Scientia Marina, Vol 74, No 3 (2010)

Relationships between otolith size and fish size in some mesopelagic and bathypelagic species from the Mediterranean Sea (Strait of Messina, Italy)

Pietro Battaglia
ISPRA (Higher Institute for Environmental Protection and Research), Laboratory of Milazzo , Italy

Danilo Malara
ISPRA (Higher Institute for Environmental Protection and Research), Laboratory of Milazzo , Italy

Teresa Romeo
ISPRA (Higher Institute for Environmental Protection and Research), Laboratory of Milazzo , Italy

Franco Andaloro
ISPRA, Palermo, c/o Residence Marbela , Italy


The length-weight relationships and the regressions between otolith size (length and width) and fish length of some mesopelagic and bathypelagic fishes living in the central Mediterranean Sea were provided. Images and morphological description of otoliths (sagittae) from 16 species belonging to the families of Gonostomatidae (1), Microstomatidae (2), Myctophidae (8), Phosichthyidae (2), Sternoptychidae (2) and Stomiidae (1) were given. The length-weight relationship showed an isometric growth in 13 species. No differences between right and left otolith sizes were detected by t-test, so a single linear regression was plotted against standard length (SL) for otolith length (OL) and otolith width (OW). Data fitted well to the regression model for both OL and OW to SL, for each species (R2 > 0.8). These relationships offer a helpful tool in feeding studies and also provide support to palaeontologists in their research on fish fossils.


mesopelagic fishes; bathypelagic fishes; otolith; fish-otolith sizes; length-weight relationship; Mediterranean Sea

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Abrams, P.A., B.A. Menge, G.G. Mittelbach, D.A. Spiller and P. Yodzis. – 1996. The role of indirect effects in food webs. In: G.A. Polis and K.O. Winemiller (eds), Food Webs. Integration of pattern and dynamics, pp. 371-395. New York, Chapman and Hall.

Alverson, F.G. – 1963. The food of yellowfin and skipjack tunas in the eastern tropical Pacific Ocean, Inter-Am. Trop. Tuna Comm. Bull., 7: 295–396.

Anderson, R.O. and R.M. Neumann. – 1996. Length, weight and associated structural indices. In: B.R. Murphy and D. Wills (eds.), Fisheries Techniques, pp. 447-481. Bethesda, American Fisheries Society.

Benoit-Bird K.J. and W.W.L. Au. – 2003. Echo strength and density structure of Hawaiian mesopelagic boundary community patches. J. Acoust. Soc. Am., 114 (4): 1888-1897. doi:10.1121/1.1612484 PMid:14587589

Berdar, A., G. Cavallaro, G. Giuffrè and A. Potoschi. – 1977. Contributo alla conoscenza dei Pesci spiaggiati lungo il litorale siciliano dello Stretto di Messina. Mem. Biol. Mar. Oceanogr., 7(5-6): 77-87.

Campana, S.E. and J.M. Casselman. – 1993. Stock discrimination using otolith shape analysis. Can. J. Fish. Aquat. Sci., 50: 1062-1083. doi:10.1139/f93-123

Campana, S.E. – 2004. Photographic atlas of fish otoliths of the Northwest Atlantic Ocean, pp. 284. ••••Ottawa, Ontario: NRC Research Press.

Castriota, L., C. Pipitone, S. Campagnuolo, M. Romanelli, A. Potoschi and F. Andaloro. – 2007. Diet of Coryphaena hippurus (Coryphaenidae) associated with FADs in the Ionian and Southern Tyrrhenian Seas. Cybium, 31: 435-441.

Consoli, P., T. Romeo, P. Battaglia, L. Castriota, V. Esposito and F. Andaloro. – 2008. Feeding habits of the albacore tuna Thunnus alalunga (Perciformes, Scombridae) from central Mediterranean Sea. Mar. Biol., 155: 113-120. doi:10.1007/s00227-008-1012-1

De Domenico, E. – 1987. Caratteristiche fisiche e chimiche delle acque nello Stretto di Messina. In: Di Geronimo, Barrier and Mantenat, (eds.), Le Detroit de Messine, Evolution Tectono-Sedimentaire Recente (Pliocene et Quaternaire) et Environment Actuel. Documents et Travaux de l’IGAL, Paris, 11: 225-235.

