Scientia Marina, Vol 78, No 4 (2014)

The nematode assemblage of a coastal lagoon (Lake Varano, southern Italy): ecology and biodiversity patterns

Federica Semprucci
Dipartimento di Scienze della Terra, della Vita e dell’Ambiente (DiSTeVA), Università di Urbino , Italy

Maria Balsamo
Dipartimento di Scienze della Terra, della Vita e dell’Ambiente (DiSTeVA), Università di Urbino , Italy

Fabrizio Frontalini
Dipartimento di Scienze della Terra, della Vita e dell’Ambiente (DiSTeVA), Università di Urbino , Italy


This study was conducted in Lake Varano (southern Italy) in order to determine the environmental parameters that influence nematode taxonomic and functional diversity and how they influence them, and to classify for the first time the ecological quality status of a transitional environment. The general composition and level of taxonomic diversity were comparable with those reported for transitional environments worldwide. Principal component analysis revealed that the main environmental factors controlling the assemblages in terms of both composition and biological traits were grain size, organic matter, pollution load index and, only secondarily, salinity. Molgolaimus allgeni, Terschellingia longicaudata and Leptolaimus luridus showed higher values in relation to silt, organic matter and pollution load index, while Axonolaimus caudostriatus, Odontophora wieseri, Paramonhystera pellucida and Paracanthonchus longicaudatus showed higher values in relation to the redox potential and sand percentage. Salinity was detected as an influencing parameter only secondarily and particularly for Calomicrolaimus honestus, Daptonema normandicum, Thalassomonhystera parva and Sabatieria pulchra. With respect to functional traits, the trophic groups seemed to be mainly related to sediment type, which is in turn reflected by the nature of the available food. The maturity index, as well as c-p classes, did not always permit a clear interpretation of the interaction between anthropogenic and natural factors. However, the greater influence of the San Antonino and San Francesco Canals was perfectly revealed by the nematode assemblage. In accordance with the current ecological quality classes, Lake Varano can mainly be classified as having good to poor ecological quality status.


free-living nematodes; biodiversity; transitional environment; ecological quality; Lake Varano

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Airoldi L., Beck M.W. 2007. Loss, status and trends for coastal marine habitats of Europe. Oceanogr. Mar. Biol. Annu. Rev. 45: 345-405.

Albertelli G., Covazzi-Harriague A., Danovaro R., et al. 1999. Differential responses of bacteria, meiofauna and macrofauna in a shelf area (Ligurian Sea, NW Mediterranean): role of food availability. J. Sea. Res. 42: 11-26.

Appeltans W. et al. 2012. The Magnitude of Global Marine Species Diversity. Curr. Biol. 22: 2189-2202. PMid:23159596

Armenteros M., Ruiz-Abierno A., Fernández-Garce R., et al. 2009. Biodiversity patterns of free-living marine nematodes in a tropical bay: Cienfuegos, Caribbean Sea. Estuar. Coast. Shelf. Sci. 85: 179-189.

Armynot du Châtelet E., Debenay J.P., Soulard R. 2004. Foraminiferal proxies for pollution monitoring in moderately polluted harbours. Environ. Pollut. 127: 27-40.

Armynot du Châtelet E., Bout-Roumazeilles V., Coccioni R., et al. 2013. Environmental control on shell structure and composition of agglutinated foraminifera along a proximal-distal transect in the Marmara Sea. Mar. Geol. 335: 114-128.

Balsamo M., Albertelli G., Ceccherelli V.U., et al. 2010. Meiofauna of the Adriatic Sea: current state of knowledge and future perspective. Chem. Ecol. 26: 45-63.

Balsamo M., Semprucci F., Frontalini F., et al. 2012. Meiofauna as a tool for marine ecosystem biomonitoring. In: Cruzado A. (ed.), Marine Ecosystems. In Tech Publisher: pp. 77-104.

Barnes N., Bamber R.N., Moncrieff C.B., et al. 2008. Meiofauna in closed coastal saline lagoons in the United Kingdom: Structure and biodiversity of the nematode assemblage. Estuar. Coast. Shelf. Sci. 79: 328-340.

