Nutrient preservation, analysis precision and quality control of an oceanographic database of inorganic nutrients, dissolved oxygen and chlorophyll a from the NW Mediterranean Sea

Authors

  • Mariona Segura-Noguera Department of Marine Biology and Oceanography, Institut de Ciències del Mar, CSIC - School of Ocean and Earth Science and Technology, Center for Microbial Oceanography: Research and Education, University of Hawaii
  • Antonio Cruzado Department of Aquatic Biogeochemistry, Centre d’Estudis Avançats de Blanes, CSIC
  • Dolors Blasco Department of Marine Biology and Oceanography, Institut de Ciències del Mar, CSIC

DOI:

https://doi.org/10.3989/scimar.2011.75n2321

Keywords:

dissolved inorganic nutrients, dissolved oxygen, chlorophyll a, nutrient preservation, analysis precision, quality control, NW Mediterranean Sea

Abstract


Oceanographic data from 28 cruises performed in the NW Mediterranean Sea between 1982 and 2003 and data from historical databases, amounting to more than 100000 data points, are used to define the quality control ranges at standard level depths of dissolved inorganic nutrients, dissolved oxygen and chlorophyll a. The quality of each of the key variables and the analytical precision are assessed. The results indicate that freezing is a valid preservation method for phosphate, nitrate, silicate and nitrite in samples from the NW Mediterranean Sea, though their relative error increases (6.3%, –1.6%, –2.5% and –11.4%, respectively). In contrast, freezing nutrient samples on the cruises gathered negatively affected the analytical precision and accuracy of the ammonium analysis. The analysis precision is also estimated using different approximations, the most realistic being the use of replicate samples from the same sampling bottle. Except for phosphate and dissolved oxygen, specific quality control ranges for mixed water columns, usually found in winter, have to be defined, since the surface concentrations are particularly high due to deep water mixing. The quality-controls described in this work are an important and useful tool for validating data and for detecting erroneous or anomalous data obtained in both historical and future works in the NW Mediterranean Sea.

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References

Aminot, A. and R. Kérouel. – 1998. Pasteurization as an alternative method for preservation of nitrate and nitrite in seawater samples. Mar. Chem., 61: 203-208. doi:10.1016/S0304-4203(98)00021-8

Aminot, A. and D. Kirkwood. – 1995. Report on the results of the fifth ICES comparison exercise for nutrients in sea water. ICES Cooperative Research Report, 213. Copenhagen.

Aminot, A., R. Kérouel and S.C. Coverly. – 2009. Nutrients in Seawater Using Segmented Flow Analysis. In: O. Wurl (ed.), Practical Gudelines for the Analysis of Seawater. CRC Press, 143-178.

Avanzino, R.J. and V.C. Kennedy. – 1993. Long-term frozen storage of stream water samples for dissolved orthophosphate, nitrate plus nitrite, and ammonium analysis. Water Resour. Res., 29: 3357-3362. doi:10.1029/93WR01684

Bendschneider, K. and R. Robinson. – 1952. A new spectrophotometric method for the determination of nitrite in sea water. J. Mar. Res., 11(1): 87-96.

Benesch, R. and P. Mangelsdorf. – 1972. A method for the colorimetric determination of ammonium in sea water. Helgoländer Wiss Meeresunters, 23: 265-375.

Béthoux, J.P. and G. Copin-Montégut. – 1986.Biological fixation of atmospheric nitrogen in the Mediterranean Sea. Limnol. Oceanogr., 31: 1353-1358. doi:10.4319/lo.1986.31.6.1353

Béthoux, J.P., P. Morin, C. Chaumery, O. Connan, B. Gentili and D. Ruiz-Pino. – 1998. Nutrients in the Mediterranean Sea, mass balance and statistical analysis of concentrations with respect to environmental change. Mar. Chem., 63: 155-169. doi:10.1016/S0304-4203(98)00059-0

Béthoux, J.P., X. Durrieu de Madron, F. Nyffeler and D. Tailliez. – 2002. Deep water in the western Mediterranean. Peculiar 1999 and 2000 characteristics, shelf formation hypothesis, variability since 1970 and geochemical inferences. J. Mar. Sys., 33-34: 117-131. doi:10.1016/S0924-7963(02)00055-6

