Effects of light quality on reproduction, growth and pigment content of Gracilaria birdiae (Rhodophyta: Gracilariales)


  • José Bonomi Barufi Departamento de Botânica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina
  • Félix L. Figueroa Departamento de Ecología y Geología, Facultad de Ciencias, Universidad de Málaga
  • Estela M. Plastino Departamento de Botânica, Instituto de Biociências da Universidade de São Paulo




Gracilaria birdiae, growth, light quality, pigment content, photoacclimation, reproduction


Gracilaria species are the main source of agar worldwide. Since laboratory cultivation is an important means of sustaining such production, this study aimed to assess the influence of two fundamental strategies of physiological algae adjustment, photoacclimation and photomorphogenesis, on G. birdiae physiology. Specifically, the effects of different spectral light qualities on reproduction, growth, and pigment content in two distinct photoperiods (8 and 14 hours) were examined. Tetrasporophytes and gametophytes were submitted to different light qualities: white, green, red, and blue over the course of nine weeks. At the end of this period, chlorophyll a, allophycocyanin, phycocyanin, and phycoerythrin contents were analysed. Gametophytes showed reproductive structures only under monochromatic radiation. A stimulatory effect on tetrasporangium differentiation was mainly observed under red light, but this high fertility was negatively correlated with growth rate, i.e. algae cultivated under red light showed the lowest growth rate. However, while growth rates were higher in algae exposed to white light, they were similar to those observed in algae subjected to green light and 14 hours of daylight. PE was the predominant pigment, irrespective of light quality. Phycocyanin and phycoerythrin concentration increased when algae were cultivated under green and blue light. Therefore, considering future management prospects for G. birdiae mariculture, we suggest that red light could be utilized as a reproductive inductor to produce tetraspores. Furthermore, if the aim is to achieve high phycoerythrin content, continuous blue light could be applied during a short photoperiod. Indeed, the combination of different wavelengths could allow better economic resource exploitation.


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Armisén R. 1995. World-wide use and importance of Gracilaria. J. Appl. Phycol. 7: 231-243. http://dx.doi.org/10.1007/BF00003998

Ayres-Ostrock L.M., Plastino E.M. 2014a. Effects of short-term exposure to ultraviolet-B radiation on photosynthesis and pigment content of red (wild types), greenish-brown, and green strains of Gracilaria birdiae (Gracilariales, Rhodophyta). J. Appl. Phycol. 26: 867-879. http://dx.doi.org/10.1007/s10811-013-0131-3

Ayres-Ostrock L.M., Plastino E.M. 2014b. Effects of UV-B radiation on growth rates, pigment content and ultrastructure of red (wild type), greenish-brown and green strains of Gracilaria birdiae (Gracilariales, Rhodophyta). Eur. J. Phycol. 49: 197-212. http://dx.doi.org/10.1080/09670262.2014.904931

Barufi J.B., Korbee N., Oliveira M.C., et al. 2011. Effects of N supply on the accumulation of photosynthetic pigments and photoprotectors in Gracilaria tenuistipitata (Rhodophyta) cultured under UV radiation. J. Appl. Phycol. 23: 457-466. http://dx.doi.org/10.1007/s10811-010-9603-x

Beer S., Levy I. 1983. Effects of photon fluence rate and light spectrum composition on growth, photosynthesis and pigment relations in Gracilaria sp. J. Phycol. 19: 516-522. http://dx.doi.org/10.1111/j.0022-3646.1983.00516.x

Berner T., Dubinsky Z., Wyman K., et al. 1989. Photoadaptation and the "package" effect in Dunaliella tertiolecta (Chlorophyceae). J. Phycol. 25: 70-78. http://dx.doi.org/10.1111/j.0022-3646.1989.00070.x

Carneiro M.A.A., Marinho-Soriano E., Plastino E.M. 2011. Phenology of an agarophyte Gracilaria birdiae Plastino and E.C. Oliveira (Gracilariales, Rhodophyta) in Northeastern Brazil. Rev. Bras. Farmacogn. 21: 317-322. http://dx.doi.org/10.1590/S0102-695X2011005000067

Costa V.L., Plastino E.M. 2001. Histórico de vida de espécimens selvagens e variantes cromáticas de Gracilaria sp. (Gracilariales, Rhodophyta). Rev. Brasil. Bot. 24: 491-500.

