Scientia Marina, Vol 74, No 3 (2010)

DNA extraction from formalin-fixed tissue: new light from the deep sea


https://doi.org/10.3989/scimar.2010.74n3465

Ferran Palero
Evolutionary Genetics, Institute of Science and Technology Austria (ISTA), Austria

Sally Hall
National Oceanography Centre, Southampton, School of Ocean and Earth Science, University of Southampton, United Kingdom

Paul F. Clark
Department of Zoology, Natural History Museum, United Kingdom

David Johnston
Department of Zoology, Natural History Museum, United Kingdom

Jackie Mackenzie-Dodds
Department of Zoology, Natural History Museum, United Kingdom

Sven Thatje
National Oceanography Centre, Southampton, School of Ocean and Earth Science, University of Southampton, United Kingdom

Abstract


DNA samples were extracted from ethanol and formalin-fixed decapod crustacean tissue using a new method based on Tetramethylsilane (TMS)-Chelex. It is shown that neither an indigestible matrix of cross-linked protein nor soluble PCR inhibitors impede PCR success when dealing with formalin-fixed material. Instead, amplification success from formalin-fixed tissue appears to depend on the presence of unmodified DNA in the extracted sample. A staining method that facilitates the targeting of samples with a high content of unmodified DNA is provided.

Keywords


tetramethylsilane; ethidium bromide; formalin; Carcinus; Lithodidae

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