Arnoglossus nigrofilamentosus n . sp . , a new species of flounder ( Teleostei : Bothidae ) from off the Mediterranean coast of Israel , probably a new case of Lessepsian migration

1 Im Ramstal 76, 97922 Lauda-Königshofen, Germany. (RF) (Corresponding author) E-mail: ronfricke@web.de. ORCID iD: http://orcid.org/0000-0003-1476-6990 2 National Natural History Collections and Department of Ecology, Evolution and Behavior, The Hebrew University of Jerusalem, 91904 Jerusalem, Israel. (DG) E-mail: dani.golani@mail.huji.ac.il. ORCID iD: http://orcid.org/0000-0003-4575-3324 3 Mt. Scopus Library, The Hebrew University of Jerusalem, 91905 Jerusalem, Israel. (BA-G) E-mail: brendag@savion.huji.ac.il. ORCID iD: http://orcid.org/0000-0002-3237-6980


INTRODUCTION
The lefteye flounders of the family Bothidae are a large group of marine fishes living in tropical and temperate waters of all oceans.They are arranged in the order Pleuronectiformes (Nelson et al. 2016), and currently contain a total of 169 valid species (Eschmeyer and Fong 2017).The Bothinae (now classified as the family Bothidae) were characterized by Norman (1934) as left-eye flounders with the pelvic fin on the blind side short-based, that on the ocular side elongate, extending forward to urohyal, supported by a cartilaginous plate placed in advance of the cleithra, its anterior ray well in advance of first ray of that of the blind side, and caudal vertebrae with well-developed apophyses.
The genus Arnoglossus Bleeker, 1862 was defined by Amaoka (1969) by having the pelvic fin on the ocular side beginning at the tip of the isthmus, the eyes separated by a bony ridge or a very narrow concave space in the anterior half, interorbital equally wide in both sexes, head large, more than 22% of SL, the scales on the ocular side cycloid or feebly ctenoid, and the mouth large, maxillary longer than eye diameter.The genus is distributed in the eastern Atlantic and the Indo-Pacific; it currently includes a total of 34 valid species (Eschmeyer et al. 2017); a list of these species and their distribution is provided in Table 1.
While examining the catch of R/V Bilu collected off Jaffa, Israel in the morning of 5 May 2017, two of the authors (RF and DG) discovered four specimens of an unusual species of Arnoglossus, that was subsequently found to be an undescribed species.This species is described herein, and its probable origin is discussed.

MATERIALS AND METHODS
The specimens of the new species were trawled during the night of 4-5 May 2017 by R/V Bilu off Jaffa, Israel, eastern Mediterranean Sea, at a depth of approximately 20-40 m judging from the composition of other species contained in the same haul.
Counts and measurements follow Hubbs and Lagler (1947), and descriptive methods follow Arai and Amaoka (1996) except for the caudal-fin ray formula, which follows Fricke (1983); the classification follows Eschmeyer et al. (2017), and the references are according to Fricke (2017).In the description, data of the holotype are presented first, followed by those of the paratypes, in parentheses.Diagnosis.A species of Arnoglossus with the second to sixth dorsal-fin rays elongate and filamentous, dorsal-fin rays 78-84, anal-fin rays 61-66, pectoral-fin rays on ocular side 12-13, on blind side 7-9, caudal-fin rays iii,11,iii , lateral-line scales 52-54, gill rakers 0 + 4-5, not serrated, interorbital a narrow bony ridge without scales in the middle, interorbital width 11% to 16% of upper orbit diameter, no enlarged teeth anteriorly in upper jaw, and prevomer small, not enlarged, weakly projecting into mouth cavity; body pale (may have been dark before the epidermis was abrased), head, peritoneum and vertical fins black.
Description.Counts and measurements of the holotype and paratypes listed in Table 2 are part of this description.
Colour of fresh specimen (Fig. 1).Head and body pale yellowish (may have been dark before the epidermis was abrased), head black, peritoneum dark.Dorsal-fin filaments, rays and membranes black; anal fin black, membranes distal three-fourths of caudal fin black.Pectoral fins yellowish, pelvic fin on ocular side blackish, on blind side pale.
Etymology.In the scientific name filamentosus means filamentous, and niger means black; the name refers to the black filamentous anterior dorsal-fin rays.
Distribution.So far, this new species is only known from off Jaffa, on the eastern Mediterranean coast of Israel (Fig. 3).However, as this species has not been found in the Mediterranean Sea before, and as it has close similarities with Indo-Pacific species, it is probable that it originally came from the Gulf of Suez (Red Sea), and is another case of Lessepsian migration.
Comparison.Arnoglossus nigrofilamentosus n. sp. is compared with other similar species bearing elongate, filamentous rays on the anterior dorsal fin in Table 3; the filament patterns are compared in Figure 4.It is distinguished from the other species of the genus by the presence of filamentous dorsal-fin rays.Among the Mediterranean species of Arnoglossus, this new species is very distinctive in having a low number of 0+4-5 gill rakers (versus 0+7-13 in the other species), the 2nd to 6th anterior dorsal-fin rays elongate and bearing black filaments (versus anterior dorsal-fin rays either not elongate and filamentous, or a single ray elongate and bearing a black filament in male A. thori, or else several anterior rays with long white filaments in A. imperialis), and the dorsal and anal fins black (versus pale, sometimes with spots or only the anterior rays black).For easy distinguishing of co-occurring species like A. grohmanni, A. laterna, A. rueppellii and A. thori, a key to the species of Arnoglossus of the Mediterranean and eastern Atlantic is provided below.
Compared with the Red Sea and western Indian Ocean species of the genus, the new species is also unique in having a low number of 78-84 dorsal-fin rays (versus 91-110), 61-66 anal-fin rays (versus 70-86) and 0+4-5 gill rakers (versus 0+6-13); it also differs from other species in having the anterior teeth of the upper jaws not enlarged (versus enlarged in A. dalgleishi, A. kotthausi and A. sayaensis), and the prevomer small, barely projecting into the mouth cavity (versus large, strongly projecting into the mouth cavity in A. macrolophus).It is distinguished from A. marisrubri by the presence of filamentous rays on the dorsal fin (versus no filamentous rays on the dorsal fin), dorsal-fin rays 78 to 84 (versus 95 to 97), anal-fin rays 61 to 66 (versus 74 to 77), and gill rakers 0+4-5 (versus 0+11-13).A key to distinguishing the species of Arnoglossus of the Red Sea and the western Indian Ocean is provided below.imperialis (male).E, A. imperialis (female).F, A. macrolophus (male).G, A. macrolophus (female).H, A. thori (male).I, A. thori (female).

