Scientia Marina, Vol 74, No 4 (2010)

Spermiogenesis particularities of a sperm storage species: Helicolenus dactylopterus (Teleostei: Scorpaenidae)


https://doi.org/10.3989/scimar.2010.74n4697

Silvia Vila
Departament de Ciències Ambientals, Universitat de Girona , Spain

Maria Sàbat
Departament de Ciències Ambientals, Universitat de Girona , Spain

Marta Muñoz
Departament de Ciències Ambientals, Universitat de Girona , Spain

Margarida Casadevall
Departament de Ciències Ambientals, Universitat de Girona , Spain

Abstract


A study of the spermiogenesis and spermatozoa of Helicolenus dactylopterus was conducted. Females of this species have the capacity to store sperm within their ovaries, and male gametes have a considerable cytoplasmic mass surrounding their heads to survive the long period of intraovarian sperm storage. Our observations show that early spermatids are round-shaped cells and have a spherical nucleus with diffuse chromatin. The nuclear volume decreases as a result of progressive chromatin condensation during spermiogenesis, causing the nucleus to take on a U-shape. Flagellar insertion is not central to the nucleus but consistently occurs at an oblique angle towards one side of it. The flagellum is inserted into the nuclear fossa, without subsequent nuclear rotation. In mature spermatozoa, the flagellum is adjacent to the nucleus. A comparison of the spermatozoa in the testicular lobules and those in the intraovarian storage structures suggests that the increase in volume of the cytoplasmic mass may occur in the posterior region of the testis, in the testicular duct. Spermatozoa enter the ovary in groups that reach the ovarian lumen and are surrounded by the ovarian epithelium for storage in sperm storage crypts.

Keywords


Helicolenus dactylopterus; spermiogenesis; ultrastructure; sperm storage; spermatozoa

Full Text:


PDF

References


Alberts, B., D. Bray, J. Lewis, M. Raff, K. Roberts and J.D. Watson. – 1996. Tráfico vesicular mediante las rutas secretora y endocítica. Biología molecular de la célula. 3rd ed., pp. 642-692. Ediciones Omega, Barcelona.

Allain, V. and P. Lorance. – 2000. Age estimation and growth of some deep-sea fish from the northeast Atlantic Ocean. Cybium, 24(Suppl. 3): 7-16.

Atwood, D.L. and F.S. Chia. – 1974. Fine structure of an unusual spermatozoon of a brooding sea cucumber, Cucumaria lubrica. Can. J. Zool., (52): 519. doi:10.1139/z74-064 PMid:4832969

Baccetti B. and B.A. Afzelius. – 1976. The biology of the sperm cell. Monogr. Dev. Biol., 10: 1-254. PMid:1107820

Burns, J.R., S.H. Weitzman, H.J. Grier and N.A. Menezes. – 1995. Internal fertilization, testis and sperm morphology in glandulocaudine fishes (Teleostei: Characidae: Glandulocaudinae). J. Morph., 224: 131-145. doi:10.1002/jmor.1052240203

DelaRoche, F.E. – 1809. Suite du mémoire sur les espèces de poissons observées à Iviça. Observations sur quelques-uns des poissons indiqués dans le précédent tableau et descriptions des espèces nouvelles ou peu connues. Ann. Mus. Hist. Nat. Paris (13): 313-361.

Estácio, S., A. Mendonça, H.M. Krug, G.M. Menezes, J. Branco and M.R. Pinho. – 2001. Aspects of the reproduction of six demersal species captured in the Azores archipelago. Arquipélago, Life Mar. Sci., 2B: 83-94.

Gardiner, D.M. – 1978. Cyclic changes in fine structure of the epithelium lining the ovary of the viviparous teleost Cymatogaster aggregata (Perciformes: Embiotocidae). J. Morph., 156: 367-380. doi:10.1002/jmor.1051560304 PMid:671554

Hayashi, S. – 1990. Studies of the ultrastructural changes of ovarian tissue with oogenesis and pregnancy in white-edged rockfish, Sebastes taczanowskii. Master’s Thesis, Hokkaido Univ., Hakodate, Japan.

Isidro, E.J. – 1989. Crescimento e reprodução de Helicolenus dactylopterus (DelaRoche, 1809). Relatório no âmbito das Provas de Aptidão de Acesso à categoria de Assistente de Investigação, Universidade dos Açores (Departamento de Oceanografia e Pescas), Horta, Faial, Açores.

Jamieson, B.G.M. – 1987. A biological classification of sperm types, with special reference to annelids molluscs, and an example of spermiocladistics. In: Mohri, H. (eds.), New Horizons in Sperm Cell Research, pp. 331-332. Gordon and Breach Science Publishers, New York.

Jamieson, B.G.M. and L.K.P. Leung. – 1991. Introduction to fish spermatozoa and the micropyle. In: Jamieson B.G.M. (eds.), Fish Evolution and Systematics: Evidence from spermatozoa. pp. 56-72. Cambridge University Press, Cambridge.

