Scientia Marina, Vol 78, No 1 (2014)

Reproductive phase determination in male meagre (Argyrosomus regius, Sciaenidae): testis development and histologic corroboration of a gross anatomical scale

Nuno Prista
Centro de Oceanografia, Faculdade de Ciências da Universidade de Lisboa - Departamento de Biologia Animal, Faculdade de Ciências da Universidade de Lisboa - Instituto Português do Mar e da Atmosfera , Portugal

Leonel Gordo
Centro de Oceanografia, Faculdade de Ciências da Universidade de Lisboa - Departamento de Biologia Animal, Faculdade de Ciências da Universidade de Lisboa , Portugal

José Lino Costa
Centro de Oceanografia, Faculdade de Ciências da Universidade de Lisboa , Portugal

Maria José Costa
Centro de Oceanografia, Faculdade de Ciências da Universidade de Lisboa - Departamento de Biologia Animal, Faculdade de Ciências da Universidade de Lisboa , Portugal

Cynthia Jones
Center for Quantitative Fisheries Ecology, Old Dominium University , United States


Reproductive stage determination of male gonads has received sparse attention in fish biology literature with few studies detailing the building of gross anatomical- and histologic scales. The meagre (Argyrosomus regius) is one of the world’s largest sciaenids and supports a significant regional fishery in European and North African waters whose reproductive patterns are yet to be fully investigated. In the present study, we derive a macroscopic grading system for meagre testis using semi-quantitative graphs that feature the testis variability along the species size range and time of the year. We then describe the histological stages and reproductive phases of male testes and determine the extent to which they corroborate the anatomical scale. Our results indicate that gross anatomical analyses are accurate in assessments of the meagre spawning season but may not precisely distinguish the testes of well-mature fish and first spawning virgins. Furthermore, we show that milt expression varies widely with size and misclassifies as immature many smaller fish in spawning-capable condition. We discuss these findings in terms of their contribution to the understanding of testes development and the uncertainties involved in determining the size-at-maturity of male fish using gross anatomical scales.


histology; testes; reproduction; males; validation; Argyrosomus regius

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