The genus Paguristes ( Crustacea : Decapoda : Diogenidae ) from Cuba ( Western Atlantic ) . A new record and a new species *

The genus Paguristes Dana, 1852, is the largest genus of the family Diogenidae distributed in shallow waters of all tropical and subtropical areas and is well represented in the West Indian region. The study of this genus started with Dana (1852) describing the species collected in the United States Exploring Expedition, from 1838 to 1842. At the end of 19th century there were some authors who worked on this genus, such as Stimpson (1859), A. Milne Edwards (1880), A. Milne Edwards and Bouvier (1893), Benedict (1901), Bouvier (1918) and Schmitt (1933, 1935). Since the 1950s, these studies have not been stopped and many species have been described by Forest (1954), Wass (1955, 1963), Holthuis (1959), Provenzano (1959, 1962, 1965), Forest and Saint Laurent (1967), MacLaughlin and Provenzano (1974 a,b), Martínez-Iglesias and Gómez-Hernández (1989), Campos and Sánchez (1995), and Sandberg (1996). MacLaughlin and Provenzano (1974 a) reported on seven species of the Paguristes tortugae Schmitt, 1933 complex, four of which were described as new species. That paper constituted the first part of a continuing series of reports on regional Paguristes species. At that time, the authors knew of the existence of at least three additional species complexes. In the second part of the series (MacLaughlin and Provenzano, 1974 b), they described six new species of Paguristes, but none of these were associated with the three previously recognized complexes. Within this genus there are a number of problematic species, probably because morphological SCI. MAR., 66 (2): 135-143 SCIENTIA MARINA 2002

MacLaughlin and Provenzano (1974 a) reported on seven species of the Paguristes tortugae Schmitt, 1933 complex, four of which were described as new species.That paper constituted the first part of a continuing series of reports on regional Paguristes species.At that time, the authors knew of the existence of at least three additional species complexes.In the second part of the series (MacLaughlin and Provenzano, 1974 b), they described six new species of Paguristes, but none of these were associated with the three previously recognized complexes.
The aim of the present study is to increase the knowledge of the genus Paguristes in the Caribbean Sea and specifically on the Cuban coast.

MATERIAL AND METHODS
The material studied was collected during different surveys of the "Instituto de Oceanología de Cuba" in 1987 and1988 in shallow waters (2-10 m depth), by a benthic sucker air machine and by hand.The sampling areas, located in the North and South of Cuba, were: Medano la Vela in the North Keys of Sabana-Camagüey Islands (Fig. 1A); Vita Bay and Jururú Bay in Província de Holguin, N.E.Cuba (Fig. 1C), and in the Juan García Reef, Cantiles Reef and Diego Pérez Reef in Batabanó Gulf (Fig. 1B).