Belmonte G., Scirocco T., Denitto F. 2011. Zooplankton composition in Lake Varano (Adriatic Sea coast, Italy). Ital. J. Zool. 78: 370-378.

Beneduce L., Vernile A., Spano G., et al. 2010. Occurrence of Vibrio vulnificus in mussel farms from the Varano lagoon environment. Lett. Appl. Microbiol. 51: 443-449. PMid:20807335

Bernardello M., Secco T., Pellizzato F., et al. 2006. The changing state of contamination in the lagoon of Venice. Part 2: heavy metals. Chemosphere 64: 1334-1345. PMid:16469357

Bongers T. 1990. The maturity index: an ecological measure of environmental disturbance based on nematode species composition. Oecologia 83: 14-19.

Bongers T., Alkemade R., Yeates G.W. 1991. Interpretation of disturbance-induced maturity decrease in marine nematode assemblages by means of the Maturity Index. Mar. Ecol. Prog. Ser. 76: 135-142.

Danovaro R., Gambi C., Mirto S., et al. 2004. Meiofauna. Biol. Mar. Mediterr. 11: 55-97.

Deprez T. et al. 2005. NeMys. World Wide Web electronic publication.

Fabbrocini A., Guarino A., Scirocco T., et al. 2005. Integrated biomonitoring assessment of the Lesina Lagoon (Southern Adriatic Coast, Italy): preliminary results. Chem. Ecol. 21: 479-489.

Fabbrocini A., Di Stasio M., D'Adamo R. 2010. Computerized sperm motility analysis in toxicity bioassays: A new approach to pore water quality assessment. Ecotoxicol. Environ. Saf. 73: 1588-1595. PMid:20537390

Ferrero T.J., Debenham N.J., Lambshead P.J.D. 2008. The nematodes of the Thames estuary: Assemblage structure and biodiversity, with a test of Attrill's linear model. Estuar. Coast. Shelf. Sci. 79: 409-418.

Forster S.J. 1998. Osmotic stress tolerance and osmoregulation of intertidal and subtidal nematodes. J. Exp. Mar. Biol. Ecol. 224: 109-125.

Frontalini F., Coccioni R. 2011. Benthic foraminifera as bioindicators of pollution: a review of Italian research over the last three decades. Rev. Micropal. 54: 115-127.

Frontalini F., Coccioni R., Bucci C. 2010. Benthic foraminiferal assemblages and trace element contents from the lagoons of Orbetello and Lesina. Environ. Monit. Assess. 170: 245-260. PMid:19911292

Frontalini F., Semprucci F., Coccioni R., et al. 2011. On the quantitative distribution and community structure of the meio and macrofaunal communities in the coastal area of the Central Adriatic Sea (Italy). Environ. Monit. Assess. 180: 325-344. PMid:21120690

Frontalini F., Margaritelli G., Francescangeli F., et al. 2013. Benthic foraminiferal assemblages and biotopes in a coastal lake: the case study of Lake Varano (southern Italy). Acta. Protozool. 52: 147-161.

Frontalini F., Semprucci F., Armynot du Châtelet E., et al. 2014. Biodiversity trends of the meiofauna and foraminifera assemblages of Lake Varano (southern Italy). Proc. Biol. Soc. Wash. 127.

Gambi C., Totti C., Manini E. 2003. Impact of organic loads and environmental gradients on microphytobenthos and meiofaunal distribution in a coastal lagoon. Chem. Ecol. 19: 207-223.

Gollner S., Riemer B., Martinez Arbizu P., et al. 2010. Diversity of Meiofauna from the East Pacific Rise across a Gradient of Hydrothermal Fluid Emissions. PLoS ONE 5: e12321. PMid:20856898 PMCid:PMC2938375

Graf G. 1992. Benthic-pelagic coupling: A benthic view. Oceanogr. Mar. Biol. Annu. Rev. 30: 149-190.