Costa V.L., Plastino E.M. 2011. Color inheritance and pigment characterization of red (wild-type), greenish-brown, and green strains of Gracilaria birdiae (Gracilariales, Rhodophyta). J. Appl. Phycol. 23: 599-605. http://dx.doi.org/10.1007/s10811-010-9642-3

Costa E.S., Plastino E.M., Petti R., et al. 2012. The Gracilariaceae germoplasm bank of the University of São Paulo, Brazil a DNA barcoding approach. J. Appl. Phycol. 1: 1-11.

Demmig-Adams B. 2003. Linking the xanthophyll cycle with thermal energy dissipation. Photosynt. Res. 76: 73-80. http://dx.doi.org/10.1023/A:1024902927403 PMid:16228567

Dring M.J. 1988. Photocontrol of development in algae. Ann. Rev. Plant. Physiol. Plant. Mol. Biol. 39: 157-174. http://dx.doi.org/10.1146/annurev.pp.39.060188.001105

Dring M.J., Lüning K. 1973. Induction of two-dimensional growth and hair formation by blue light in the brown alga Scytosiphon lomentaria. Z. Pflanzenphysiol. Bd. 75: 107-117. http://dx.doi.org/10.1016/S0044-328X(75)80107-3

Dring M.J., Lüning K. 1975. A photoperiodic response mediated by blue light in the brown algae Scytosiphon lomentaria. Planta (Berl.) 125: 25-32. http://dx.doi.org/10.1007/BF00388870 PMid:24435297

Falkowski P.G., LaRoche J. 1991. Acclimation to spectral irradi- ance in algae. J. Phycol. 27: 8-14. http://dx.doi.org/10.1111/j.0022-3646.1991.00008.x

Figueroa F.L., Aguilera J., Niell F.X. 1995a. Red and blue light regulation of growth and photosynthetic metabolism in Por- phyra umbilicalis (L.) Kützing (Bangiales, Rhodophyta). Eur. J. Phycol. 30: 11-18. http://dx.doi.org/10.1080/09670269500650761

Figueroa F.L., Aguilera J., Jiménez C., et al. 1995b. Growth, pig- ment synthesis and nitrogen assimilation in the red alga Por- phyra umbilicalis (L.) Kützing (Bangiales, Rhodophyta) under blue and red light. Sci. Mar. 59: 9-20.

Forster R.M., Dring M.J. 1994. Influence of blue light on the photosynthetic capacity of marine plants from different taxonomic, ecological and morphological groups. Eur. J. Phycol. 29: 21-27. http://dx.doi.org/10.1080/09670269400650441

Franklin L.A., Kräbs G., Kuhlenkamp R. 2001. Blue light and UV-A radiation control the synthesis of mycosporine-like amino acids in Chondrus crispus (Florideophyceae). J. Phycol. 37: 257-270. http://dx.doi.org/10.1046/j.1529-8817.2001.037002257.x

Gantt E. 1990. Pigmentation and photoacclimation. In: Cole K.M., Sheat R.G. (eds), Biology of the red algae. Cambridge University Press, Cambridge, pp 203-219.

Gerard V.A. 1997. Environmental stress during early development of kelp sporophytes (Laminaria saccharina): how long do effects persist? J. Appl. Phycol. 9: 5-9. http://dx.doi.org/10.1023/A:1007952030505

Godínez-Ortega J.L., Snoeijs P., Robledo D., et al. 2008. Growth and pigment composition in the red alga Halymenia floresii cultured under different light qualities. J. Appl. Phycol. 20: 253-260. http://dx.doi.org/10.1007/s10811-007-9241-0

Grzymski J., Johnsen G., Sakshug E. 1997. The significance of intracellular self-shading on the bio-optical properties of brown, red and green macroalgae. J. Phycol. 33: 408-414. http://dx.doi.org/10.1111/j.0022-3646.1997.00408.x

Guimarães M., Plastino E.M., Oliveira E.C. 1999. Life history, reproduction and growth of Gracilaria domingensis (Gracilariales, Rhodophyta) from Brazil. Bot. Mar. 42: 481-486. http://dx.doi.org/10.1515/BOT.1999.055

Jeffrey W., Humphrey G.F. 1975. New spectrophotometric equations for determining chlorophylls a, b, c1 and c2 in higher plants, algae and natural phytoplankton. Biochem. Physiol. Pflanzen. (BPP) Bd. 167: 191-194.

