Scientia Marina, Vol 76, No 2 (2012)

Loss of genetic variability in a hatchery strain of Senegalese sole (Solea senegalensis) revealed by sequence data of the mitochondrial DNA control region and microsatellite markers


https://doi.org/10.3989/scimar.03232.07E

Pablo Sánchez
Departament d’Enginyeria Agroalimentària i Biotecnologia, Universitat Politècnica de Catalunya - Centre de Referència de Recerca i Desenvolupament en Aqüicultura de la Generalitat de Catalunya , Spain

Jordi Viñas
Department of Marine Biology, Texas A&M University at Galveston - Laboratori d’Ictiologia Genètica, Departament de Biologia, Universitat de Girona , Spain

Jaime R. Alvarado Bremer
Department of Marine Biology, Texas A&M University at Galveston - Department of Wildlife and Fisheries Sciences, Texas A&M University , United States

Pedro Pablo Ambrosio
Departament d’Enginyeria Agroalimentària i Biotecnologia, Universitat Politècnica de Catalunya - Centre de Referència de Recerca i Desenvolupament en Aqüicultura de la Generalitat de Catalunya , Spain

Rosa Flos
Departament d’Enginyeria Agroalimentària i Biotecnologia, Universitat Politècnica de Catalunya - Centre de Referència de Recerca i Desenvolupament en Aqüicultura de la Generalitat de Catalunya , Spain

Abstract


Comparisons of the levels of genetic variation within and between a hatchery F1 (FAR, n=116) of Senegalese sole, Solea senegalensis, and its wild donor population (ATL, n = 26), both native to the SW Atlantic coast of the Iberian peninsula, as well as between the wild donor population and a wild western Mediterranean sample (MED, n=18), were carried out by characterizing 412 base pairs of the nucleotide sequence of the mitochondrial DNA control region I, and six polymorphic microsatellite loci. FAR showed a substantial loss of genetic variability (haplotypic diversity, h=0.49±0.066; nucleotide diversity, π=0.006±0.004; private allelic richness, pAg=0.28) to its donor population ATL (h=0.69±0.114; π=0.009±0.006; pAg=1.21). Pairwise FST values of microsatellite data were highly significant (P < 0.0001) between FAR and ATL (0.053) and FAR and MED (0.055). The comparison of wild samples revealed higher values of genetic variability in MED than in ATL, but only with mtDNA CR-I sequence data (h=0.948±0.033; π=0.030±0.016). However, pairwise ΦST and FST values between ATL and MED were highly significant (P < 0.0001) with mtDNA CR-I (0.228) and with microsatellite data (0.095), respectively. While loss of genetic variability in FAR could be associated with the sampling error when the broodstock was established, the results of parental and sibship inference suggest that most of these losses can be attributed to a high variance in reproductive success among members of the broodstock, particularly among females.

Keywords


Solea senegalensis; genetic variability; mitochondrial DNA Control Region; microsatellite loci; broodstock management; variance in reproductive success; flatfish

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References


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