Guerrini A., Colangelo M.A., Ceccherelli V.U. 1998. Recolonization patterns of meiobenthic communities in brackish vegetated and unvegetated habitats after induced hypoxia/anoxia. Hydrobiologia 375/376: 73-87.

Guilini K., Levin L.A., Vanreusel A. 2012. Cold seep and oxygen minimum zone associated sources of margin heterogeneity affect benthic assemblages, diversity and nutrition at the Cascadian margin (NE Pacific Ocean). Prog. Oceanogr. 96: 77-92.

Heip C., Vincx M., Vranken G. 1985. The ecology of marine nematodes. Oceanogr. Mar. Biol. Annu. Rev. 23: 399-489.

Hendelberg M., Jensen P. 1993. Vertical distribution of the nematode fauna in coastal sediment influenced by seasonal hypoxia in the bottom water. Ophelia 37: 83-94.

Lambshead P.J.D., Tietjen J., Ferrero T.J., et al. 2000. Latitudinal diversity gradients in the deep sea with special reference to North Atlantic nematodes. Mar. Ecol. Prog. Ser. 194: 159-167.

Long E.R., MacDonald D.D., Smith S.L., et al. 1995. Incidence of adverse biological effects within ranges of chemical concentrations in marine and estuarine sediments. Environ. Manage. 19: 81-97.

Martins V.A, Frontalini F., Figueira R.C.L., et al. 2013. Assessment of the health quality of Ria de Aveiro (Portugal): heavy metals and benthic foraminifera. Mar. Pollut. Bull. 70: 18-33. PMid:23582314

Moreno M., Semprucci F., Vezzulli L., et al. 2011. The use of nematodes in assessing ecological quality status in the Mediterranean coastal ecosystems. Ecol. Ind. 11: 328-336.

Muthumbi A.W., Vanreusel A., Duineveld G., et al. 2004. Nematode community structure along the continental slope off the Kenyan Coast, Western Indian Ocean. Int. Rev. Hydrobiol. 89: 188-205.

Nicholas W.L., Goodchild D.J., Steward A. 1987. The mineral composition of intracellular inclusions in nematodes from thiobiotic mangrove mud-flats. Nematologica 33: 167-179.

Pallo P., Widbom B., Olafsson E. 1998. A quantitative survey of the benthic meiofauna in the Gulf of Riga (eastern Baltic Sea), with special reference to the structure of nematode assemblages. Ophelia 49: 117-139.

Pfannkuche O., Thiel H. 1988. Sample processing. In: Higgins R.P., Thiel H. (eds), Introduction to the study of meiofauna. Smithsonian Inst., Washington, pp. 134-145.

Pusceddu A., Gambi C., Manini E., et al. 2007. Trophic state, eco system efficiency and biodiversity of transitional aquatic ecosystems: analysis of environmental quality based on different benthic indicators. Chem. Ecol. 23: 505-515.

Schratzberger M., Warwick R.M. 1998. Effects of the intensity and frequency of organic enrichment on two estuarine nematode communities. Mar. Ecol. Prog. Ser. 164: 83-94.

Schratzberger M., Bolam S.G., Whomersley P., et al. 2003. Development of a meiobenthic nematode community following the intertidal placement of various types of sediment. J. Exp. Mar. Biol. Ecol. 303: 79-96.

Seinhorst J.W. 1959. A rapid method for the transfer of nematodes from fixative to anhydrous glycerine. Nematologica 4: 67-69.

Semprucci F. 2013. Marine nematodes from the shallow subtidal coast of the Adriatic Sea: species list and distribution. Int. J. Biodiv. 1: 1-9.

Semprucci F., Balsamo M. 2012. Key role of free-living nematodes in the marine ecosystem. In: Boeri F., Jordan A.C. (eds). Nematodes: Morphology, Functions and Management Strategies. NOVA Science Publishers, Inc. Hauppauge, New York, pp. 109-134.

Semprucci F., Colantoni P., Baldelli G., et al. 2010a. The distribution of meiofauna on back-reef sandy platforms in the Maldives (Indian Ocean). Mar. Ecol. 31: 592-607.