Paguristes paraguanensis
Material examined: Juán García Reef, Batábano Gulf, SW Cuba, 1-3-1988: (126-14) 1 (. Remarks: P. paraguanensis is recorded for the first time in Cuban waters.This species could be confused with P. erythrops; both species are present in Cuba and they are sympatric in the coral reefs in Batabanó Gulf.The main character which can help to distinguish P. paraguanensis and P. erythrops from most other species of Paguristes is the presence of spines on the fifth segments of the antennal peduncles (MacLaughlin and Provenzano, 1974b).On the other hand, the most reliable morphological characters to differentiate these species from each other are: (1) the presence of a prominent spine at the dorsodistal margin of the carpus of the fourth pereiopod, which is present in P. erythrops and absent in P. paraguanensis (McLaughlin and THE GENUS PAGURISTES FROM CUBA 137  Williams (1984), based on Provenzano and Rice (1966).On the other hand, Paguristes taeniatus mentioned in Benedict (1901) is a Clibanarius (R. Lemaitre, pers.comm.)].
Caribbean Species Cuban Species P. anaryballus Provenzano, 1965.Provenzano, 1974 b) and ( 2) the coloration: a redorange coloration in the ocular peduncles and an orange-spotted appearance in the chelipeds and ambulatory legs could be observed in well preserved specimens of P. paraguanensis, whereas P. erythrops (prone to lose the color before than P. paraguanensis) has carmine ocular peduncles and prominent reddish patches on the dorsal surfaces of the palms and carpus of the chelipeds and on the mesial and lateral faces of the propodus, carpus and merus of the ambulatory legs.
Paguristes holguinensis sp.nov.Etymology: This species is named after the type locality, Holguin.
Description: Shield (Fig. 2A) longer than broad; dorsal surface rugose, with some small spines marginally and laterally, mainly in the anterolateral parts of shield, and sparse short setae.Lateral projections broadly triangular, acute with terminal spinule.Rostrum obtuse, not reaching the level of lateral projections.
Ocular peduncles (Fig. 2C) slender, much longer than half the length of shield, with long setae on the proximal half of dorsomesial margin, mainly proximally.Ocular acicles large, separated, with strong bifid terminal spines (in one specimen, 2VA-15, they are not bifid), with the lateral and mesial faces of acicles straight and without spines (only in one acicle of one specimen was a small spine found in the middle of the mesial face) and the basal parts are not broadly dilated.
Antennular peduncles (Fig. 2B) when fully extended overreach the distal margins of corneas by approximately 1/5 to nearly the entire length of the ultimate segment.Ultimate and penultimate segments without setae.Basal segment ending in two lobes, each one with a spine in the distal mar-gin, and another in the dorsolateral margin of the statocyst lobe.
Antennal peduncles (Fig. 2C) overreaching the midlength of the ocular peduncle.Fifth segment nude, projected ventrally.Third segment with a strong distal spine.Second segment with two strong spines in the dorsal margin, one mesial and one lateral.Antennal acicle overreaching the half length of the fifth segment, with two or three spines in the dorsolateral and two or three spines in the dorsomesial margins and a terminal one; ventral side nude.Antennal flagellum short, in most cases equal to or longer than carapace; each article with short (one article in length) and long (length variable: two and a half article in length, in most specimens) setae in the distal margin.Mandible (Fig. 3A) with a palp with three segments; first segment smaller than second, and second smaller than the third.Maxillule (Fig. 3B) with internal endite subquadrate; endopodite with several (4-5) setae on the internal lobe, external lobe well developed, curved, more than half the endopodal length.Maxilla (Fig. 3C) with endopodite slightly exceeding scaphognathite in distal extension.
Third maxilliped (Fig. 3F) with basis-ischium distinctive, with a visible suture, although basis is almost broken in the middle; basis with 4 small spines, 3 proximal and one distal; ischium with strong crista dentata well developed with a single row of 12 teeth and with a strong spine in the dor-  sodistal margin; no accessory tooth; merus with 2 spines on the distal-medial half of the ventral margin and with a strong spine in the dorsodistal margin; carpus, propodus and dactyl nude.Chelipeds (Fig. 4A, B, setae not shown) subequal; left slightly larger; armature generally similar.Dactyl slightly longer than palm, cutting edge with a row of calcareous teeth proximally, terminating in a corneous claw, dorsal surface with a row of small spinulose tubercles decreasing in size distally and scattered tufts of setae, outer face with tuft of setae and inner face nude.Palm longer than carpus; outer face convex with a