claessens M., Van Cauwenberghe L., Vandegehuchte M.B., et al. 2013. New techniques for the detection of microplastics in sediments and field collected organisms. Mar. Pollut. Bull. 70: 227-233. https://doi.org/10.1016/j.marpolbul.2013.03.009 PMid:23601693

De Witte B., Devriese L., Bekaert K., et al. 2014. Quality assessment of the blue mussel (Mytilus edulis): Comparison between commercial and wild types. Mar. Pollut. Bull. 85: 146-155. https://doi.org/10.1016/j.marpolbul.2014.06.006 PMid:24969855

Dehaut A., Cassone A.-L., Frère L., et al. 2016. Microplastics in seafood: Benchmark protocol for their extraction and characterization. Environ. Pollut. 215: 223-233. https://doi.org/10.1016/j.envpol.2016.05.018 PMid:27209243

Desforges J.-P.W., Galbraith M., Ross P.S. 2015. Ingestion of microplastics by zooplankton in the Northeast Pacific Ocean. Arch. Environ. Contam. Toxicol. 69: 320-330. https://doi.org/10.1007/s00244-015-0172-5 PMid:26066061

Díez I., Secilla A., et al. 2000. The north coast of Spain. In: Sheppard C.R.C. (ed), Seas at the Millennium: an Environmental Evaluation. I. Regional chapters: Europe, the Americas and West Africa. Pergamon, Amsterdam, pp. 135-150.

Digka N., Tsangaris C., Torre M., et al. 2018. Microplastics in mussels and fish from the Northern Ionian Sea. Mar. Pollut. Bull. 135: 30-40. https://doi.org/10.1016/j.marpolbul.2018.06.063 PMid:30301041

Eriksen M., Maximenko N., Thiel M., et al. 2013. Plastic pollution in the South Pacific subtropical gyre. Mar. Pollut. Bull. 68: 71-76. https://doi.org/10.1016/j.marpolbul.2012.12.021 PMid:23324543

Fernández E., Álvarez-Salgado X.A., Beiras R., et al. 2016. Coexistence of urban uses and shellfish production in an upwelling-driven, highly productive marine environment: The case of the Ría de Vigo (Galicia, Spain). Reg. Stud. Mar. Sci. 8: 362-370. https://doi.org/10.1016/j.rsma.2016.04.002

Foekema E.M., De Gruijter C., Mergia M.T., et al. 2013. Plastic in North Sea fish. Environ. Sci. Technol. 47: 8818-8824. https://doi.org/10.1021/es400931b PMid:23777286

Gago J., Carretero O., Filgueiras A.V., et al. 2018. Synthetic microfibers in the marine environment: A review on their occurrence in seawater and sediments. Mar. Pollut. Bull. 127: 365-376. https://doi.org/10.1016/j.marpolbul.2017.11.070 PMid:29475673

Galgani F., Hanke G., Werner S., et al. 2013. Guidance on monitoring of marine litter in European seas. Report Nº: EUR 26113 EN. JRC Scientific and Policy Reports. Joint Research Centre of the European Commission, Luxembourg, 124 pp.

Graham E.R., Thompson J.T. 2009. Deposit- and suspension-feeding sea cucumbers (Echinodermata) ingest plastic fragments. J. Exp. Mar. Biol. Ecol. 368: 22-29. https://doi.org/10.1016/j.jembe.2008.09.007

Hidalgo-Ruz V., Gutow L., Thompson R.C., et al. 2012. Microplastics in the marine environment: A review of the methods used for identification and quantification. Environ. Sci. Technol. 46: 3060-3075. https://doi.org/10.1021/es2031505 PMid:22321064

Ivar do Sul J.A., Costa M.F. 2014. The present and future of microplastic pollution in the marine environment. Environ. Pollut. 185: 352-364. https://doi.org/10.1016/j.envpol.2013.10.036 PMid:24275078

Jambeck J.R., Geyer R., Wilcox C., et al. 2015. Plastic waste inputs from land into the ocean. Science 347: 768-771. https://doi.org/10.1126/science.1260352 PMid:25678662

Karami A., Golieskardi A., Choo C.K., et al. 2017. A high-performance protocol for extraction of microplastics in fish. Sci. Total Environ. 578: 485-494. https://doi.org/10.1016/j.scitotenv.2016.10.213 PMid:27836345

Karlsson T.M., Vethaak A.D., Almroth B.C., et al. 2017. Screening for microplastics in sediment, water, marine invertebrates and fish: Method development and microplastic accumulation. Mar. Pollut. Bull. 122: 403-408. https://doi.org/10.1016/j.marpolbul.2017.06.081 PMid:28689849

Labarta U., Corbacho E.P. 2002. La industria del mejillón: mercados internacionales, productos y países. Documentos de Economía, 13. Fundación Caixa Galicia, Santiago de Compostela, 66 pp.

