@article{Sá-Pinto_Alexandrino_Branco_2007, title={High genetic differentiation with no evidence of hybridisation between four limpet species (Patella spp.) revealed by allozyme loci}, volume={71}, url={https://scientiamarina.revistas.csic.es/index.php/scientiamarina/article/view/490}, DOI={10.3989/scimar.2007.71n4801}, abstractNote={The occurrence of hybridisation between limpet species of the genus Patella has always been a contentious issue. Although a previous allozyme study reported high differentiation and no hybridisation between <em>Patella vulgata</em> Linnaeus, 1758, <em>Patella depressa</em> Pennant, 1777 and <em>Patella ulyssiponensis</em> Gmelin, 1791 along English shores, the recent finding of an mtDNA haplotype of <em>P. depressa</em> in a <em>P. vulgata</em> individual raised new doubts on this issue. To further study the possibility of hybridisation between limpet species and their level of genetic differentiation, ten allozyme loci were screened using starch gel electrophoresis for <em>P. ulyssiponensis, P. depressa, P. vulgata</em> and <em>Patella rustica</em> Linnaeus, 1758, from the Atlantic coast of the Iberian Peninsula. Our results show high differentiation between species, which could be clearly separated into different clusters with a Bayesian clustering algorithm. No significant signs of hybridisation were detected between any of the four species. Thus, the hypothesis of hybridisation between <em>P. vulgata</em> and <em>P. depressa</em> across their sympatric distribution is not supported. Two sympatric clusters were recovered within <em>P. vulgata</em> that could be related to Hardy-Weinberg disequilibrium found in locus MPI. Finally, due to the high level of intraspecific variability, the studied loci are interesting tools for the analysis of population structure and stock identification.}, number={4}, journal={Scientia Marina}, author={Sá-Pinto, Alexandra and Alexandrino, Paulo and Branco, Madalena}, year={2007}, month={Dec.}, pages={801–810} }