Bower, C.E. and T. Holm-Hansen. – 1980. A salicylate-hypochlorite method for determining ammonium in seawater. Can. J. Fish. Aquat. Sci., 37:794-798. doi:10.1139/f80-106

Carpenter, E.J. and J.J. McCarthy. – 1975. Nitrogen fixation and uptake of combined nitrogenous nutrients by Oscillatoria (Trichodesmium) thiebautii in western Sargasso Sea. Limnol. Oceanogr., 20: 389-401. doi:10.4319/lo.1975.20.3.0389

Chapman, P. And S.A. Mostert. – 1990. Does freezing of nutrient samples cause analytical errors. South African J. Mar. Sci., 9: 239-247. doi:10.2989/025776190784378763

Conkright, M.E., T.P. Boyer, S. Levitus. – 1994. Quality control and processing of historical oceanographic nutrient data. NOAA Technical Report NESDIS 79. NOAA, Washington DC.

Conkright, M.E., H.E. Garcia, T.D. O’Brien, R.A. Locarnini, T.P. Boyer and C. Stephens, J.I. Antonov. – 2000. World ocean atlas. Volume 4: Nutrients. NOAA Atlas NESDIS 52. NOAA, Washington DC.

Cruzado, A. – 1989. Automatic Techniques for Seawater Analysis. Info. Tec. CEAB Ocean., 23.

Culbertson, C.H., G. Knapp, M.C. Stalcup, R.T. Williams and F. Zemlyak. – 1991. A comparison of methods for the determination of dissolved oxygen in seawater. WHP Office Report WHPO-91-2.

DeGobbis, D. – 1973. On the storage of seawater samples for ammonium determination. Limnol. Oceanogr., 18: 146-150. doi:10.4319/lo.1973.18.1.0146

Dore, J.E., T. Houlihan, D.V. Hebel, G. Tien, L. Tupas and D.M. Karl. – 1996. Freezing as a method of sample preservation for the analysis of dissolved inorganic nutrients in seawater. Mar. Chem., 53: 173-185. doi:10.1016/0304-4203(96)00004-7

Duarte, C.M., S. Agustí, H. Kennedy and D. Vaqué. – 1999. The Mediterranean climate as a template for Mediterranean marine ecosystems: the example of the northeast Spanish littoral. Prog. Oceanogr., 44: 245-270. doi:10.1016/S0079-6611(99)00028-2

Emelianov, M., J. Font, A. Turiel, C. Millot, J. Solé, P.-M. Puolain, A. Julià and M.-R. Vitrià. – 2006. Transformation of Levantine Intermediate Water tracked by MEDARGO floats in the Western Mediterranean. Ocean Sci., 2: 281-290. doi:10.5194/os-2-281-2006

Eppley, R.W., J. H. Sharp, E.H. Renger, M.J. Perry and W.G. Harrison.– 1977. Nitrogen assimilation by phytoplankton and other microorganisms in surface waters of central North Pacific Ocean. Mar. Biol., 39: 111-120. doi:10.1007/BF00386996

Estrada, M. – 1999. Hidrodinàmica i fitoplàncton en el Mar Català. Memorias de la Real Academia de Ciencias y Artes de Barcelona, 961. Vol. LVIII, num. 6: 189-247.

Fichaut, M., E. Balopoulos, L. Baudet, H. Dooley, M.-J. García-Fernández, A. Iona, D. Jourdan and C. Maillard. – 1997. A common protocol to assemble a coherent database from distributed heterogeneous data sets: the MEDATLAS database experience. Proc. MAST workshop on project data management, June 1997, Ispra, Italy.

Garcia, H., A. Cruzado, L. Gordon and J. Escanez. – 1998. Decadal-scale chemical variability in the subtropical north Atlantic deduced from nutrient and oxygen data. J. Geophys. Res., 103: 2817-2830. doi:10.1029/97JC03037

Gomez-Taylor, M., H.D. Kahn, W.A. Telliard, K. Ditthavong, L. Kopylev, H. McCarty, L. Riddick, K. Miller, J. Cuddeback, D. Rushneck, S. Dedah, K. Stralka. – 2003. Technical Support Document for the Assessment of Detection and Quantitation Approaches. U.S. Environmental Protection Agency, Washington D.C.