Dolar, M.L.L., W.A. Walker, G.L. Kooyman and W.F. Perrin. – 2003. Comparative feeding ecology of spinner dolphins (Stenella longirostris) and Fraser’s dolphins (Lagenodelphis hosei) in the Sulu Sea. Mar. Mamm. Sci., 19: 1-19. doi:10.1111/j.1748-7692.2003.tb01089.x

Donato, A., E. Pizzul, L. Salpietro, M. Specchi and G. Valli. – 1993. Osservazioni sulla biologia di Argyropelecus hemigymnus Cocco (1829) (Osteichthyes, Sternoptychidae). Suppl. Ric. Biol. Selvaggina, 21: 497-505.

Duffy, D.C. and L.J.B. Laurenson. – 1983. Pellets of cape cormorants as indicators of diet. Condor 85: 305-307. doi:10.2307/1367066

Falautano, M., L. Castriota, M.G. Finoia and F. Andaloro. – 2007. Feeling ecology of little tunny Euthynnus alletteratus in the central Mediterranean Sea. J. Mar. Biol. Ass. U.K., 87: 999-1005. doi:10.1017/S0025315407055798

Genovese, S., A. Berdar and L. Guglielmo. – 1971. Spiaggiamenti di fauna abissale nello Stretto di Messina. Atti Soc. Pelor. Sci., 17: 331-370.

Gamboa, D.A. – 1991. Otolith Size versus Weight and Body-Length Relationships for Eleven Fish Species of Baja California, Mexico. Fish. Bull. U.S., 89: 701-706.

Girone, A., D. Nolf and H. Cappetta. – 2006. Pleistocene fish otoliths from the Mediterranean Basin: a synthesis. Geobios, 39: 651-671. doi:10.1016/j.geobios.2005.05.004

Gjøsaeter, J. and K. Kawaguchi. – 1980. A review of the world resources of mesopelagic fish. FAO Fish. Tech. Pap., 193, FIRM/TI93, pp. 151.

Granadeiro, J.P. and M.A. Silva. – 2000. The use of otoliths and vertebrae in the identification and size-estimation of fish in predator-prey studies. Cybium, 24: 383-393.

Harvey, J.T., T.R. Loughlin, M.A. Perez and D.S. Oxman. – 2000. Relationship between fish size and otolith length for 63 species of fishes from the eastern north Pacific Ocean. NOAA Tech. Rep. NMFS, 150, pp. 35.

Hassani, S., L. Antoine and V. Ridoux. – 1997. Diets of albacore, Thunnus alalunga, and dolphins, Delphinus delphis and Stenella coerulaeoalba, caught in the northeast Atlantic albacore drift-net fishery: A progress report. J. Northwest Atl. Fish. Sci., 22: 119-123. doi:10.2960/J.v22.a10

lkyaz, A.T., G. Metin, O. Soykan and H.T. Kinacigil. – 2008. Length–weight relationship of 62 fish species from the central Aegean Sea, Turkey. J. Appl. Ichthyol., 24: 699-702.

Jobling, M. and A. Breiby - 1986. The use and abuse of fish otoliths in studies of feeding habits of marine piscivores. Sarsia, 71: 265-274.

Johnstone, I.G., M.P. Harris, S. Wanless and J.A. Graves. – 1990. The usefulness of pellets for assessing the diet of adult shags Phalacrocorax aristotelis. Bird Study, 37: 5-11. doi:10.1080/00063659009477030

Karakulak, F.S., A. Salman and I.K. Oray. – 2009. Diet composition of bluefin tuna (Thunnus thynnus L. 1758) in the Eastern Mediterranean Sea, Turkey. J. Appl. Ichthyol., in press.

Kristoffersen, J.B. – 2007. Growth rate and relative otolith size in populations of adult Mu.ller’s pearlside Maurolicus muelleri. J. Fish Biol., 71: 1317-1330. doi:10.1111/j.1095-8649.2007.01593.x

Lam, V. and D. Pauly. – 2005. Mapping the global biomass of mesopelagic fishes. Sea Around Us Project Newsletter July/August 30: 4.

Lebourges-Dhaussy, A., E. Marchal, C. Menkès, G. Champalbert and B. Biessy. – 2000. Vinciguerria nimbaria (micronekton), environment and tuna: their relationships in the Eastern Tropical Atlantic. Oceanol. Acta, 23: 515-528. doi:10.1016/S0399-1784(00)00137-7

Linkowski, T.B. – 1991. Otolith microstructure and growth patterns during the early life history of lanternfishes (family Myctophidae). Can. J. Zool., 69: 1777-1792. doi:10.1139/z91-247

Lombarte, A., Ò. Chic, V. Parisi-Baradad, R. Olivella, J. Piera and E. García-Ladona. – 2006. A web-based environment from shape analysis of fish otoliths. The AFORO database ( Sci. Mar., 70: 147-152.