Kursar T.A., Van Der Meer J., Alberte R.S. 1983a. Light-harvesting system of the red alga Gracilaria tikvahiae. I. Biochemical analyses of pigment mutations. Plant Physiol. 73: 353-360. http://dx.doi.org/10.1104/pp.73.2.353 PMid:16663220 PMCid:PMC1066465

Kursar T.A., Van Der Meer J., Alberte R.S. 1983b. Light-harvesting system of the red alga Gracilaria tikvahiae. II. Phycobilissome characteristics of pigment mutants. Plant Physiol. 73: 361-369. http://dx.doi.org/10.1104/pp.73.2.361 PMid:16663221 PMCid:PMC1066466

Lapointe B.E., Dawes C.J., Tenore K.R. 1984. Interactions between light and temperature on the physiological ecology of Gracilaria tikvahiae (Gracilariales, Rhodophyta). II. Nitrate uptake and levels of pigments and chemical constituents. Mar. Biol. 80: 171-178. http://dx.doi.org/10.1007/BF02180184

Lignell A., Pedersén M. 1989. Agar composition as a function of morphology and growth rate. Studies on some morphological strains of Gracilaria secundata and Gracilaria verrucosa (Rhodophyta). Bot. Mar. 32: 219-227. http://dx.doi.org/10.1515/botm.1989.32.3.219

López-Figueroa F. 1991. Red, blue and green photoreceptors controlling chlorophyll, biliprotein and total protein synthesis in the red alga Chondrus crispus. Br. Phycol. J. 26: 383-392. http://dx.doi.org/10.1080/00071619100650351

López-Figueroa F., Niell F.X. 1989. Red-light and blue-light photoreceptors controlling chlorophyll a synthesis in the red alga Porphyra umbilicalis and in the green alga Ulva rigida. Physiol. Plantarum. 76: 391-397. http://dx.doi.org/10.1111/j.1399-3054.1989.tb06209.x

López-Figueroa F., Niell F.X. 1991. Photocontrol of chlorophyll and biliprotein synthesis in seaweeds: possible photoreceptors and ecological considerations. Sci. Mar. 55: 519-527.

López-Figueroa F., Lindemann P., Braslavsky S.E., et al. 1989. Detection of a phytochrome -like protein in macroalgae. Bot. Acta. 102: 178-180. http://dx.doi.org/10.1111/j.1438-8677.1989.tb00089.x

Lourenço S.O., Vieira A.A.H. 2004. Culture collections of microalgae in Brazil: progress and constraints. Nova Hedwigia 79: 149-173. http://dx.doi.org/10.1127/0029-5035/2004/0079-0149

Lüning K. 1992. Day and night kinetics of growth rate in green, brown and red seaweeds. J. Phycol. 28: 794-803. http://dx.doi.org/10.1111/j.0022-3646.1992.00794.x

Lüning K., Dring, M.J. 1973. The influence of light quality on the development of the brown algae Petalonia and Scytosiphon. Br. Phycol. J. 8: 333-338. http://dx.doi.org/10.1080/00071617300650361

Lüning K., Dring M.J. 1985. Action spectra and spectral quantum yield of photosynthesis in marine macroalgae with thin and thick thalli. Mar. Biol. 87: 119-129. http://dx.doi.org/10.1007/BF00539419

Marquardt R., Schubert H., Varela D.A., et al. 2010. Light acclimation strategies of three commercially important red algal species. Aquaculture 299: 140-148. http://dx.doi.org/10.1016/j.aquaculture.2009.11.004

Müller S., Clauss H. 1976. Aspects of photomorphogenesis in the brown alga Dictyota dichotoma. Zeitschrift. fur Pflanzen. 78: 461-465. http://dx.doi.org/10.1016/S0044-328X(76)80095-5

Monro K., Poore A.G.B. 2005. Light quantity and quality induce shade-avoiding plasticity in marine macroalgae. J. Evol. Biol. 18: 426-435. http://dx.doi.org/10.1111/j.1420-9101.2004.00826.x PMid:15715848

Ohki K., Fujita Y. 1992. Photoregulation of phycobilisome structure during complementary chromatic adaptation in the marine cyanophyte Phormidium sp. C86. J. Phycol. 28: 803-808. http://dx.doi.org/10.1111/j.0022-3646.1992.00803.x

Plastino E.M., Guimarães M. 2001. Diversidad intraespecífica. In: Alveal K., Antezana T. (eds) Sustentabilidad de la biodiversidad. Universidad de Concepción, Concepción, pp. 19-27.