Kelly, C.J., P.L. Connolly and J.J. Bracken. – 1999. Age estimation, growth, maturity, and distribution of the bluemouth rockfish Helicolenus dactylopterus (DelaRoche, 1809) from the Rockall Trough. ICES J. Mar. Sci., 56(1): 61-74. doi:10.1006/jmsc.1998.0426

Koya, Y., H. Munehara and K. Takano. – 1997. Sperm storage and degradation in the ovary of a marine sculpin, Alcichthys alcicornis (Teleostei: Scorpaeniformes): role of intercellular junctions between inner ovarian epithelial cells. J. Morph., 233: 153-163. doi:10.1002/(SICI)1097-4687(199708)233:2<153::AID-JMOR5>3.0.CO;2-7

Koya, Y., H. Munehara and K. Takano. – 2002. Sperm storage and motility in the ovary of the marine sculpin Alcichthys alcicornis (Teleostei: Scorpaeniformes), with internal gametic association. J. Exp. Zool., 292: 145-155. doi:10.1002/jez.1150 PMid:11754030

Mattei, X. – 1970. Spermiogenèse comparée des poissons. In: B. Baccetti (eds.), Comparative Spermatology. pp. 57-72. Academic Press, New York.

Mendonça, A., E. Isidro, G. Menezes, M.R. Pinho, O. Melo and S. Estácio. – 2006. New contribution to the reproductive features of bluemouth Helicolenus dactylopterus dactylopterus from the northeast Atlantic (Azores Archipelago). Sci. Mar., 70(4): 679-688.

Muñoz, M., M. Casadevall and S. Bonet. – 1999. Annual reproductive cycle of Helicolenus dactylopterus dactylopterus (Teleostei: Scorpaeniformes) with special reference to the ovaries sperm storage. J. Mar. Biol. Assoc. U.K., 79: 521-529. doi:10.1017/S0025315498000642

Muñoz, M., M. Casadevall, S. Bonet and I. Quagio-Grassiotto. – 2000. Sperm storage structures in the ovary of Helicolenus dactylopterus dactylopterus (Teleostei: Scorpaenidae): an ultrastructural study. Env. Biol. Fishes, 58: 53-59. doi:10.1023/A:1007648111239

Muñoz, M., M. Casadevall and S. Bonet. – 2002a. Gametogenesis of Helicolenus dactylopterus dactylopterus (Teleostei: Scorpaenidae). Sarsia, 87(2): 119-127. doi:10.1080/003648202320205193

Muñoz, M., Y. Koya and M. Casadevall. – 2002b. Histochemical analysis of sperm storage in Helicolenus dactylopterus dactylopterus (Teleostei: Scorpaenidae). J. Exp Zool., 292: 156-164. doi:10.1002/jez.1151 PMid:11754031

Muñoz, M., Y. Koya and M. Casadevall. – 2002c. Histochemical analysis of sperm storage in Helicolenus dactylopterus dactylopterus (Teleostei: Scorpaeniformes). J. Exp. Zool., 292: 156-164. doi:10.1002/jez.1151 PMid:11754031

Quaggio-Grassiotto, I. and C. Oliveira. – 2008. Sperm ultrastructure and a new type of spermiogenesis in two species of Pimelodidae, with a comparative review of sperm ultrastructure in Siluriformes (Teleostei: Ostariophysi). Zool. Anz., 247(1): 55-66. doi:10.1016/j.jcz.2007.07.002

Quagio-Grassiotto, I., M.A. Spadella, M. Carvalho and C. Oliveira. – 2005. Comparative description and discussion of spermiogenesis and spermatozoal ultrastructure in some species of Heptapteridae and Pseudopimelodidae (Teleostei: Siluriformes). Neotr. Ichthyol., 3(3): 401-410. doi:10.1590/S1679-62252005000300008

Sequeira, V., I. Figueiredo, M. Muñoz and L.S. Gordo. – 2003. New approach to the reproductive biology of Helicolenus dactylopterus. J. Fish Biol., 62: 1206-1210. doi:10.1046/j.1095-8649.2003.00063.x

Takemura, A., K. Takano and H. Takahashi. – 1987. Reproductive cycle of a viviparous fish, white-edged rockfish, Sebastes taczanowskii. Bull. Fac. Hokkaido Univ., 38: 111-125.

Vila, S. – 2004. L’emmagatzematge d’esperma en Helicolenus dactylopterus dactylopterus (Teleostei: Scorpaenidae). DEA, Univ. Girona.

Vila, S. – 2007. Annual cycle of stored spermatozoa within the ovaries of Helicolenus dactylopterus dactylopterus (Teleostei, Scorpaenidae). J. Fish Biol., 71: 596-609. doi:10.1111/j.1095-8649.2007.01525.x

Washington, B.B., H.G. Moser, W.A. Laroche and W.J. Richards. – 1984. Scorpaeniformes: development. In: H.G. Moser, W.J. Richards, D.M. Cohen, M.P. Fahay, A.W. Kendall, Jr. and S.L. Richardson (eds.), Ontogeny and Systematics of Fishes. Special Publication Number 1, American Society of Ichthyologists and Herpetologists. pp. 405-428. Gainesville, Florida.

White, D.B., D.M. Wyanski and G.R. Sedberry. – 1998. Age, growth, and reproductive biology of the blackbelly rosefish from the Carolinas, U.S.A. J. Fish Biol., 53: 1274-1291. doi:10.1111/j.1095-8649.1998.tb00248.x

Whitehead, P.J.P., M.L. Bauchot, J.C. Hureau, J. Nielsen and E. Tortonese (eds.). – 1986. Fishes of the North-eastern Atlantic and the Mediterranean. UNESCO, Paris.

Wourms, J.P., B.D. Grove and J. Lombardi. – 1988. The maternal-embryonic relationship in viviparous fishes. In: W.S. Hoar and D.J. Randall (eds.), Fish Physiol., (11B): 1-134. Academic Press, San Diego.




Copyright (c) 2010 Consejo Superior de Investigaciones Científicas (CSIC)

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.


Contact us scimar@icm.csic.es

Technical support soporte.tecnico.revistas@csic.es