Mann, K.H. – 1984. Fish production in open ocean systems. In: M.J.R. Fasham (ed.), Flows of energy and materials in marine ecosystems. Theory and practice, pp. 435-458. New York, Plenum Press.

Marabello, F., L. Guglielmo, A. Granata and O. Sidoti. – 1996. •Studi preliminari sulle abitudini alimentari di Todarodes sagittatus (Cephalopoda) nel Tirreno meridionale. In: G. Albertelli, A. De Maio and M. Piccazzo (eds.), Atti 11° Congresso AIOL (Ass. It. Oceanol. Limnol.), pp. 271-278. Genova, Italy.

Marshall, N.B. – 1960. Swimbladder structure of deep-sea fishes in relation to their systematics and biology. Disc. Rep., 31: 3-122.

Mata, A.J., J. Morales and L. Márquez. – 2008. Weight–length relationships for 26 demersal fish species of the Spanish South-Atlantic coastal waters. J. Appl. Ichthyol., 24: 330-333. doi:10.1111/j.1439-0426.2008.01058.x

Mazzarelli, G. – 1909. Gli animali abissali e le correnti sottomarine dello Stretto di Messina. Riv. Mens. Pesca Idrobiol., 11: 177-218.

Mostarda, E., D. Campo, L. Castrista, V. Esposito, M.P. Scarabello and F. Andaloro. – 2007. Feeling habits of the bullet tuna Auxis rochei in the southern Tyrrhenian Sea. J. Mar. Biol. Ass. U.K., 87: 1007-1012. doi:10.1017/S0025315407055440

Moteki, M., M. Arai, K. Tsuchiya and H. Okamoto. – 2001. Composition of piscine prey in the diet of large pelagic fish in the eastern tropical Pacific Ocean. Fish. Sci., 67: 1063-1074. doi:10.1046/j.1444-2906.2001.00362.x

Nishimura, A and J. Yamada. – 1988. Geographical differences in early growth of walleye Pollock Theragra chalcogramma, estimated by back-calculation of otolith daily growth increments. Mar. Biol., 97: 459-465. doi:10.1007/BF00391041

Nolf, D. – 1985. Handbook of Paleoichthyology. Otolithi piscium. 10, pp. 141 Stuttgart, Springer Verlag.

Nolf, D. – 1995. Studies on fossil otoliths — the state of the art. In: D.H. Secor, J.M. Dean and S.E. Campana, (eds.). Recent developments in fish otolith research, pp. 513-544. University of South Carolina Press.

Ohizumi, H., T. Kuramochi, T. Kubodera, M. Yoshioka and N. Miyazaki. – 2003. Feeding habits of Dall’s porpoises (Phocoenoides dalli) in the subarctic North Pacific and the Bering Sea basin and the impact of predation on mesopelagic micronekton. Deep-Sea Res. I, 50: 593-610. doi:10.1016/S0967-0637(03)00033-5

Pauly, D., A.W. Trites, E. Capuli and V. Christensen. – 1998. Diet composition and trophic levels of marine mammals. ICES J. Mar. Sci., 55: 467-481. doi:10.1006/jmsc.1997.0280

Pierce, G.J. and P.R. Boyle. – 1991. A review of methods for diet analysis in piscivorous marine mammals. Oceanogr. Mar. Biol. Ann. Rev. (London), 29: 409-486.

Pierce, G.J., P.R. Boyle and J.S.W. Diack. – 1991. Identification of fish otoliths and bones in faeces and digestive tracks of seals. J. Zool. (London), 224: 320-328. doi:10.1111/j.1469-7998.1991.tb04810.x

Potoschi, A., P. Battaglia, G. Rossi and M. Triscari. – 2003. Studio sulla crescita e su alcuni aspetti biologici di Hygophum benoiti (Cocco, 1838), Pisces, Myctophidae. Biol. Mar. Medit., 10: 127-137.

Rasmussen, O.I. and J. Giske. – 1994. Life-history parameters and vertical distribution of Maurolicus muelleri in Masfjorden in summer. Mar. Biol., 120: 649-664. doi:10.1007/BF00350086

Reichenbacher, B., E. Kamrani, H.R. Esmaeili and A. Teimori. – 2009. The endangered cyprinodont Aphanius ginaonis (Holly, 1929) from southern Iran is a valid species: evidence from otolith morphology. Environ. Biol. Fish., in press. doi:10.1007/s10641-009-9549-5

Romeo, T., P. Consoli, L. Castriota and F. Andaloro. – 2009. An evaluation of resource partitioning between two billfish, Tetrapturus belone and Xiphias gladius, in the central Mediterranean Sea. J. Mar. Biol. Ass. U.K., 89: 839-848. doi:10.1017/S0025315408002087

Salvanes, A.G.V. and J.B. Kristoffersen. – 2001. Mesopelagic fishes. In: J.H. Steele, S.A. Thorpe and K.K. Turekian (eds.), Encyclopedia of ocean sciences, 3, pp. 1711-1717. Academic Press.

