Bacterial community structure of mangrove sediments in relation to environmental variables accessed by 16S rRNA gene-denaturing gradient gel electrophoresis fingerprinting

Authors

  • Yanying Zhang South China Sea Institute of Oceanology, Chinese Academy of Sciences
  • Junde Dong South China Sea Institute of Oceanology, Chinese Academy of Sciences
  • Bin Yang South China Sea Institute of Oceanology, Chinese Academy of Sciences
  • Juan Ling South China Sea Institute of Oceanology, Chinese Academy of Sciences
  • Youshao Wang South China Sea Institute of Oceanology, Chinese Academy of Sciences
  • Si Zhang South China Sea Institute of Oceanology, Chinese Academy of Sciences

DOI:

https://doi.org/10.3989/scimar.2009.73n3487

Keywords:

bacterial community, mangrove sediments, denaturing gradient gel electrophoresis, multivariate statistics

Abstract


Bacterial community structure and the relationship between environmental variables and microbial communities in the surface sediments of tropical mangrove ecosystems were investigated in Sanya, Hainan Island, China. Profiles of bacterial communities were generated using culture-independent PCR-denaturing gradient gel electrophoresis (DGGE), and the results were interpreted with multivariate statistical analysis. Findings suggested that microbial communities varied with sample collection sites and seasons. The samples collected from the same sample sites at the same time had more similar microbial communities except samples SH3 and AB5, which also had unique sediment quality. Canonical correspondence analysis (CCA) revealed that the organic carbon concentration of the sediments accounted for a significant amount of the variability in the bacterial community composition. Phylogenetic analysis was used to identify the major groups of the predominant bacterial phylotypes. 16S rRNA gene-V3 fragments from 17 individual DGGE bands were sequenced and the corresponding bacteria were found in mangrove sediments for the first time based on BLAST results. Predominant bacterial phylotypes clustered with various taxonomic groups, including Proteobacteria, Bacteroidetes, Gemmatimonadetes, Actinobacteria and Firmicutes.

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Author Biographies

Yanying Zhang, South China Sea Institute of Oceanology, Chinese Academy of Sciences

National Experiment Station of Tropical Marine Biology, China Key Laboratory of Marine Bio-resources Sustainable Utilisation (LMB), China Graduate School of Chinese Academy of Science, Beijing, P. R. China

Junde Dong, South China Sea Institute of Oceanology, Chinese Academy of Sciences

National Experiment Station of Tropical Marine Biology, China Key Laboratory of Marine Bio-resources Sustainable Utilisation (LMB), China

Bin Yang, South China Sea Institute of Oceanology, Chinese Academy of Sciences

National Experiment Station of Tropical Marine Biology, China Key Laboratory of Marine Bio-resources Sustainable Utilisation (LMB), China

Juan Ling, South China Sea Institute of Oceanology, Chinese Academy of Sciences

National Experiment Station of Tropical Marine Biology, China. Key Laboratory of Marine Bio-resources Sustainable Utilisation (LMB), China Graduate School of Chinese Academy of Science, Beijing, P. R. China

Si Zhang, South China Sea Institute of Oceanology, Chinese Academy of Sciences

Key Laboratory of Marine Bio-resources Sustainable Utilisation (LMB), China

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Published

2009-09-30

How to Cite

1.
Zhang Y, Dong J, Yang B, Ling J, Wang Y, Zhang S. Bacterial community structure of mangrove sediments in relation to environmental variables accessed by 16S rRNA gene-denaturing gradient gel electrophoresis fingerprinting. Sci. mar. [Internet]. 2009Sep.30 [cited 2024Mar.28];73(3):487-98. Available from: https://scientiamarina.revistas.csic.es/index.php/scientiamarina/article/view/1079

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