Law K.L., Morét-Ferguson S., Maximenko N.A., et al. 2010. Plastic accumulation in the North Atlantic subtropical gyre. Science 329: 1185-1188. https://doi.org/10.1126/science.1192321 PMid:20724586

Leslie H.A., Brandsma S.H., van Velzen M.J.M., et al. 2017. Microplastics en route: Field measurements in the Dutch river delta and Amsterdam canals, wastewater treatment plants, North Sea sediments and biota. Environ. Int. 101: 133-142. https://doi.org/10.1016/j.envint.2017.01.018 PMid:28143645

Li J., Qu X., Su L., et al. 2016. Microplastics in mussels along the coastal waters of China. Environ. Pollut. 214: 177-184. https://doi.org/10.1016/j.envpol.2016.04.012 PMid:27086073

Li J., Green C., Reynolds A., et al. 2018. Microplastics in mussels sampled from coastal waters and supermarkets in the United Kingdom. Environ. Pollut. 241: 35-44. https://doi.org/10.1016/j.envpol.2018.05.038 PMid:29793106

Li J., Lusher A.L., Rotchell J.M., et al. 2019. Using mussel as a global bioindicator of coastal microplastic pollution. Environ. Pollut. 244: 522-533. https://doi.org/10.1016/j.envpol.2018.10.032 PMid:30368156

Little C., Kitching J.A. 1996. The Biology of Rocky Shores. Oxford University Press, New York, 240 pp.

Lusher A.L., Hernandez-Milian G., O'Brien J., et al. 2015. Microplastic and macroplastic ingestion by a deep diving, oceanic cetacean: The True's beaked whale Mesoplodon mirus. Environ. Pollut. 199: 185-191. https://doi.org/10.1016/j.envpol.2015.01.023 PMid:25667115

Munno K., Helm P.A., Jackson D.A., et al. 2018. Impacts of temperature and selected chemical digestion methods on microplastic particles. Environ. Toxicol. Chem. 37: 91-98. https://doi.org/10.1002/etc.3935 PMid:28782833

Newman M.C. 2010. Fundamentals of Ecotoxicology. CRC Press, Boca Raton, 541 pp.

OSPAR. 2012. JAMP guidelines for monitoring contaminants in biota (Agreement 1999-02. Revised 2012). OSPAR Commission, London, 122 pp.

Phuong N.N., Poirier L., Pham Q.T., et al. 2018. Factors influencing the microplastic contamination of bivalves from the French Atlantic coast: Location, season and/or mode of life? Mar. Pollut. Bull. 129: 664-674. https://doi.org/10.1016/j.marpolbul.2017.10.054 PMid:29106937

Qu X., Su L., Li H., et al. 2018. Assessing the relationship between the abundance and properties of microplastics in water and in mussels. Sci. Total Environ. 621: 679-686. https://doi.org/10.1016/j.scitotenv.2017.11.284 PMid:29197287

Railo S., Talvitie J., Setälä O., et al. 2018. Application of an enzyme digestion method reveals microlitter in Mytilus trossulus at a wastewater discharge area. Mar. Pollut. Bull. 130: 206-214. https://doi.org/10.1016/j.marpolbul.2018.03.022 PMid:29866549

Rosón G., Cabanas J.M., Pérez F.F. 2008. Hidrografía y dinámica de la Ría de Vigo. Un sistema de afloramiento. In: González- Garcés A., Vilas F., Alvárez-Salgado X.A. (eds), La Ría de Vigo. Una aproximación integral al ecosistema marino de la Ría de Vigo. Instituto de Estudios Vigueses, Vigo, pp. 51-84.

R Core Team. 2017. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna. URL: https://www.r-project.org/.

Van Cauwenberghe L., Claessens M., Vandegehuchte M.B., et al. 2015. Microplastics are taken up by mussels (Mytilus edulis) and lugworms (Arenicola marina) living in natural habitats. Environ. Pollut. 199: 10-17. https://doi.org/10.1016/j.envpol.2015.01.008 PMid:25617854

Vandermeersch G., Van Cauwenberghe L., Janssen C.R., et al. 2015. A critical view on microplastic quantification in aquatic organisms. Environ. Res. 143: 46-55. https://doi.org/10.1016/j.envres.2015.07.016 PMid:26249746

von Moos N., Burkhardt-Holm P., Köhler A. 2012. Uptake and effects of microplastics on cells and tissue of the blue mussel Mytilus edulis L. after an experimental exposure. Environ. Sci. Technol. 46: 11327-11335. https://doi.org/10.1021/es302332w PMid:22963286

Wright S.L., Thompson R.C., Galloway T.S. 2013. The physical impacts of microplastics on marine organisms: A review. Environ. Pollut. 178: 483-492. https://doi.org/10.1016/j.envpol.2013.02.031 PMid:23545014

Ziccardi L.M., Edgington A., Hentz K., et al. 2016. Microplastics as vectors for bioaccumulation of hydrophobic organic chemicals in the marine environment: A state-of-the-science review. Environ. Toxicol. Chem. 35: 1667-1676. https://doi.org/10.1002/etc.3461 PMid:27093569



How to Cite

Reguera P, Viñas L, Gago J. Microplastics in wild mussels (Mytilus spp.) from the north coast of Spain. Sci. mar. [Internet]. 2019Dec.30 [cited 2024Feb.29];83(4):337-4. Available from: https://scientiamarina.revistas.csic.es/index.php/scientiamarina/article/view/1822




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