Salvanes, A.G.V. and B.M. Stockley. – 1996. Spatial variation of growth and gonadal developments of Maurolicus muelleri in the Norwegian Sea and in a Norwegian fjord. Mar. Biol., 126: 321-332. doi:10.1007/BF00347456

Santos, M.B., M.R. Clarke and G.J. Pierce. – 2001. Assessing the importance of cephalopods in the diets of marine mammals and other top predators: problems and solutions. Fish. Res., 121-129. doi:10.1016/S0165-7836(01)00236-3

Sinopoli, M., C. Pipitone, S. Campagnuolo, D. Campo, L. Castriota, E. Mostarda and F. Andaloro. – 2004. Diet of young-of-the-year bluefin tuna, Thunnus thynnus (Linnaeus, 1758), in the southern Tyrrhenian (Mediterranean) Sea. J. Appl. Ichthyol., 20: 310-313. doi:10.1111/j.1439-0426.2004.00554.x

Smale, M.J., G. Watson and T. Hecht. – 1995. Otolith atlas of southern African marine fishes. Ichthyol. Monogr. JLB Smith Inst. Ichthyol, 1(14), pp. 253.

Snedecor, G.W. and W.G. Cochran. – 1967. Statistical Methods, 6th edn., pp. 593. Ames, Iowa, USA, The Iowa State Univ. Press.

Spalletta, B., G. Mento, F. Costa, G. Ammendolia, D. Giordano, D. Capecchi and A. Berdar. – 1995. Importanza dello spiaggiamento nella raccolta di specie ittiche rare ed endemiche dello Stretto di Messina (cenni di parassitosi). Riv. Parassitol., XII (LVI), 2: 279-297.

Springer, A.M., J.F. Piatt, V.P. Shuntov, G.B. Van Vliet, V.L. Vladimirove, A.E. Kuzin and A.S. Perlov. – 1999. Marine birds and mammals of the Pacific Subarctic Gyres. Progr. Oceanogr., 43: 443-487. doi:10.1016/S0079-6611(99)00014-2

Tortonese, E. – 1970. Osteichthyes (Pesci ossei). Parte prima. In: Calderini (ed.), Fauna d’Italia, X, pp. 565. Bologna.

Tuset, V.M., A. Lombarte and C.A. Assis. – 2008. Otolith atlas for the western Mediterranean, north and central eastern Atlantic. Sci. Mar., 72S1: 7-198.

Valle, C., J.T. Bayle and A.A. Ramos. – 2003. Weight-length relationships for selected fish species of the western Mediterranean Sea. J. Appl. Ichthyol., 19: 261-262. doi:10.1046/j.1439-0426.2003.00492.x

Vercelli, F. – 1925. Crociere per lo studio dei fenomeni dello Stretto di Messina. I. Il regime delle correnti e delle maree nello Stretto di Messina, pp. 209. Commissione Internazionale del Mediterraneo, Venezia, Off. Grafiche Ferrari.

Waessle, J.A., C.A. Lasta and M. Bavero. – 2003. ••Otolith morphology and body size relationships for juvenile Sciaenidae in the Río de la Plata estuary (35-36°S). Sci. Mar., 67: 233-240.

Watanabe, H., T. Kubodera, T. Ichii and S. Kawahara. – 2004. Feeding habits of neon flying squid Ommastraphes bartramii in the transitional region of the central North Pacific. Mar. Ecol. Progr. Ser., 266: 173-184. doi:10.3354/meps266173

Whitehead, P.J.P., M.L. Bauchot, J.C. Hureau, J. Nielsen and E. Tortonese (eds.). – 1984-1986. Fishes of the north-eastern Atlantic and the Mediterranean. UNESCO, Paris.

Williams, T. and B.C. Bedford. – 1974. The use of otoliths for age determination. In: T.B. Bagenal (ed.), The ageing of fish, pp. 114-123. Surrey, England, Unwin Brothers.

Wyllie Echeverria, T. – 1987. Relationship of otolith length to total length in rockfishes from northern and central California. Fish. Bull., 85: 383-387.

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