The use of energy dispersive X-ray spectroscopy to detect strontium marks in fish otoliths
Keywords:otolith fingerprinting, marking, Sr, mortality
Otolith marking provides a reference point for otolith growth patterns by validating the temporal significance of growth increments. This widespread method is primarily implemented using fluorescent dyes. The incorporation of a trace element that appears naturally in otoliths offers an alternative approach. Diplodus annularis and Serranus scriba otoliths were marked with an intramuscular injection of SrCl2 diluted in 0.9% sterile saline solution (55 mg Sr ml-1 saline solution), given at a dose of 100 mg Sr kg-1 fish. At 277 to 366 days after marking, the fishes showed little or no mortality and experienced growth in length and weight. All of the otoliths analyzed showed a clear Sr mark detected with backscattered or secondary electron imaging during a scanning electron microsope (SEM) analysis. The mark was confirmed by Sr analysis using an X-ray detector and an energy dispersive spectrometer system with the SEM. The otoliths continued to grow after the mark, and background Sr values in this new growth were much lower than at the mark. This method was feasible and yielded good results. However, the method’s cost may limit its applicability to experimental studies.
Campana S.E. 1999. Chemistry and composition of fish otoliths: pathways, mechanisms and applications. Mar. Ecol. Prog. Ser. 188: 263-297. http://dx.doi.org/10.3354/meps188263
Casselman J.M. 1982. Chemical analyses of the optically different zones in eel otoliths. Ont. Fish. Tech. Rep. Ser. 4: 74-82.
Chang C.W., Lin S.H., Iizuka Y., Tzeng W.N. 2004. Relationship between Sr:Ca ratios in otoliths of grey mullet Mugil cephalus and ambient salinity: Validation, mechanisms, and applications. Zool. Stud. 43: 74-85.
Elsdon T.S., Gillanders B.M. 2003. Reconstructing migratory patterns of fish based on environmental influences on otolith chemistry. Rev. Fish. Biol. Fish. 13: 219-235. http://dx.doi.org/10.1023/B:RFBF.0000033071.73952.40
Gibson S.M., Patterson W.F., Phelps R.P., Patterson W.P., Chen Z.X. 2010. Distinguishing wild from hatchery-produced juvenile red snapper with otolith chemical signatures. North. Am. J. Fish. Manage. 30: 1176-1186. http://dx.doi.org/10.1577/M10-085.1
Kennedy B.P., Blum J.D., Folt C.L., Nislow K.H. 2000. Using natural strontium isotopic signatures as fish markers: methodology and application. Can. J. Fish. Aquat. Sci. 57: 2280-2292. http://dx.doi.org/10.1139/f00-206
Kuroki M., Buckley R.M., LeClair L.L., Hauser L. 2010. Validation and efficacy of transgenerational mass marking of otoliths in viviparous fish larvae. J. Fish. Biol. 77: 292-298. http://dx.doi.org/10.1111/j.1095-8649.2010.02681.x PMid:20646154
Morales-Nin B., Grau A., Pérez-Mayol S., Pastor E., Palmer M. 2011. Oxytetracycline hydrochloride vital labelling revisited: the case of Dicentrarchus labrax and Diplodus puntazzo. J. Fish. Biol. 78: 762-782 http://dx.doi.org/10.1111/j.1095-8649.2010.02887.x PMid:21366571
Munro A.R., Gillanders, B.M., Elsdon T.S., Crook D.A., Sanger A.C. 2008. Enriched stable isotope marking of juvenile golden perch (Macquaria ambigua) otoliths. Can. J. Fish. Aquat. Sci. 65: 276-285. http://dx.doi.org/10.1139/f08-010
Payan P., Edeyer A., De Pontual H., Borelli G., Boeuf G., Mayer-Gostan N. 1999. Chemical composition of saccular endolymph and otolith in fish inner ear: lack of spatial uniformity. Am. J. Physiol.-Regu. Physiol. 277: R123-R131.
Phillis C.C., Ostrach D.J., Ingram B.L., Weber P.K. 2011. Evaluating otolith Sr/Ca as a tool for reconstructing estuarine habitat use. Can. J. Fish. Aquat. Sci. 68: 360-373. http://dx.doi.org/10.1139/F10-152
Tanner S.E., Vasconcelos R.P., Reis-Santos P., Cabral H.N., Thorrold S.R. 2011. Spatial and ontogenetic variability in the chemical composition of juvenile common sole (Solea solea) otoliths. Est. Coast. Shelf Sci. 91: 150-157. http://dx.doi.org/10.1016/j.ecss.2010.10.008
Tzeng W.N., Severin K.P., Wickstrom H. 1997. Use of otolith microchemistry to investigate the environmental history of European eel Anguilla anguilla. Mar. Ecol. Prog. Ser. 149: 73-81. http://dx.doi.org/10.3354/meps149073
Wright P.J., Woodroffe D.A., Gibb F.M., Gordon J.D.M. 2002. Verification of first annulus formation in the illicia and otoliths of white anglerfish, Lophius piscatorius using otolith microstructure. ICES J. Mar. Sci. 59:587-593. http://dx.doi.org/10.1006/jmsc.2002.1179
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