Gordon, L.I., J.C. Jennings, Jr.A.A. Ross and J.M. Krest. – 1993. A suggested protocol for continuous flow automated analysis of seawater nutrients (phosphate, nitrate, nitrite and silicic acid) in the WOCE Hydrographic Program and the Joint Global Ocean Fluxes Study. WOCE Hydrographic Program Office, Methods Manual WHPO 91-1.

Grasshoff, K. – 1964. On the determination of silica in sea water. Deep-Sea Res., 11: 597-604.

Grasshoff, K. – 1970. Technicon paper, 691: 57.

Grasshoff, K., M. Ehrhardt and K. Kremling K. – 1983. Methods of seawater analysis. Verl. Chem., Weinheim.

PEPS Group. – 1986. Datos oceanográficos básicos de las campañas PEP-82, PEP-83 y PEP-84 en el mar Catalán. Datos Informativos Instituto de Ciencias del Mar, Barcelona, 19: 1-100.

Hager, S.W., E.L. Atlas, L.I. Gordon, A.W. Mantyla and P.K. Park. – 1972. A comparison at sea of manual and autoanalyzer analyses of phosphate, nitrate and silicate. Limnol. Oceanogr., 17(6): 931-937. doi:10.4319/lo.1972.17.6.0931

Hansen, H.P. – 1999. Determination of oxygen. In: K. Grasshoff, K. Kremling and M. Ehrhardt (eds.), Methods of Seawater Analysis. Wiley-VCH, 75-89.

Hansen, H.P. and F. Koroleff. – 1999. Determination of nutrients. In: K. Grasshoff, K. Kremling and M. Ehrhardt (eds.), Methods of Seawater Analysis. Wiley-VCH, 191-228.

Kremling, K. and L. Bru.gmann. – 1999. Filtration and Storage. In: K. Grasshoff, K. Kremling and M. Ehrhardt (eds.), Methods of Seawater Analysis. Wiley-VCH, 27-40.

Krom, M.D., E.M.S. Woodward, B. Herut, N. Kress, P. Carbo, R.F.C. Mantoura, G. Spyres, T.F. Thingstad, P. Wassmann, C. Wexels-Riser, V. Kitidis, C.S. Law and G. Zodiatis. – 2005. Nutrient cycling in the south east levantne basin of the Eastern Mediterranean: results from a phosphorus starved system. Deep-Sea Res. II., 52: 2879-2896. doi:10.1016/j.dsr2.2005.08.009

Lomas, M.W. and F. Lipschultz. – 2006. Forming the primary nitrite maximum: nitrifiers or phytoplankton? Limnol. Oceanogr., 51(5): 2453-2467. doi:10.4319/lo.2006.51.5.2453

Masó, M. and PEPS Group. – 1988. Datos oceanográficos básicos de las campañas Fronts-3-85, Fronts-6-85, PEP-86, Fronts-11-86 y PEP-87 en el mar Catalán. Datos Informativos Instituto de Ciencias del Mar, Barcelona, 24: 1-83.

Masó, M. and Varimed Group. – 1995. Datos Oceanográficos Básicos de las Campañas «FRONTS 1992» (octubre-noviembre 1992) y «Variabilidad de mesoescala en el Mediterráneo Occidental» (junio 1993). Datos Informativos Instituto de Ciencias del Mar, Barcelona, 27: 1-117.

Mater Group. – 2001. MTPII-MATER 1996–1999 (Mass transfer and ecosystem response) database. IFREMER Edition (1 CD).

Medar Group. – 2002. MEDATLAS/2002 database. Mediterranean and Black Sea database of temperature salinity and bio-chemical parameters. Climatological Atlas. IFREMER Edition (4 CD).

Millot, C. – 1999. Circulation in the western Mediterranean Sea. J. Mar. Sys., 20: 423-442. doi:10.1016/S0924-7963(98)00078-5

Morán, X.A.G., J.M. Gasol, L. Arin and M. Estrada. A comparison between glass fiber and membrane filters for the estimation of phytoplankton POC and DOC production. Mar. Ecol. Prog. Ser., 187: 31-41.

Moutin, T. and P. Raimbault. – 2002. Primary production, carbon export and nutrients availability in Western and Eastern Mediterranean Sea in early summer 1996 (MINOS cruise). J. Mar. Sys., 33: 273-288. doi:10.1016/S0924-7963(02)00062-3

Murphy, J. and J.P. Riley. – 1962. A modified single solution method for the determination of phosphate in natural waters. Anal. Chim. Acta, 27: 31-36. doi:10.1016/S0003-2670(00)88444-5

Parsons, T.R., M. Takahashi and B. Hargrave. – 1984. Biological Oceanographic Processes. Pergamon Press, Oxford.