DISCUSSION
The specimens described herein agree well with the generic characters of the genus Arnoglossus as defined by Norman (1934) and Amaoka (1969), including the pelvic fin on the ocular side beginning at the tip of the isthmus, the eyes separated by a bony ridge, the head large, more than 22% of SL (23.2%-24.7%), the scales on the ocular side cycloid, and the mouth large, maxillary longer than eye diameter.However, they do not agree with any of the known species of Arnoglossus from the Mediterranean/eastern Atlantic, from the Red Sea/ Indian Ocean, or from any other region.
This finding of a new species of Arnoglossus from off the Mediterranean coast of Israel is quite surprising, as there has been an extensive fishing effort in this habitat for many decades.However, the species was previously not detected and there is no material available in the regional ichthyological collections, so it is unlikely that it was just missed.The close similarity of this new species to other species occurring in the central Red Sea and the northwestern Indian Ocean (A. arabicus, A. macrolophus and A. marisrubri), and the obvious previous absence from the southeastern Mediterranean, makes it likely that this represents another case of Lessepsian migration, and that A. nigrofilamentosus n. sp.originates from the Gulf of Suez.Unfortunately, the Gulf of Suez ichthyofauna is incompletely known and under-represented in ichthyological collections, which makes it more likely that the new species was previously missed in that region.
The opening of the Suez Canal in 1869 connected the Red Sea with the Mediterranean, resulting in an almost unidirectional migration of Red Sea organisms into the Mediterranean ("Lessepsian migration") (Golani et al. 2002).A complete list of species to 2010 was provided by Golani (2010).Fricke et al. (2015) provided a list of additional Lessepsian migrant species, which resulted in a total of 97 migrant species confirmed in the Mediterranean.Galil et al. (2015) discussed the recent widening of the Suez Canal and the probability of increased Lessepsian migration.Russell et al. (2015) discovered that the species previously misidentified as Saurida undosquamis (non Richardson, 1848) or S. macrolepis (non Tanaka, 1917) from the northern Red Sea and the Mediter-

Table 1 .
of SL].Head length 4.0(4.1-4.3)inofSL].Snout slightly pointed, completely scaled.Anterior teeth in upper jaw not enlarged, prevomer not enlarged, weakly projecting into mouth cavity.Both nostrils situated anterior to the eyes.-Valid species in the genus Arnoglossus Bleeker, 1862 and their distribution, arranged according to their mean depth distribution.EA, eastern Atlantic; EI, eastern Indian Ocean; EP, eastern Pacific; MS, Mediterranean Sea; RS, Red Sea; WA, western Atlantic; WI, western Indian Ocean; WP, western Pacific.

Table 3 .
-Comparison of the species of Arnoglossus with several elongate, filamentous rays anteriorly in the dorsal fin, and the closely related species A. tapeinosoma (some values taken from Arai and Amaoka 1996, Hensley and Amaoka 2001, Munroe 2016).Values differing from A. nigrofilamentosus n. sp. are printed in bold face.