dorsal row of 4 or 5 strong spines, with stiff setae, and with a row of 3 or 4 strong spinulose tubercles on the medial part and other smaller ones on the distal part, inner face with 2 ventrodistal tubercles, fixed finger with tuft of setae and some small tubercles (in general, each tubercle is usually accompanied by a tuft of stiff setae).Carpus almost as large as merus; outer face with 2 rows of strong spinulose tubercles: one dorsal with 5 or 6 spines and the other on the medial part with 4 spines, separated by one unarmed longitudinal strip.Merus with one dorsodistal spine and with a row of small spines (variable in number) in the dorsal margin, decreasing in size proximally, accompanied by tufts of moderately long setae; ventromesial and ventrolateral margins each one with a row of small tubercles.
Second pereiopod (Figs.4C, D) moderately long; dactyl a little longer than the propodus of the cheliped (including the fixed finger) and 1.38 times longer than its propodus, moderately curved and terminating in a corneous-tipped spine; carpus 0.63 times the propodus, with 2 very small dorsal spinules (distally and proximally); merus 0.88 times the propodus length and with very small dorsal spinules (3-4).Third pereiopod (Figs.4E, F) very similar to the second one; only the dorsal spines on merus and especially the ones of the carpus are more developed (5-6 strong spines in the latter).Ventral surface of the segments of the second and third legs smooth, except in the ventrolateral crest of merus in which some small spines can be found, mainly in the third leg (where sometimes, some tubercles can be observed on the outer face).Fourth (Figs. 4G, H) and fifth (Figs.4I, J) pereiopods without any special distinctive character.
First and second pleopods of males are paired (Figs.5A-D) and modified as gonopods.First pleopods (Figs.5A, B) with basal segment with tuft of long stiff bristles at superior mesial angle; mesial margin with row of long fine setae; distal margin of inferior lamella with row of subacute holed spines; external lobe moderately slender slightly overreaching the inferior lamella; internal lobe shorter than external lobe in distal extension and set apart by prominent space, mesial and distal margins and distal third of inferior margin with long bristles and stiff setae.Second pleopods (Fig. 5C) with penultimate segment nude; ultimate segment with relatively long setae and appendix masculina with marginal bands of moderately short bristles.Third to fifth pleopods unpaired, with well developed exopodites.Female gonopores paired.First pleopods of females paired and modified (Fig. 5D), with basal segment as long as the distal one.Second to fifth pleopods unpaired (only on the left side), second to fourth with both rami well developed with long plumose setae in the whole pleopod, fifth with endopodite rudimentary.There is no brood pouch.Mean diameter of the eggs: 27.8 mm; standard deviation: ± 1.48 mm.
Telson (Fig. 5E) with practically symmetrical lobes, the left one very slightly larger than the right one, separated by a narrow median cleft delimited by two (sometimes three or four) spines in both margins.Terminal margins of left lobe with five to eight spines of small-medium size, the lateral are stronger; right lobe with a similar structure.
Coloration: Ocular peduncle iridescent (the proximal half of the peduncles show a dorsal white band in alcohol-preserved specimens).The antennular peduncles show a longitudinal red-iridescent band in the last article.Shield with red-iridescent patches.
Distribution: The specimens have only been found in the superficial coral reef between 2 and 3 m, in Vita Bay and Jururú Bay, province of Hologuín, NE of Cuba (Fig. 1).
Affinities: P. holguinensis is very easy to differentiate from the other species of Paguristes of the Caribbean Sea, with obtuse rostrum not reaching the level of the lateral projections, because of its separated ocular acicles having a strong bifid terminal spine.Only Paguristes planatus A. Milne Edwards and Bouvier, 1893, among the Caribbean species, presents both these characters.The most reliable characters for separating them are: a) the antennular peduncle length, which is longer in P. holguinensis than in P. planatus (in this species it only reaches the distal part of the cornea); b) the antennal acicle, which is more spinous in P. planatus; c) the absence of brood pouch in the females of P. holguinensis which carry the eggs directly on their pleopods and d) the thoracic sternum, which is very broad in P. planatus.

TABLE 1 .
-Check list of the Caribbean Sea species.In the right column the first references for Cuban waters are indicated.The species observed in this study are marked with (+).[Notes:Paguristes rectifromsBenedict, 1901 and Paguristes teunirostris Benedict, 1901, have been considered synonyms of Paguristes sericeus according to