Pond, S. and G.L. Pickard. – 1983. Introductory Dynamical Oceanography. Pergamon Press, Oxford.

Riley, J.P., D.E. Robertson, J.W.R. Dutton, N.T. Mitchell and P.J.leB. Williams. – 1975. Analytical chemistry of Sea Water. In: J.P. Riley and G. Skirrow (eds.), Chemical Oceanography. Vol.3, Academic Press, 193-433.

Riu, J., A. Maroto, R. Boqué, F.X. Rius. – 2002. Determinació de la traçabilitat en mesures químiques. Notícies per a Químics, 407: 5-8.

Salat, J. – 1995. The interaction between the Catalan and Balearic currents in the southern Catalan Sea. Oceanol. Acta, 18(2): 227-234.

Salat, J. – 1996. Review of hydrographic environmental factors that may influence habitats in northwestern Mediterranean. Sci. Mar., 60(Suppl. 2): 21-32.

Salat, J. and A. Cruzado. – 1981. Masses d’eau dans la Mediterranée Occidentale: Mer Catalane et eaux adjacentes. Rapp. Comm. Int. Mer Médit., 27(6): 201-209.

Send, U., J. Font, G. Krahmann, C. Millot, M. Rhein and J. Tintore. – 1999. Recent advances in observing the physical oceanography of the western Mediterranean Sea. Progr. Oceanogr., 44: 37-64. doi:10.1016/S0079-6611(99)00020-8

Stéfansson, U. and F. Richards. – 1963. Processes contributing to the nutrient distributions off the Columbia River and Strait of Juan de Fuca. Limnol. Oceanogr., 8: 394-410. doi:10.4319/lo.1963.8.4.0394

Strickland, J.D.H. and T.R. Parsons. – 1972. A Practical Handbook of Seawater Analysis. Fisheries Research Board Canada, 167: 1-310.

Thayer, G.W. – 1970. Comparison of two storage methods for the analysis of nitrogen and phosphorous fractions in estuarine water. Chesapeake Sci., 11: 155-158. doi:10.2307/1351238

Valderrama, J.C. – 1995. Methods of nutrient analysis. In: G.M. Hallegraeff, D.M. Anderson and A.D. Cembella (eds.), Manual of Harmful Marine Microalgae. UNESCO, Paris, 251-268.

Varela, R. and FRONTS Group. – 1991. Datos Oceanográficos Básicos de las Campañas FRONTS 1989, FRONTS 1990 y FRONTS 1991 en el Mar Catalán. Datos Informativos Instituto de Ciencias del Mar, Barcelona, 26: 1-71.

Velásquez, Z. – 1997. Fitoplancton en el mediterráneo noroccidental. Ph.D. thesis, Univ. Politècnica de Catalunya.

Venrick, E.L. and T.L. Hayward. – 1985. Evaluation of some techniques for preserving nutrients in stored seawater samples. California Cooperative Oceanic and Fisheries Investigations Report XXVI: 160-168.

Winkler, L.W. – 1888. Die Bestimmung des in Wasser gelosten Sauerstoffen. Berichte der Deutschen Chemischen Gesellschaft, 21: 2842-2855. doi:10.1002/cber.188802102122

Whitledge, T.E., S.T. Mallory, C.S. Patton and C.D. Wiritk. – 1981. Automated Analysis in Seawater. Nat. Tech. Inf. Serv. Springfield, Brookhaven Nat. Laboratory, USA.

Yentsch, C.S. and D.W. Menzel. – 1963. A method for the determination of phytoplankton chlorophyll and phaeophytin by fluorescence. Deep-Sea Res., 10: 221-231.

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Published

2011-06-30

How to Cite

1.
Segura-Noguera M, Cruzado A, Blasco D. Nutrient preservation, analysis precision and quality control of an oceanographic database of inorganic nutrients, dissolved oxygen and chlorophyll a from the NW Mediterranean Sea. Sci. mar. [Internet]. 2011Jun.30 [cited 2024Mar.29];75(2):321-39. Available from: https://scientiamarina.revistas.csic.es/index.php/scientiamarina/article/view/1255

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