@article{Quijano-Scheggia_Garcés_Andree_de la Iglesia_Diogène_Fortuño_Camp_2010, title={Pseudo-nitzschia species on the Catalan coast: characterization and contribution to the current knowledge of the distribution of this genus in the Mediterranean Sea}, volume={74}, url={https://scientiamarina.revistas.csic.es/index.php/scientiamarina/article/view/1169}, DOI={10.3989/scimar.2010.74n2395}, abstractNote={Proliferations of the genus <i>Pseudo-nitzschia</i> recur along the Catalan coast (NW Mediterranean) throughout the year. The establishment of 58 clonal cultures facilitated morphological studies with scanning electron microscopy (SEM) and ITS 5.8S rDNA sequence characterization. Moreover, strains of each species were examined with respect to sexual compatibility and toxicity. The results of the morphological and phylogenetic studies confirmed nine species of the genus <i>Pseudo-nitzschia: P. brasiliana, P. calliantha, P. delicatissima</i> clade A/del 2, <i>P. arenysensis, P. fraudulenta, P. galaxiae, P. linea, P. multistriata</i> and <i>P. pungens</i> clade I. Moreover, two <i>Pseudo-nitzschia</i> species, <i>P. caciantha</i> and <i>P. cf mannii</i>, could only be identified following SEM analysis of their morphology. None of the cultured strains of Pseudo-nitzschia analyzed produced domoic acid in amounts above the limit of detection. The current distributions of the <i>Pseudo-nitzschia</i> species in the Mediterranean Sea were plotted on maps, which led to the following observations: i) diversity within this genus is much greater than previously considered, ii) some species have a broad distribution (e.g. <i>P. calliantha</i>), iii) whereas the distribution of others is narrowly restricted (e.g. <i>P. pungens</i> clade I). Moreover, this study reports the first detection of <i>P. linea</i> in the Mediterranean Sea and is the first description of <i>P. galaxiae</i> and <i>P. cf mannii</i> along the Catalan coast. Morphological studies coupled with molecular biological characterization, mating tests and biogeographic distribution analyses provide a critical theoretical basis for testing and/or implementing the current phylogenetic framework in the genus <i>Pseudo-nitzschia</i>.}, number={2}, journal={Scientia Marina}, author={Quijano-Scheggia, Sonia and Garcés, Esther and Andree, Karl B. and de la Iglesia, Pablo and Diogène, Jorge and Fortuño, José Manuel and Camp, Jordi}, year={2010}, month={Jun.}, pages={395–410} }