Oxidative stress biomarkers in the gills of the bivalve <em>Mactra stultorum</em> exposed to acrylamide

Wafa Trabelsi; Chaima Fouzai; Imene Chetoui; Safa Bejaoui; Khaoula Telahigue; Imen Rabeh; Mhamed El Cafsi; Nejla Soudani

doi:10.3989/scimar.04993.11A

Authors

Wafa Trabelsi Laboratory of Ecology, Biology and Physiology of Aquatic Organisms, Department of Biology, Tunis Faculty of Sciences, Univ. Tunis https://orcid.org/0000-0002-1114-4080
Chaima Fouzai Laboratory of Ecology, Biology and Physiology of Aquatic Organisms, Department of Biology, Tunis Faculty of Sciences, Univ. Tunis https://orcid.org/0000-0001-7588-1859
Imene Chetoui Laboratory of Ecology, Biology and Physiology of Aquatic Organisms, Department of Biology, Tunis Faculty of Sciences, Univ. Tunis https://orcid.org/0000-0002-2259-5397
Safa Bejaoui Laboratory of Ecology, Biology and Physiology of Aquatic Organisms, Department of Biology, Tunis Faculty of Sciences, Univ. Tunis https://orcid.org/0000-0002-7946-2763
Khaoula Telahigue Laboratory of Ecology, Biology and Physiology of Aquatic Organisms, Department of Biology, Tunis Faculty of Sciences, Univ. Tunis https://orcid.org/0000-0001-8841-9911
Imen Rabeh Laboratory of Ecology, Biology and Physiology of Aquatic Organisms, Department of Biology, Tunis Faculty of Sciences, Univ. Tunis https://orcid.org/0000-0002-0307-473X
Mhamed El Cafsi Laboratory of Ecology, Biology and Physiology of Aquatic Organisms, Department of Biology, Tunis Faculty of Sciences, Univ. Tunis https://orcid.org/0000-0002-9771-1110
Nejla Soudani Laboratory of Ecology, Biology and Physiology of Aquatic Organisms, Department of Biology, Tunis Faculty of Sciences, Univ. Tunis https://orcid.org/0000-0002-7652-9678

DOI:

https://doi.org/10.3989/scimar.04993.11A

Keywords:

acrylamide, exposure, Mactra stultorum, gills, antioxidant status, acetylcholinesterase

Abstract

Acrylamide (ACR) is among the most deleterious pollutants in the environment and presents a serious risk to humans and ecosystems. The purpose of this study was to assess its effects when administered at different concentrations (5, 10 and 20 mg L^–1) to evaluate antioxidant status in the gills of Mactra stultorum. Our results showed, after five days of treatment, an increase in malondialdehyde (MDA), lipid hydroperoxides (LOOH), advanced oxidation protein products (AOPP), reduced glutathione (GSH), ascorbic acid (Vit C) and metallothionein (MDA) levels in gills of treated clams compared with controls. Moreover, an increase in superoxide dismutase (SOD) and a significant decrease in glutathione peroxidase (GPx) activities were also observed. Acrylamide induced neurotoxicity, as evidenced by the inhibition of acetylcholinesterase (AChE) activity in a dose-dependent manner. Overall, our results indicated that oxidative stress may be considered one of the mechanisms behind acrylamide toxicity in bivalves, although the subject requires more research.

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Oxidative stress biomarkers in the gills of the bivalve Mactra stultorum exposed to acrylamide

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