The application of species specific DNA-targeted probes and fluorescently tagged lectin to differentiate several species of Pseudo-nitzschia (Bacillophyceae) in Chinhae Bay, Korea

Authors

  • Eun Seob Cho Harmful Algal Research Division, National Fisheries Research and Development Institute
  • Jong Gyu Park The Institute of Environment Research, Inje University
  • Bong Cheol Oh Harmful Algal Research Division, National Fisheries Research and Development Institute
  • Yong Chul Cho Harmful Algal Research Division, National Fisheries Research and Development Institute

DOI:

https://doi.org/10.3989/scimar.2001.65n3207

Keywords:

Pseudo-nitzschia, differentiation, DNA probes, FITC-conjugated lectins

Abstract


We applied molecular methods using a DNA probe and fluorescently tagged lectins to discriminate toxic Pseudo-nitzschia multiseries from the Chinhae Bay for a Korean harmfulalgae monitoring program. From the binding activity of lectins, P. multistriata, P. subfraudulenta, P. pungens, P. multiseries and P. cuspidata bound ConA, whereas P. subpacifica and P. delicatissima did not. Ribosomal RNA-targeted oligonucleotide probes (muD1, puD1 and deD1) specifically reacted to P. multiseries, P. pungens and P. delicatissima, respectively, whereas auD1, frD1 and amD1 probes did not bind to P. multiseries, P. pungens, P. cuspidata, P. multistriata, P. subpacifica, P. subfraudulenta and P. delicatissima. In particular, fluorescent FITC-conjugated WGA specifically bound to P. multiseries but not to P. pungens, indicating that this is a desirable method for their rapid and easy discrimination. In addition, we tested a species-specific oligonucleotide DNA probe (muD1) using the whole cell hybridization filter tube system, and the WGA lectin probe to discriminate P. multiseries in the field. The oligonucleotide probe and fluorescent WGA bound specifically to P. multiseries and these labelled cells were correlated to label cell abundance. These results imply that DNA and lectin probes are apporopriate tools for counting P. multiseries and distinguishing morphologically similar Pseudo-nitzschia species in natural samples, therefore, these methods are especially pertinent since rapid separation and quantitative estimation of cell abundance of P. multiseries are now important for a routine harmful algae monitoring program in Korean waters.

Downloads

Download data is not yet available.

Downloads

Published

2001-09-30

How to Cite

1.
Seob Cho E, Gyu Park J, Cheol Oh B, Chul Cho Y. The application of species specific DNA-targeted probes and fluorescently tagged lectin to differentiate several species of Pseudo-nitzschia (Bacillophyceae) in Chinhae Bay, Korea. Sci. mar. [Internet]. 2001Sep.30 [cited 2024Mar.28];65(3):207-14. Available from: https://scientiamarina.revistas.csic.es/index.php/scientiamarina/article/view/654

Issue

Section

Articles