Scientia Marina, Vol 77, No 1 (2013)

Paralytic shellfish toxins in the Atlantic horse mackerel (Trachurus trachurus) over a bloom of Gymnodinium catenatum: the prevalence of decarbamoylsaxitoxin in the marine food web

Sandra Lage
IPMA – Instituto Português do Mar e da Atmosfera , Portugal

Pedro Reis Costa
IPMA – Instituto Português do Mar e da Atmosfera , Portugal


This study reports the accumulation of paralytic shellfish toxins (PSTs) in Atlantic horse mackerel (Trachurus trachurus) over a bloom of the toxigenic dinoflagellate Gymnodinium catenatum. High levels of toxins, up to 4800 μg STXeq kg–1, were registered at the peak of the bloom (5.0 103 cells l–1). The suite of individual PSTs was examined. Decarbamoylsaxitoxin (dcSTX) and B1 constituted nearly 90% of toxins (on a molar basis) determined in mackerel. This profile of toxins markedly differs from the known profile of toxins produced by G. catenatum strains isolated from the Portuguese coast, which is dominated by N-sulfocarbamoyl toxins, in particular the C1+2 toxins. The prevalence of the potent dcSTX in the pelagic environment and its transfer through the marine food web is highlighted in this study. Atlantic horse mackerel is identified as a high potential vector of PSTs along the Portuguese coast. This fish species has a central position in the marine food web, being an important predator of zooplankton and at the same time an important diet item of top predators. This study reveals bioaccumulation values that are important for evaluating potential impacts of blooms of PST-producing dinoflagellates on marine ecosystems or their components, such as fish.


marine toxins; saxitoxin; pelagic fish; phycotoxins; harmful algal blooms

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Bakke M.J., Horsberg T.E. 2010. Kinetic properties of saxitoxin in Atlantic salmon (Salmo salar) and Atlantic cod (Gadus morhua). Comp. Biochem. Phys. C 152: 444-450.

Cabral H.N., Murta A. 2002. The diet of blue whiting, hake, horse mackerel and mackerel off Portugal. J. Appl. Ichthyol. 18: 14-23.

Castonguay M., Levasseur M., Beaulieu J-L., Grégoire F., Michaud S., Bonneau E., Bates S.S. 1997. Accumulation of PSP toxins in Atlantic mackerel: seasonal and ontogenic variations. J. Fish. Biol. 50: 1203-1213.

Costa P.R., Botelho M.J., Lefebvre K.A. 2010. Characterization of paralytic shellfish toxins in seawater and sardines (Sardina pilchardus) during blooms of Gymnodinium catenatum. Hydrobiologia 655: 89-97.

Costa P.R., Lage S., Barata M., Pousão-Ferreira P. 2011. Uptake, transformation, and elimination kinetics of paralytic shellfish toxins in white seabream (Diplodus sargus). Mar. Biol. 158: 2805-2811.

Costa P.R., Pereira P., Guilherme S., Barata M., Nicolau L., Santos M.A., Pacheco M., Pousão-Ferreira P. 2012. Biotransformation modulation and genotoxicity in white seabream upon exposure to paralytic shellfish toxins produced by Gymnodinium catenatum. Aquat. Toxicol. 106: 42-47. PMid:22057254

Ebert D.A. 1991. Diet of the seven gill shark Notorynchus cepedianus in the temperate coastal waters of southern Africa. S. Afr. J. Mar. Sci. 11: 565-572.

EFSA (European Food Safety Authority) 2009. Scientific opinion of the panel on contaminants in the food chain on a request from the European Commission on Marine biotoxins in shellfish -Saxitoxin group. The EFSA Journal 1019: 1-76.

Fire S., Pruden J., Couture D., Wang Z., Bottein M-Y.D., Haynes B.L., Knott T., Bouchard D., Lichtenwalner A., Wippelhauser G. (in press). Saxitoxin exposure in endangered fish stocks: association of a shortnose sturgeon Acipenser brevirostrum mortality event with a harmful algal bloom in Maine. Mar. Ecol. Progr. Ser.

Geraci J.R., Anderson D.M., Timperi R.J., St Aubin D.J., Early G.A., Prescott J.H., Mayo C.A. 1989. Humpback whales (Megaptera novaeangliae) fatally poisoned by dinoflagellate toxin. Can. J. Fisher. Aquat. Sci. 46: 1895-1898.

Hall S., Stricharz, G., Moczydlowski, E., Ravindran, A., Reichardt, P.B. 1990. The saxitoxins: sources, chemistry and pharmacology. In: Hall S., Stricharz G. (eds), Marine Toxins: Origin, Structure and Molecular Pharmacology. vol. 418. American Chemical Society Symposium Series, Washington, DC, pp. 29-65.

Hallegraeff G.M. 1993. A review of harmful algal blooms and their apparent global increase. Phycologia 32: 79-99.

Hasle G.R. 1978. Phytoplankton manual: the inverted microscope method. In: Sournia, A. (ed.), Monographs on Oceanic Methodology. Unesco, Paris, pp. 88-96.

Jester R.J., Baugh K.A., Lefebvre K.A. 2009. Presence of Alexandrium catenella and paralytic shellfish toxins in finfish, shellfish and rock crabs in Monterey Bay, California, USA. Mar. Biol. 156: 493-504.