Semprucci F., Boi P., Manti A., et al. 2010b. Benthic communities along a littoral of the Central Adriatic Sea (Italy). Helgol. Mar. Res. 64: 101-115.

Semprucci F., Frontalini F., Covazzi-Harriague A., et al. 2013. Meio- and Macrofauna in the marine area of the Monte St. Bartolo Natural Park (Central Adriatic Sea, Italy). Sci. Mar. 77: 189-199.

Semprucci F., Colantoni P., Sbrocca C., et al. 2014. Spatial patterns of distribution of meiofaunal and nematode assemblages in the Huvadhoo lagoon (Maldives, Indian Ocean). J. Mar. Biol. Ass. UK.

Spagnoli F., Specchiulli A., Scirocco T., et al. 2002. The Lago di Varano: Hydrologic characteristics and sediment composition. Mar. Ecol. 23: 384-394.

Specchiulli A., Focardi S., Renzi M., et al. 2008. Environmental heterogeneity patterns and assessment of trophic levels in two Mediterranean lagoons: Orbetello and Varano, Italy. Sci. Tot. Environ. 402: 285-298. PMid:18550150

Specchiulli A., Renzi M., Scirocco T., et al. 2010. Comparative study based on sediment characteristics and macrobenthic communities in two Italian lagoons. Environ. Monit. Assess. 160: 237-256. PMid:19096908

Steyaert M., Vanaverbeke J., Vanreusel A., et al. 2003. The importance of fine-scale, vertical profiles in characterizing nematode community structure. Estuar. Coast. Shelf. Sci. 58: 353-366.

Steyaert M., Moodley L., Vanaverbeke J., et al. 2005. Laboratory experiments on the infaunal activity of intertidal nematodes. Hydrobiologia 540: 217-223.

Steyaert M., Moodley L., Nadong T., et al. 2007. Responses of intertidal nematodes to short-term anoxic events. J. Exp. Mar. Biol. Ecol. 345: 175-184.

Timm R.W. 1963. Marine nematodes of the suborder Monhysterina from the Arabian Sea at Karachi. Proc. Helminthol. Soc. Wash. 30: 34-49.

Travizi A., Vidakovic J. 1997. Nematofauna in the Adriatic Sea: Review and check-list of free-living nematode species. Helgol. Meeresunters. 51: 503-519.

Trentesaux A., Recourt P., Bout-Roumazeilles V., et al. 2001. Carbonate grain-size distribution in hemipelagic sediments from a laser particle sizer. J. Sediment. Res. 71: 858-862.

Vanaverbeke J., Gheskiere T., Steyaert M., et al. 2002. Nematode assemblages from subtidal sandbanks in the southern bight of the north sea: effect of small sedimentological differences. J. Sea. Res. 48: 197-207.

Vanaverbeke J., Merckx B., Degraer S., et al. 2011. Sediment-related distribution patterns of nematodes and macrofauna: Two sides of the benthic coin? Mar. Environ. Res. 71: 31-40. PMid:20974491

Villano N., Warwick R.M. 1995. Meiobenthic communities associated with the seasonal cycle of growth and decay of Ulva rigida Arardh in the Palude Della Rosa, Lagoon of Venice. Estuar. Coast. Shelf. Sci. 4: 181-194.

Warwick R.M. 1971. Nematode associations in the Exe Estuary. J. Mar. Biol. Assoc. U.K. 51: 430-454.

Warwick R.M., Gee J.M. 1984. Community structure of estuarine meiobenthos. Mar. Ecol. Prog. Ser. 18: 97-111.

Wetzel M.A., Weber A., Giere O. 2002. Re-colonization of anoxic/ sulfidic sediments by marine nematodes after experimental removal of macroalgal cover. Mar. Biol. 141: 679-689.

Wieser W. 1953. Die Beziehung zwischen Mundhöhlengestalt. Ernährungsweise und Vorkommen bei freilebenden marinen Nematoden. Eine okologisch-morphologische studie. Ark. Zool. 4: 439-484.

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