Plastino E.M., Oliveira E.C. 1990. Crossing experiments as an aid to the taxonomic recognition of the agarophyte alga Gracilaria (Rhodophyta, Gigartinales). In: Oliveira E.C., Kautsky N. (eds) Cultivation of Seaweeds in Latin American. Universidade de São Paulo, São Paulo, pp. 127-133.

Plastino E.M., Oliveira E.C. 2002. Gracilaria birdiae (Gracilariales, Rhodophyta), a new species from the tropical south American Atlantic with a terete frond and deep spermatangial conceptacles. Phycologia 41: 389-396. http://dx.doi.org/10.2216/i0031-8884-41-4-389.1

Plastino E.M., Ursi S., Fujii M.T. 2004. Color inheritance, pigment characterization, and growth of a rare light green strain of Gracilaria birdiae (Gracilariales, Rhodophyta). Phycol. Res. 52: 45-52. http://dx.doi.org/10.1111/j.1440-1835.2004.tb00314.x

Rüdiger W., López-Figueroa F. 1992. Photoreceptors in algae. Ann. Rev. Photochem. Photobiol. 55: 949-954. http://dx.doi.org/10.1111/j.1751-1097.1992.tb08542.x

Sagert S., Schubert H. 1995. Acclimation of the photosynthetic apparatus of Palmaria palmata (Rhodophyta) to light qualities that preferentially excite photosystem I or photosystem II. J. Phycol. 31: 547-554. http://dx.doi.org/10.1111/j.1529-8817.1995.tb02548.x

Santelices B., Varela D. 1995. Regenerative capacity of Gracilaria fragments: effects of size, reproductive state and position along the axis. J. Appl. Phycol. 7: 501-506. http://dx.doi.org/10.1007/BF00003935

Talarico L., Maranzana G. 2000. Light and adaptive responses in red macroalgae: an overview. J. Photochem. Photobiol. B. Biol. 56: 1-11. http://dx.doi.org/10.1016/S1011-1344(00)00046-4

Thirb H.H., Benson-Evans K. 1983. The effect of different light intensities and wavelengths on carpospore germination and the apical tips of the red alga Lemanea Bory (1808). Nova Hedwigia 37: 669-682.

Tsekos I., Niell F.X., Aguilera J., et al. 2002. Ultrastructure of the thallus stage of Porphyra leucosticta (Rhodophyta) grown in red, blue and green light. Phycol. Res. 50: 251-264. http://dx.doi.org/10.1111/j.1440-1835.2002.tb00158.x

Ursi S., Plastino E.M. 2001. Crescimento in vitro de linhagens de coloração vermelha e verde clara de Gracilaria birdiae (Gracilariales, Rhodophyta) em dois meios de cultura: análise de diferentes estádios reprodutivos. Rev. Brasil. Bot. 24: 585-592. http://dx.doi.org/10.1590/s0100-84042001000500014

Ursi S., Pedersén M., Plastino E.M., et al. 2003. Intraspecific variation of photosynthesis, respiration and photoprotective carotenoids in Gracilaria birdiae (Gracilariales, Rhodophyta). Mar. Biol. 142: 997-1007.

Ursi S., Guimarães M., Plastino E.M. 2008. Deleterious effects of TRIS buffer on growth rates and pigment content of Gracilaria birdiae Plastino and E.C. Oliveira (Gracilariales, Rhodophyta). Acta. Bot. Bras. 22: 891-896. http://dx.doi.org/10.1590/S0102-33062008000300024

Zar J.H. 1999. Biostatistical analysis. Prentice Hall, Upper Saddle River, New Jersey.



How to Cite

Bonomi Barufi J, Figueroa FL, Plastino EM. Effects of light quality on reproduction, growth and pigment content of Gracilaria birdiae (Rhodophyta: Gracilariales). scimar [Internet]. 2015Mar.30 [cited 2023Feb.7];79(1):15-24. Available from: https://scientiamarina.revistas.csic.es/index.php/scientiamarina/article/view/1571