TY - JOUR AU - Sun, Shengming AU - Ge, Xianping AU - Zhu, Jian AU - Fu, Hongtuo AU - Jiang, Zhiqiang PY - 2014/03/30 Y2 - 2024/03/29 TI - Effects of water-borne copper on the survival, antioxidant status, metallothionein-I mRNA expression and physiological responses of the Chinese mitten crab, Eriocheir sinensis (Decapoda: Brachyura) larvae JF - Scientia Marina JA - Sci. mar. VL - 78 IS - 1 SE - Articles DO - 10.3989/scimar.03929.06B UR - https://scientiamarina.revistas.csic.es/index.php/scientiamarina/article/view/1497 SP - 91-97 AB - The lethal concentration of water-borne copper in Chinese mitten crab <em>Eriocheir sinensis</em> larvae was tested by exposing the animals to 0, 0.1, 0.2, 0.3, 0.5 and 0.8 mg Cu L<sup>-1</sup> at 20°C for 96 h. The 96-h median lethal concentration (LC50) and its corresponding 95% confident interval was estimated on zoea 1 larvae and megalopa larvae, respectively. Acute dissolved copper toxicity was higher for zoea 1 larvae (0.16 mg L<sup>-1</sup>) than for megalopa larvae (0.21 mg L<sup>-1</sup>). The antioxidant status, metallothionein-I mRNA expression and physiological response of the crab to copper toxicity was further investigated by exposing the megalopa larvae to 0, 0.08 and 0.16 mg Cu L<sup>-1</sup> for 96 h. The superoxide dismutase activity, catalase activity, glutathione S-transferase (GST) activity and lipid peroxidation content of megalopa larvae increased concomitantly with the exposure time and copper concentration. MT-I mRNA expression levels were positively correlated with both the concentration and duration of copper exposure. The oxygen consumption and respiratory quotient of megalopa larvae in response to 0.16 mg L<sup>-1</sup> copper were significantly higher than those in the control group after 96 h of exposure (P < 0.05). The results of this study highlight the potential effects of copper as a common stressor in <em>E. sinensis</em> larvae. MT-I and GST appear to be suitable biomarkers of environmental copper exposure stress in <em>E. sinensis</em> larvae ER -