Kwong R.W.M., Wang W.X., Lam P.K.S., Yu P.K.N. 2006. The uptake, distribution and elimination of paralytic shellfish toxins in mussels and fish exposed to toxic dinoflagellates. Aquat. Toxicol. 80: 82-91. PMid:16959334

Lagos N., Andrinolo D. 2000. Paralytic Shellfish Poisoning (PSP): Toxicology and Kinetics. In: Botana L.M. (ed.), Seafood and Freshwater Toxins: Mode of Action, Pharmacology and Physiology. New York, Marcel Dekker, Inc. pp. 203-215

Landsberg J.H. 2002. The effects of harmful algal blooms on aquatic organisms. Rev. Fisher. Sci. 10: 113-390.

Lawrence J.F., Niedzwiadek B. 2001. Quantitative determination of paralytic shellfish poisoning toxins in shellfish by using prechromatographic oxidation and liquid chromatography with fluorescence detection. J. AOAC Int. 84: 1099-1108. PMid:11501910

Lefebvre K.A., Elder N.E., Hershberger P.K., Trainer V.L., Stehr C.M., Scholz N.L. 2005. Dissolved saxitoxin causes transient inhibition of sensorimotor function in larval Pacific herring (Clupea harengus pallasi). Mar. Biol. 147: 1393-1402.

Llewellyn L.E. 2006. Saxitoxin, a toxic marine natural product that targets a multitude of receptors. Nat. Prod. Rep. 23: 200-222. PMid:16572228

Masó M., Garcés E. 2006. Harmful microalgae blooms (HAB); problematic and conditions that induce them. Mar. Pollut. Bull. 53: 620-630. PMid:17010385

Monteiro A., Costa P.R. 2011. Distribution and selective elimination of paralytic shellfish toxins in different tissues of Octopus vulgaris. Harmful Algae 10: 732-737.

Mortensen A.M. 1985. Massive fish mortalities in the Faroe Islands caused by a Gonyaulax excavata red tide. In: Anderson D.A., White A.W., Baden D.G. (eds), Toxic Dinoflagellates. New York, pp. 165-170.

Negri A.P., Bolch C.J.S., Geier S., Green D.H., Park T.G., Blackburn S.I. 2007. Widespread presence of hydrophobic paralytic shellfish toxins in Gymnodinium catenatum. Harmful Algae 6: 774-780.

Oshima Y., Blackburn S.I., Hallegraeff G.M. 1993. Comparative study on paralytic shellfish toxin profiles of the dinoflagellate Gymnodinium catenatum from three different countries. Mar. Biol. 116. 471-476.

Reyero M., Cacho E., Martínez A., Vázquez J., Marina A., Fraga S., Franco J.M. 1999. Evidence of saxitoxin derivatives as causative agents in the 1997 mass mortality of monk seals in the Cape Blanc Peninsula. Nat. Toxins 7: 311-315.<311::AID-NT75>3.0.CO;2-I

Robineau B., Gagné J.A., Fortier L., Cembella A.D. 1991. Potential impact of a toxic dinoflagellate (Alexandrium excavatum) bloom on survival of fish and crustacean larvae. Mar. Biol. 108: 293-301.

Samson J.C., Shumway S.E., Weis J.S. 2008. Effects of the toxic dinoflagellate, Alexandrium fundyense on three species of larval fish: a food-chain approach. J. Fish Biol. 72: 168-188.

Sephton D.H, Haya K., Martin J.L., Legresley M.M., Page F.H. 2007. Paralytic shellfish toxins in zooplankton, mussels, lobsters and caged atlantic salmon, Salmo salar, during a bloom of Alexandrium fundyense of Grand Manan Island, in the Bay of Fundy. Harmful Algae 5: 745-758.

Silva M.A. 1999. Diet of dolphins, Delphinus delphis, off the Portuguese continental coast. J. Mar. Biol. Assoc. U.K. 79: 531-540.

Silva S., Larsen J., Rangel I., Jonico V. 2011. Fish mortality associated with Alexandrium cf. fundyense in Lobito Bay, Angola. Harmful Algae News 44: 5-5

Vale C., Alfonso A., Vieytes M.R., Romarís X.M., Arévalo F., Botana A.M., Botana L.M. 2008. In vitro and in vivo evaluation of paralytic shellfish poisoning toxin potency and the influence of the pH of extraction. Anal. Chem. 80: 1770-1776. PMid:18232710

White A.W. 1977. Dinoflagellate toxins as probable cause of an Atlantic herring (Clupea harengus harengus) kill, and pteropods as apparent vector. J. Fish. Res. Bd. Can. 34: 2421- 2424.

White A.W., 1981. Sensitivity of marine fishes to toxins from the red-tide dinoflagellate Gonyaulax excavata and implications for fish kills. Mar. Biol. 65: 255-260.

Wiese M., D'Agostino P.M., Mihali T.K., Moffitt M.C., Neilan B.A. 2010. Neurotoxic alkaloids: saxitoxin and its analogs. Mar. Drugs 8: 2185-2211. PMid:20714432 PMCid:2920551

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